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Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

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Related in: MedlinePlus

Multiplexed dye delivery. (a) Calcein uptake was induced in the vertical bar area (green channel). (b) Dextran-tetramethylrhodamine uptake was induced in the horizontal bar area (yellow channel). (c) Delivery for both calcein and rhodamine was induced in the 4 corners of the square pattern in the cell culture. (d) Phase contrast image shows a confluent monolayer of viable HeLa cells that were imaged by immunofluorescence microscopy in panels (a-c). Bar = 100 µm.
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g007: Multiplexed dye delivery. (a) Calcein uptake was induced in the vertical bar area (green channel). (b) Dextran-tetramethylrhodamine uptake was induced in the horizontal bar area (yellow channel). (c) Delivery for both calcein and rhodamine was induced in the 4 corners of the square pattern in the cell culture. (d) Phase contrast image shows a confluent monolayer of viable HeLa cells that were imaged by immunofluorescence microscopy in panels (a-c). Bar = 100 µm.

Mentions: In Fig. 7Fig. 7


Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Multiplexed dye delivery. (a) Calcein uptake was induced in the vertical bar area (green channel). (b) Dextran-tetramethylrhodamine uptake was induced in the horizontal bar area (yellow channel). (c) Delivery for both calcein and rhodamine was induced in the 4 corners of the square pattern in the cell culture. (d) Phase contrast image shows a confluent monolayer of viable HeLa cells that were imaged by immunofluorescence microscopy in panels (a-c). Bar = 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369548&req=5

g007: Multiplexed dye delivery. (a) Calcein uptake was induced in the vertical bar area (green channel). (b) Dextran-tetramethylrhodamine uptake was induced in the horizontal bar area (yellow channel). (c) Delivery for both calcein and rhodamine was induced in the 4 corners of the square pattern in the cell culture. (d) Phase contrast image shows a confluent monolayer of viable HeLa cells that were imaged by immunofluorescence microscopy in panels (a-c). Bar = 100 µm.
Mentions: In Fig. 7Fig. 7

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

Show MeSH
Related in: MedlinePlus