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Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

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Related in: MedlinePlus

(a,c) Calcein delivery efficiency and cell viability under varying laser fluences (only irradiated with 1 pulse in each case). (b,d) Efficiency and viability under varying applied laser pulse numbers at 60 mJ/cm2 for HeLa (b) and at 36 mJ/cm2 for HEK 293T (d). The viability and efficiency values are represented as mean ± standard deviation. Number of experiments conducted are n=3 for HeLa and n=1 for HEK 293T.
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g006: (a,c) Calcein delivery efficiency and cell viability under varying laser fluences (only irradiated with 1 pulse in each case). (b,d) Efficiency and viability under varying applied laser pulse numbers at 60 mJ/cm2 for HeLa (b) and at 36 mJ/cm2 for HEK 293T (d). The viability and efficiency values are represented as mean ± standard deviation. Number of experiments conducted are n=3 for HeLa and n=1 for HEK 293T.

Mentions: Molecular delivery efficiency and cell viability assays in HeLa cells under (a) varying laser fluences (only irradiated with 1 pulse in each case) and (b) varying applied laser pulse numbers. Fluorescent images showing calcein (green) uptake within the laser irradiation pattern and propidium iodide (red) staining of dead cells. Bar = 50 µm.


Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

(a,c) Calcein delivery efficiency and cell viability under varying laser fluences (only irradiated with 1 pulse in each case). (b,d) Efficiency and viability under varying applied laser pulse numbers at 60 mJ/cm2 for HeLa (b) and at 36 mJ/cm2 for HEK 293T (d). The viability and efficiency values are represented as mean ± standard deviation. Number of experiments conducted are n=3 for HeLa and n=1 for HEK 293T.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369548&req=5

g006: (a,c) Calcein delivery efficiency and cell viability under varying laser fluences (only irradiated with 1 pulse in each case). (b,d) Efficiency and viability under varying applied laser pulse numbers at 60 mJ/cm2 for HeLa (b) and at 36 mJ/cm2 for HEK 293T (d). The viability and efficiency values are represented as mean ± standard deviation. Number of experiments conducted are n=3 for HeLa and n=1 for HEK 293T.
Mentions: Molecular delivery efficiency and cell viability assays in HeLa cells under (a) varying laser fluences (only irradiated with 1 pulse in each case) and (b) varying applied laser pulse numbers. Fluorescent images showing calcein (green) uptake within the laser irradiation pattern and propidium iodide (red) staining of dead cells. Bar = 50 µm.

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

Show MeSH
Related in: MedlinePlus