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Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

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Related in: MedlinePlus

Molecular delivery efficiency and cell viability assays in HeLa cells under (a) varying laser fluences (only irradiated with 1 pulse in each case) and (b) varying applied laser pulse numbers. Fluorescent images showing calcein (green) uptake within the laser irradiation pattern and propidium iodide (red) staining of dead cells. Bar = 50 µm.
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g005: Molecular delivery efficiency and cell viability assays in HeLa cells under (a) varying laser fluences (only irradiated with 1 pulse in each case) and (b) varying applied laser pulse numbers. Fluorescent images showing calcein (green) uptake within the laser irradiation pattern and propidium iodide (red) staining of dead cells. Bar = 50 µm.

Mentions: The efficiency of molecule delivery into cultured cells in general increases with irradiation laser fluence and the number of laser pulses applied. This can be rationalized by a larger pore formation on the cell membrane due to larger cavitation bubbles and the number of times the pores are induced. However, the disruption of the cell membrane needs to be kept at a minimum to avoid irreversible damage that leads to cell death [16]. We systematically varied the laser fluence and the applied pulse number to evaluate uptake efficiency and the corresponding cell viability. Figure 5Fig. 5


Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Molecular delivery efficiency and cell viability assays in HeLa cells under (a) varying laser fluences (only irradiated with 1 pulse in each case) and (b) varying applied laser pulse numbers. Fluorescent images showing calcein (green) uptake within the laser irradiation pattern and propidium iodide (red) staining of dead cells. Bar = 50 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369548&req=5

g005: Molecular delivery efficiency and cell viability assays in HeLa cells under (a) varying laser fluences (only irradiated with 1 pulse in each case) and (b) varying applied laser pulse numbers. Fluorescent images showing calcein (green) uptake within the laser irradiation pattern and propidium iodide (red) staining of dead cells. Bar = 50 µm.
Mentions: The efficiency of molecule delivery into cultured cells in general increases with irradiation laser fluence and the number of laser pulses applied. This can be rationalized by a larger pore formation on the cell membrane due to larger cavitation bubbles and the number of times the pores are induced. However, the disruption of the cell membrane needs to be kept at a minimum to avoid irreversible damage that leads to cell death [16]. We systematically varied the laser fluence and the applied pulse number to evaluate uptake efficiency and the corresponding cell viability. Figure 5Fig. 5

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

Show MeSH
Related in: MedlinePlus