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Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

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Pulsed laser annealing of gold films. (a) Schematic of the formation of gold particles. (b) SEM images of the 10 nm gold film annealed at 49.5 mJ/cm2. (c) Measured particle size distribution. Bar=5 µm.
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g003: Pulsed laser annealing of gold films. (a) Schematic of the formation of gold particles. (b) SEM images of the 10 nm gold film annealed at 49.5 mJ/cm2. (c) Measured particle size distribution. Bar=5 µm.

Mentions: Pulsed laser annealing of metal thin films has been investigated [6,7]. It allows for large area annealing in a room temperature environment while minimizing substrate heating. This way the top layer can be effectively annealed even on a low-melting-point substrate, such as plastic [8]. During the laser pulse irradiation, the metal layer rapidly heats up. For metals which have poor wetting properties on the substrate (e.g. gold on SiO2), instability and capillary flow drive the molten thin film to break up into droplets and form metal particles as the droplets re-solidify. For 10 nm thick gold films, the measured particle diameter distribution showed two peaks (Fig. 3(c)Fig. 3


Image patterned molecular delivery into live cells using gold particle coated substrates.

Wu TH, Kalim S, Callahan C, Teitell MA, Chiou PY - Opt Express (2010)

Pulsed laser annealing of gold films. (a) Schematic of the formation of gold particles. (b) SEM images of the 10 nm gold film annealed at 49.5 mJ/cm2. (c) Measured particle size distribution. Bar=5 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369548&req=5

g003: Pulsed laser annealing of gold films. (a) Schematic of the formation of gold particles. (b) SEM images of the 10 nm gold film annealed at 49.5 mJ/cm2. (c) Measured particle size distribution. Bar=5 µm.
Mentions: Pulsed laser annealing of metal thin films has been investigated [6,7]. It allows for large area annealing in a room temperature environment while minimizing substrate heating. This way the top layer can be effectively annealed even on a low-melting-point substrate, such as plastic [8]. During the laser pulse irradiation, the metal layer rapidly heats up. For metals which have poor wetting properties on the substrate (e.g. gold on SiO2), instability and capillary flow drive the molten thin film to break up into droplets and form metal particles as the droplets re-solidify. For 10 nm thick gold films, the measured particle diameter distribution showed two peaks (Fig. 3(c)Fig. 3

Bottom Line: An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer.Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein.Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

ABSTRACT
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.

Show MeSH
Related in: MedlinePlus