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Antimicrobial Constituents of Artemisia afra Jacq. ex Willd. against Periodontal Pathogens.

More G, Lall N, Hussein A, Tshikalange TE - Evid Based Complement Alternat Med (2012)

Bottom Line: Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC(50) value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL).Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC(50) values of 35.44 and 30.96 μg/mL.The obtained results in this study confirm the use of A. afra in the treatment of microbial infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Science, University of Pretoria, Pretoria 0002, South Africa.

ABSTRACT
The phytochemical investigation of an ethanol extract of Artemisia afra led to the isolation of six known compounds, acacetin (1), 12α,4α-dihydroxybishopsolicepolide (2), scopoletin (3), α-amyrin (4), phytol (5), and a pentacyclic triterpenoid betulinic acid (6). The compounds were evaluated for antimicrobial activity against Gram positive (Actinomyces naeslundii, Actinomyces israelii, and Streptococcus mutans), Gram negative bacteria (Prevotella intermedia, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans previously known as Actinobacillus actinomycetemcomitans), and Candida albicans. The crude extract of A. afra inhibited the growth of all tested microbial species at concentration range of 1.6 mg/mL to 25 mg/mL. The compounds 1-6 also showed activity range at 1.0 mg/mL to 0.25 mg/mL. Three best compounds (scopoletin, betulinic acid, and acacetin) which showed good antimicrobial activity were selected for further studies. Cytotoxicity of extract and compounds was determined using the XTT cell proliferation kit. The antioxidant activity of the extract and compounds was done using the DPPH scavenging method. The extract showed good antioxidant activity with an IC(50) value of 22.2 μg/mL. Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC(50) value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL). Acacetin and betulinic acid exhibited a decreased scavenging activity with the IC(50) of 2.39 and 2.42 μg/mL, respectively. The extract and compounds showed moderate toxicity on McCoy fibroblast cell line and scopoletin was relatively nontoxic with an IC(50) value of 132.5 μg/mL. Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC(50) values of 35.44 and 30.96 μg/mL. The obtained results in this study confirm the use of A. afra in the treatment of microbial infections.

No MeSH data available.


Related in: MedlinePlus

The DPPH inhibitory activities of the isolated compounds and vitamin C.
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Related In: Results  -  Collection


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fig2: The DPPH inhibitory activities of the isolated compounds and vitamin C.

Mentions: The antioxidant activity of three selected compounds (scopoletin, acacetin and betulinic acid) revealed that they are effective antioxidant agents (Figure 2). Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC50 value of 1.24 μg/mL which was in close range to that of vitamin C (1.22 μg/mL). Acacetin and Betulinic acid exhibited a slightly low DPPH scavenging activity with the IC50 of 2.39 and 2.42 μg/mL, respectively.


Antimicrobial Constituents of Artemisia afra Jacq. ex Willd. against Periodontal Pathogens.

More G, Lall N, Hussein A, Tshikalange TE - Evid Based Complement Alternat Med (2012)

The DPPH inhibitory activities of the isolated compounds and vitamin C.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369547&req=5

fig2: The DPPH inhibitory activities of the isolated compounds and vitamin C.
Mentions: The antioxidant activity of three selected compounds (scopoletin, acacetin and betulinic acid) revealed that they are effective antioxidant agents (Figure 2). Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC50 value of 1.24 μg/mL which was in close range to that of vitamin C (1.22 μg/mL). Acacetin and Betulinic acid exhibited a slightly low DPPH scavenging activity with the IC50 of 2.39 and 2.42 μg/mL, respectively.

Bottom Line: Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC(50) value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL).Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC(50) values of 35.44 and 30.96 μg/mL.The obtained results in this study confirm the use of A. afra in the treatment of microbial infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Science, University of Pretoria, Pretoria 0002, South Africa.

ABSTRACT
The phytochemical investigation of an ethanol extract of Artemisia afra led to the isolation of six known compounds, acacetin (1), 12α,4α-dihydroxybishopsolicepolide (2), scopoletin (3), α-amyrin (4), phytol (5), and a pentacyclic triterpenoid betulinic acid (6). The compounds were evaluated for antimicrobial activity against Gram positive (Actinomyces naeslundii, Actinomyces israelii, and Streptococcus mutans), Gram negative bacteria (Prevotella intermedia, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans previously known as Actinobacillus actinomycetemcomitans), and Candida albicans. The crude extract of A. afra inhibited the growth of all tested microbial species at concentration range of 1.6 mg/mL to 25 mg/mL. The compounds 1-6 also showed activity range at 1.0 mg/mL to 0.25 mg/mL. Three best compounds (scopoletin, betulinic acid, and acacetin) which showed good antimicrobial activity were selected for further studies. Cytotoxicity of extract and compounds was determined using the XTT cell proliferation kit. The antioxidant activity of the extract and compounds was done using the DPPH scavenging method. The extract showed good antioxidant activity with an IC(50) value of 22.2 μg/mL. Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC(50) value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL). Acacetin and betulinic acid exhibited a decreased scavenging activity with the IC(50) of 2.39 and 2.42 μg/mL, respectively. The extract and compounds showed moderate toxicity on McCoy fibroblast cell line and scopoletin was relatively nontoxic with an IC(50) value of 132.5 μg/mL. Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC(50) values of 35.44 and 30.96 μg/mL. The obtained results in this study confirm the use of A. afra in the treatment of microbial infections.

No MeSH data available.


Related in: MedlinePlus