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Molecular Mechanisms of Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease in Chronic Hepatitis B and Liver Cirrhosis.

Guo Z, Yu S, Guan Y, Li YY, Lu YY, Zhang H, Su SB - Evid Based Complement Alternat Med (2012)

Bottom Line: The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway.Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease.The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Complex System of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.

ABSTRACT
Traditional Chinese medicine (TCM) treatment is based on the traditional diagnose method to distinguish the TCM syndrome, not the disease. So there is a phenomenon in the relationship between TCM syndrome and disease, called Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease. In this study, we demonstrated the molecular mechanisms of this phenomenon using the microarray samples of liver-gallbladder dampness-heat syndrome (LGDHS) and liver depression and spleen deficiency syndrome (LDSDS) in the chronic hepatitis B (CHB) and liver cirrhosis (LC). The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway. Therein genes GPER, PTHR1, GPR173, and SSTR1 were coexpressed in LDSDS, but not in LGDHS. Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease. The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

No MeSH data available.


Related in: MedlinePlus

Average expression and correlation of DRD5 GABRA SSTR1 and NPFF mRNAs in diseases and TCM syndromes. The gene expression levels of both LGDHS and LDSDS were lower in CHB and that of LDSDS was more than LGDHS in LC (a). (Gene expression levels were the ratio of each mRNA and ACTB mRNA). The correlation coefficient of LDSDS in CHB and LC was more than LGDHS in CHB and LC (b).
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fig6: Average expression and correlation of DRD5 GABRA SSTR1 and NPFF mRNAs in diseases and TCM syndromes. The gene expression levels of both LGDHS and LDSDS were lower in CHB and that of LDSDS was more than LGDHS in LC (a). (Gene expression levels were the ratio of each mRNA and ACTB mRNA). The correlation coefficient of LDSDS in CHB and LC was more than LGDHS in CHB and LC (b).

Mentions: To test and verify the difference of average expression level and correlation of genes in GCRP pathway, DRD5 GABRA SSTR1 and NPFF mRNAs were expressed by real-time RT-PCR. The average expression levels of these genes in both LGDHS and LDSDS were lower in CHB, and that of LDSDS was more than LGDHS in LC (Figure 6(a)). The correlation coefficient of LDSDS (>0.5) in CHB and LC was more than LGDHS (<0.5) in CHB and LC (Figure 6(b)). These results further confirmed that the gene expression level was lower in CHB and higher or lower in LC. The genes in LDSDS had higher correlation than LGDHS whether disease was CHB or LC.


Molecular Mechanisms of Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease in Chronic Hepatitis B and Liver Cirrhosis.

Guo Z, Yu S, Guan Y, Li YY, Lu YY, Zhang H, Su SB - Evid Based Complement Alternat Med (2012)

Average expression and correlation of DRD5 GABRA SSTR1 and NPFF mRNAs in diseases and TCM syndromes. The gene expression levels of both LGDHS and LDSDS were lower in CHB and that of LDSDS was more than LGDHS in LC (a). (Gene expression levels were the ratio of each mRNA and ACTB mRNA). The correlation coefficient of LDSDS in CHB and LC was more than LGDHS in CHB and LC (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369542&req=5

fig6: Average expression and correlation of DRD5 GABRA SSTR1 and NPFF mRNAs in diseases and TCM syndromes. The gene expression levels of both LGDHS and LDSDS were lower in CHB and that of LDSDS was more than LGDHS in LC (a). (Gene expression levels were the ratio of each mRNA and ACTB mRNA). The correlation coefficient of LDSDS in CHB and LC was more than LGDHS in CHB and LC (b).
Mentions: To test and verify the difference of average expression level and correlation of genes in GCRP pathway, DRD5 GABRA SSTR1 and NPFF mRNAs were expressed by real-time RT-PCR. The average expression levels of these genes in both LGDHS and LDSDS were lower in CHB, and that of LDSDS was more than LGDHS in LC (Figure 6(a)). The correlation coefficient of LDSDS (>0.5) in CHB and LC was more than LGDHS (<0.5) in CHB and LC (Figure 6(b)). These results further confirmed that the gene expression level was lower in CHB and higher or lower in LC. The genes in LDSDS had higher correlation than LGDHS whether disease was CHB or LC.

Bottom Line: The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway.Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease.The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Complex System of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.

ABSTRACT
Traditional Chinese medicine (TCM) treatment is based on the traditional diagnose method to distinguish the TCM syndrome, not the disease. So there is a phenomenon in the relationship between TCM syndrome and disease, called Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease. In this study, we demonstrated the molecular mechanisms of this phenomenon using the microarray samples of liver-gallbladder dampness-heat syndrome (LGDHS) and liver depression and spleen deficiency syndrome (LDSDS) in the chronic hepatitis B (CHB) and liver cirrhosis (LC). The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway. Therein genes GPER, PTHR1, GPR173, and SSTR1 were coexpressed in LDSDS, but not in LGDHS. Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease. The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

No MeSH data available.


Related in: MedlinePlus