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Molecular Mechanisms of Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease in Chronic Hepatitis B and Liver Cirrhosis.

Guo Z, Yu S, Guan Y, Li YY, Lu YY, Zhang H, Su SB - Evid Based Complement Alternat Med (2012)

Bottom Line: The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway.Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease.The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Complex System of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.

ABSTRACT
Traditional Chinese medicine (TCM) treatment is based on the traditional diagnose method to distinguish the TCM syndrome, not the disease. So there is a phenomenon in the relationship between TCM syndrome and disease, called Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease. In this study, we demonstrated the molecular mechanisms of this phenomenon using the microarray samples of liver-gallbladder dampness-heat syndrome (LGDHS) and liver depression and spleen deficiency syndrome (LDSDS) in the chronic hepatitis B (CHB) and liver cirrhosis (LC). The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway. Therein genes GPER, PTHR1, GPR173, and SSTR1 were coexpressed in LDSDS, but not in LGDHS. Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease. The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

No MeSH data available.


Related in: MedlinePlus

Heatmap of 98 differentially expressed genes between LGDHS and LDSDS. The 98 differentially expressed genes between LGDHS and LDSDS were obviously divided out by Heatmap analysis. Row: genes; column: patient number; deep colour: upexpressed genes; light colour: down-expressed genes; A1–3 and D1–3: LDSDS; B 4, 5 and E4–6: LGDHS.
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fig1: Heatmap of 98 differentially expressed genes between LGDHS and LDSDS. The 98 differentially expressed genes between LGDHS and LDSDS were obviously divided out by Heatmap analysis. Row: genes; column: patient number; deep colour: upexpressed genes; light colour: down-expressed genes; A1–3 and D1–3: LDSDS; B 4, 5 and E4–6: LGDHS.

Mentions: At first, to find whether there were some significant genes that could characterize the difference between two disease and two TCM syndromes, t-test was used to select difference expression gene in both disease and TCM syndrome levels. The threshold was P value less than 0.01. Remarkably, 6579 in all 14352 genes were differentially expressed between CHB and LC, suggested that the difference in mRNA expression level was very clear, according to CHB and LC that were completely different diseases. In contrast, only 98 genes were differentially expressed between LGDHS and LDSDS. The heatmap of the 98 genes between LGDHS and LDSDS was showed in Figure 1. Moreover, though these genes were obviously differentiated into two syndromes, the 98 genes were in disorder, no significantly related function was found by GO enrichment analysis. It also was tried to change the threshold as P value less than 0.05 and got 830 genes, but still any significantly related GO function was not found.


Molecular Mechanisms of Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease in Chronic Hepatitis B and Liver Cirrhosis.

Guo Z, Yu S, Guan Y, Li YY, Lu YY, Zhang H, Su SB - Evid Based Complement Alternat Med (2012)

Heatmap of 98 differentially expressed genes between LGDHS and LDSDS. The 98 differentially expressed genes between LGDHS and LDSDS were obviously divided out by Heatmap analysis. Row: genes; column: patient number; deep colour: upexpressed genes; light colour: down-expressed genes; A1–3 and D1–3: LDSDS; B 4, 5 and E4–6: LGDHS.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369542&req=5

fig1: Heatmap of 98 differentially expressed genes between LGDHS and LDSDS. The 98 differentially expressed genes between LGDHS and LDSDS were obviously divided out by Heatmap analysis. Row: genes; column: patient number; deep colour: upexpressed genes; light colour: down-expressed genes; A1–3 and D1–3: LDSDS; B 4, 5 and E4–6: LGDHS.
Mentions: At first, to find whether there were some significant genes that could characterize the difference between two disease and two TCM syndromes, t-test was used to select difference expression gene in both disease and TCM syndrome levels. The threshold was P value less than 0.01. Remarkably, 6579 in all 14352 genes were differentially expressed between CHB and LC, suggested that the difference in mRNA expression level was very clear, according to CHB and LC that were completely different diseases. In contrast, only 98 genes were differentially expressed between LGDHS and LDSDS. The heatmap of the 98 genes between LGDHS and LDSDS was showed in Figure 1. Moreover, though these genes were obviously differentiated into two syndromes, the 98 genes were in disorder, no significantly related function was found by GO enrichment analysis. It also was tried to change the threshold as P value less than 0.05 and got 830 genes, but still any significantly related GO function was not found.

Bottom Line: The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway.Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease.The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Complex System of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.

ABSTRACT
Traditional Chinese medicine (TCM) treatment is based on the traditional diagnose method to distinguish the TCM syndrome, not the disease. So there is a phenomenon in the relationship between TCM syndrome and disease, called Same TCM Syndrome for Different Diseases and Different TCM Syndrome for Same Disease. In this study, we demonstrated the molecular mechanisms of this phenomenon using the microarray samples of liver-gallbladder dampness-heat syndrome (LGDHS) and liver depression and spleen deficiency syndrome (LDSDS) in the chronic hepatitis B (CHB) and liver cirrhosis (LC). The results showed that the difference between CHB and LC was gene expression level and the difference between LGDHS and LDSDS was gene coexpression in the G-protein-coupled receptor protein-signaling pathway. Therein genes GPER, PTHR1, GPR173, and SSTR1 were coexpressed in LDSDS, but not in LGDHS. Either CHB or LC was divided into the alternative LGDHS and LDSDS by the gene correlation, which reveals the molecular feature of Different TCM Syndrome for Same Disease. The alternatives LGDHS and LDSDS were divided into either CHB or LC by the gene expression level, which reveals the molecular feature of Same TCM Syndrome for Different Diseases.

No MeSH data available.


Related in: MedlinePlus