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A new piezoelectric actuator induces bone formation in vivo: a preliminary study.

Reis J, Frias C, Canto e Castro C, Botelho ML, Marques AT, Simões JA, Capela e Silva F, Potes J - J. Biomed. Biotechnol. (2012)

Bottom Line: After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls.Bone deposition rate was also significantly higher in the mechanically stimulated areas.The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina Veterinária, Universidade de Évora, 7002-554 Évora, Portugal.

ABSTRACT
This in vivo study presents the preliminary results of the use of a novel piezoelectric actuator for orthopedic application. The innovative use of the converse piezoelectric effect to mechanically stimulate bone was achieved with polyvinylidene fluoride actuators implanted in osteotomy cuts in sheep femur and tibia. The biological response around the osteotomies was assessed through histology and histomorphometry in nondecalcified sections and histochemistry and immunohistochemistry in decalcified sections, namely, through Masson's trichrome, and labeling of osteopontin, proliferating cell nuclear antigen, and tartrate-resistant acid phosphatase. After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls. Bone deposition rate was also significantly higher in the mechanically stimulated areas. In these areas, osteopontin increased expression was observed. The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

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Microphotograph of decalcified sections, osteopontin (white arrows) and PCNA expression. Picture shows Z3 areas of femoral: (a) actuator and (b) static control, suggesting more extensive osteopontin labeling around actuator. Double Fast-Red and DAB immunohistochemistry staining for osteopontin and PCNA, respectively. Scale bar represents 100 μm.
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fig7: Microphotograph of decalcified sections, osteopontin (white arrows) and PCNA expression. Picture shows Z3 areas of femoral: (a) actuator and (b) static control, suggesting more extensive osteopontin labeling around actuator. Double Fast-Red and DAB immunohistochemistry staining for osteopontin and PCNA, respectively. Scale bar represents 100 μm.

Mentions: Immunohistochemistry failed to detect differences in TRAP expression between the two groups, but there is a marked elevation in OPN detection around actuators, in Z3 and Z4 (Figure 7). OPN is also strongly labeled in the fibroblast layers closer to the PVDF films, both in actuators and static controls, and this is the area where positive PCNA labeling was found more often. PCNA labeling was also positive in some of the preosteoblastic cells in layers apposed to OPN-positive columnar osteoblasts.


A new piezoelectric actuator induces bone formation in vivo: a preliminary study.

Reis J, Frias C, Canto e Castro C, Botelho ML, Marques AT, Simões JA, Capela e Silva F, Potes J - J. Biomed. Biotechnol. (2012)

Microphotograph of decalcified sections, osteopontin (white arrows) and PCNA expression. Picture shows Z3 areas of femoral: (a) actuator and (b) static control, suggesting more extensive osteopontin labeling around actuator. Double Fast-Red and DAB immunohistochemistry staining for osteopontin and PCNA, respectively. Scale bar represents 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369535&req=5

fig7: Microphotograph of decalcified sections, osteopontin (white arrows) and PCNA expression. Picture shows Z3 areas of femoral: (a) actuator and (b) static control, suggesting more extensive osteopontin labeling around actuator. Double Fast-Red and DAB immunohistochemistry staining for osteopontin and PCNA, respectively. Scale bar represents 100 μm.
Mentions: Immunohistochemistry failed to detect differences in TRAP expression between the two groups, but there is a marked elevation in OPN detection around actuators, in Z3 and Z4 (Figure 7). OPN is also strongly labeled in the fibroblast layers closer to the PVDF films, both in actuators and static controls, and this is the area where positive PCNA labeling was found more often. PCNA labeling was also positive in some of the preosteoblastic cells in layers apposed to OPN-positive columnar osteoblasts.

Bottom Line: After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls.Bone deposition rate was also significantly higher in the mechanically stimulated areas.The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina Veterinária, Universidade de Évora, 7002-554 Évora, Portugal.

ABSTRACT
This in vivo study presents the preliminary results of the use of a novel piezoelectric actuator for orthopedic application. The innovative use of the converse piezoelectric effect to mechanically stimulate bone was achieved with polyvinylidene fluoride actuators implanted in osteotomy cuts in sheep femur and tibia. The biological response around the osteotomies was assessed through histology and histomorphometry in nondecalcified sections and histochemistry and immunohistochemistry in decalcified sections, namely, through Masson's trichrome, and labeling of osteopontin, proliferating cell nuclear antigen, and tartrate-resistant acid phosphatase. After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls. Bone deposition rate was also significantly higher in the mechanically stimulated areas. In these areas, osteopontin increased expression was observed. The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

Show MeSH
Related in: MedlinePlus