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A new piezoelectric actuator induces bone formation in vivo: a preliminary study.

Reis J, Frias C, Canto e Castro C, Botelho ML, Marques AT, Simões JA, Capela e Silva F, Potes J - J. Biomed. Biotechnol. (2012)

Bottom Line: After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls.Bone deposition rate was also significantly higher in the mechanically stimulated areas.The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina Veterinária, Universidade de Évora, 7002-554 Évora, Portugal.

ABSTRACT
This in vivo study presents the preliminary results of the use of a novel piezoelectric actuator for orthopedic application. The innovative use of the converse piezoelectric effect to mechanically stimulate bone was achieved with polyvinylidene fluoride actuators implanted in osteotomy cuts in sheep femur and tibia. The biological response around the osteotomies was assessed through histology and histomorphometry in nondecalcified sections and histochemistry and immunohistochemistry in decalcified sections, namely, through Masson's trichrome, and labeling of osteopontin, proliferating cell nuclear antigen, and tartrate-resistant acid phosphatase. After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls. Bone deposition rate was also significantly higher in the mechanically stimulated areas. In these areas, osteopontin increased expression was observed. The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

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Related in: MedlinePlus

Location of the actuators within the bone. The figure shows example of bone section prior to inclusion: (a) section of tibia—scale bar 1 cm; (b) shows how the areas for histomorphometry were distributed; Z1 corresponds to the film clamped region—scale bar 2 cm.
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fig1: Location of the actuators within the bone. The figure shows example of bone section prior to inclusion: (a) section of tibia—scale bar 1 cm; (b) shows how the areas for histomorphometry were distributed; Z1 corresponds to the film clamped region—scale bar 2 cm.

Mentions: A stab wound was made in the fascia lata, and the extremities of the piezoelectric films passed through it, and then, the active area of each piezoelectric film was placed inside the slot osteotomy cavity. The actuator position inside the tibial bone can be clearly viewed in Figure 1(a), obtained post-mortem. The portion of the device containing the microprocessor and the power supply was left in the nearby subcutaneous space.


A new piezoelectric actuator induces bone formation in vivo: a preliminary study.

Reis J, Frias C, Canto e Castro C, Botelho ML, Marques AT, Simões JA, Capela e Silva F, Potes J - J. Biomed. Biotechnol. (2012)

Location of the actuators within the bone. The figure shows example of bone section prior to inclusion: (a) section of tibia—scale bar 1 cm; (b) shows how the areas for histomorphometry were distributed; Z1 corresponds to the film clamped region—scale bar 2 cm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369535&req=5

fig1: Location of the actuators within the bone. The figure shows example of bone section prior to inclusion: (a) section of tibia—scale bar 1 cm; (b) shows how the areas for histomorphometry were distributed; Z1 corresponds to the film clamped region—scale bar 2 cm.
Mentions: A stab wound was made in the fascia lata, and the extremities of the piezoelectric films passed through it, and then, the active area of each piezoelectric film was placed inside the slot osteotomy cavity. The actuator position inside the tibial bone can be clearly viewed in Figure 1(a), obtained post-mortem. The portion of the device containing the microprocessor and the power supply was left in the nearby subcutaneous space.

Bottom Line: After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls.Bone deposition rate was also significantly higher in the mechanically stimulated areas.The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina Veterinária, Universidade de Évora, 7002-554 Évora, Portugal.

ABSTRACT
This in vivo study presents the preliminary results of the use of a novel piezoelectric actuator for orthopedic application. The innovative use of the converse piezoelectric effect to mechanically stimulate bone was achieved with polyvinylidene fluoride actuators implanted in osteotomy cuts in sheep femur and tibia. The biological response around the osteotomies was assessed through histology and histomorphometry in nondecalcified sections and histochemistry and immunohistochemistry in decalcified sections, namely, through Masson's trichrome, and labeling of osteopontin, proliferating cell nuclear antigen, and tartrate-resistant acid phosphatase. After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls. Bone deposition rate was also significantly higher in the mechanically stimulated areas. In these areas, osteopontin increased expression was observed. The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

Show MeSH
Related in: MedlinePlus