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Possible migration and histopathological analysis of injections of polymethylmethacrylate in wistar rats.

Neves Rd, Herdt MA, Wohlgemuth FB, Ely JB, de Vasconcellos ZA, Bastos JC, d'Acampora AJ - ISRN Dermatol (2012)

Bottom Line: Two animals had nonspecific spleen alterations.Conclusion.The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil.

ABSTRACT
Objective. To evaluate the possible migration of polymethylmethacrylate after injections in various corporal compartments of Wistar rats. Methods. The experimental work consisted in the injection of PMMA in corporal compartments for later histopathological analysis of the locations of implants and of distant filtering organs. The dose applied in each implant was of 0.2 mL. The animals were divided into groups according to the location of the implant realized: group GB had intradermic injections in the glabella. Group SD had subdermal injections in dorsal subcutaneous tissue cells. Group IP had intraperitoneal injections in the abdomen. Group PD had intramuscular injections in the right rear leg. The rats were sacrificed 30 days after realization of the implants and tissue samples from the lung, liver, spleen, and kidney, and locations of implantation were removed for histopathological analysis. Results. Characteristic microspheres that were compatible with the presence of PMMA in any of the histological slides analyzed were not observed. One animal had an amorphous exogenous substance, with a histiocytic reaction. Twelve of the 16 lungs analyzed had locations of intraalveolar hemorrhaging. Two animals had nonspecific spleen alterations. Conclusion. The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

No MeSH data available.


Related in: MedlinePlus

Microscopic images of muscle tissue without significant alterations. (a) Diaphragm (magnified 4x). (b) Rear right foot (magnified 10x).
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fig8: Microscopic images of muscle tissue without significant alterations. (a) Diaphragm (magnified 4x). (b) Rear right foot (magnified 10x).

Mentions: No alteration was found in the histological sections of the diaphragms (Figure 8(a)), liver, kidney, and spleen analyzed. In two animals, congestion was observed in the histological analysis of the spleen, even if the material compatible with microspheres was not found in these organs. In all the sections containing the lung tissue of the animals in this group, locations of intra-alveolar hemorrhaging were observed. In one of these animals, these alterations were extensive and significant (Table 2).


Possible migration and histopathological analysis of injections of polymethylmethacrylate in wistar rats.

Neves Rd, Herdt MA, Wohlgemuth FB, Ely JB, de Vasconcellos ZA, Bastos JC, d'Acampora AJ - ISRN Dermatol (2012)

Microscopic images of muscle tissue without significant alterations. (a) Diaphragm (magnified 4x). (b) Rear right foot (magnified 10x).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369493&req=5

fig8: Microscopic images of muscle tissue without significant alterations. (a) Diaphragm (magnified 4x). (b) Rear right foot (magnified 10x).
Mentions: No alteration was found in the histological sections of the diaphragms (Figure 8(a)), liver, kidney, and spleen analyzed. In two animals, congestion was observed in the histological analysis of the spleen, even if the material compatible with microspheres was not found in these organs. In all the sections containing the lung tissue of the animals in this group, locations of intra-alveolar hemorrhaging were observed. In one of these animals, these alterations were extensive and significant (Table 2).

Bottom Line: Two animals had nonspecific spleen alterations.Conclusion.The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil.

ABSTRACT
Objective. To evaluate the possible migration of polymethylmethacrylate after injections in various corporal compartments of Wistar rats. Methods. The experimental work consisted in the injection of PMMA in corporal compartments for later histopathological analysis of the locations of implants and of distant filtering organs. The dose applied in each implant was of 0.2 mL. The animals were divided into groups according to the location of the implant realized: group GB had intradermic injections in the glabella. Group SD had subdermal injections in dorsal subcutaneous tissue cells. Group IP had intraperitoneal injections in the abdomen. Group PD had intramuscular injections in the right rear leg. The rats were sacrificed 30 days after realization of the implants and tissue samples from the lung, liver, spleen, and kidney, and locations of implantation were removed for histopathological analysis. Results. Characteristic microspheres that were compatible with the presence of PMMA in any of the histological slides analyzed were not observed. One animal had an amorphous exogenous substance, with a histiocytic reaction. Twelve of the 16 lungs analyzed had locations of intraalveolar hemorrhaging. Two animals had nonspecific spleen alterations. Conclusion. The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

No MeSH data available.


Related in: MedlinePlus