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Possible migration and histopathological analysis of injections of polymethylmethacrylate in wistar rats.

Neves Rd, Herdt MA, Wohlgemuth FB, Ely JB, de Vasconcellos ZA, Bastos JC, d'Acampora AJ - ISRN Dermatol (2012)

Bottom Line: Two animals had nonspecific spleen alterations.Conclusion.The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil.

ABSTRACT
Objective. To evaluate the possible migration of polymethylmethacrylate after injections in various corporal compartments of Wistar rats. Methods. The experimental work consisted in the injection of PMMA in corporal compartments for later histopathological analysis of the locations of implants and of distant filtering organs. The dose applied in each implant was of 0.2 mL. The animals were divided into groups according to the location of the implant realized: group GB had intradermic injections in the glabella. Group SD had subdermal injections in dorsal subcutaneous tissue cells. Group IP had intraperitoneal injections in the abdomen. Group PD had intramuscular injections in the right rear leg. The rats were sacrificed 30 days after realization of the implants and tissue samples from the lung, liver, spleen, and kidney, and locations of implantation were removed for histopathological analysis. Results. Characteristic microspheres that were compatible with the presence of PMMA in any of the histological slides analyzed were not observed. One animal had an amorphous exogenous substance, with a histiocytic reaction. Twelve of the 16 lungs analyzed had locations of intraalveolar hemorrhaging. Two animals had nonspecific spleen alterations. Conclusion. The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

No MeSH data available.


Related in: MedlinePlus

Microscopic images. (a) Kidney tissue analyzed (magnified 10x). (b) Liver tissue (magnified 10x).
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fig5: Microscopic images. (a) Kidney tissue analyzed (magnified 10x). (b) Liver tissue (magnified 10x).

Mentions: For the analysis of local migration, specific organs were removed depending on the location of the injection. In group GB, the entire eyeball was removed with part of the attached optical muscles and nerve. In the animals of the SD group, the skin with the subcutaneous cell tissue from the implanted area was removed. In the IP and MPD groups, respectively, the diaphragm and musculature of the rear right leg were removed (Figure 5). These parts were then set in formaldehyde and placed in recipients identified by numbers corresponding to each rat and respective group. The histopathological analysis was conducted at the Instituto de Diagnóstico Anátomo Patológico (Institute of Pathological Anatomy Diagnostics) (IDAP), in the city of Florianópolis, by a single pathologist.


Possible migration and histopathological analysis of injections of polymethylmethacrylate in wistar rats.

Neves Rd, Herdt MA, Wohlgemuth FB, Ely JB, de Vasconcellos ZA, Bastos JC, d'Acampora AJ - ISRN Dermatol (2012)

Microscopic images. (a) Kidney tissue analyzed (magnified 10x). (b) Liver tissue (magnified 10x).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369493&req=5

fig5: Microscopic images. (a) Kidney tissue analyzed (magnified 10x). (b) Liver tissue (magnified 10x).
Mentions: For the analysis of local migration, specific organs were removed depending on the location of the injection. In group GB, the entire eyeball was removed with part of the attached optical muscles and nerve. In the animals of the SD group, the skin with the subcutaneous cell tissue from the implanted area was removed. In the IP and MPD groups, respectively, the diaphragm and musculature of the rear right leg were removed (Figure 5). These parts were then set in formaldehyde and placed in recipients identified by numbers corresponding to each rat and respective group. The histopathological analysis was conducted at the Instituto de Diagnóstico Anátomo Patológico (Institute of Pathological Anatomy Diagnostics) (IDAP), in the city of Florianópolis, by a single pathologist.

Bottom Line: Two animals had nonspecific spleen alterations.Conclusion.The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil.

ABSTRACT
Objective. To evaluate the possible migration of polymethylmethacrylate after injections in various corporal compartments of Wistar rats. Methods. The experimental work consisted in the injection of PMMA in corporal compartments for later histopathological analysis of the locations of implants and of distant filtering organs. The dose applied in each implant was of 0.2 mL. The animals were divided into groups according to the location of the implant realized: group GB had intradermic injections in the glabella. Group SD had subdermal injections in dorsal subcutaneous tissue cells. Group IP had intraperitoneal injections in the abdomen. Group PD had intramuscular injections in the right rear leg. The rats were sacrificed 30 days after realization of the implants and tissue samples from the lung, liver, spleen, and kidney, and locations of implantation were removed for histopathological analysis. Results. Characteristic microspheres that were compatible with the presence of PMMA in any of the histological slides analyzed were not observed. One animal had an amorphous exogenous substance, with a histiocytic reaction. Twelve of the 16 lungs analyzed had locations of intraalveolar hemorrhaging. Two animals had nonspecific spleen alterations. Conclusion. The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

No MeSH data available.


Related in: MedlinePlus