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Possible migration and histopathological analysis of injections of polymethylmethacrylate in wistar rats.

Neves Rd, Herdt MA, Wohlgemuth FB, Ely JB, de Vasconcellos ZA, Bastos JC, d'Acampora AJ - ISRN Dermatol (2012)

Bottom Line: Two animals had nonspecific spleen alterations.Conclusion.The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil.

ABSTRACT
Objective. To evaluate the possible migration of polymethylmethacrylate after injections in various corporal compartments of Wistar rats. Methods. The experimental work consisted in the injection of PMMA in corporal compartments for later histopathological analysis of the locations of implants and of distant filtering organs. The dose applied in each implant was of 0.2 mL. The animals were divided into groups according to the location of the implant realized: group GB had intradermic injections in the glabella. Group SD had subdermal injections in dorsal subcutaneous tissue cells. Group IP had intraperitoneal injections in the abdomen. Group PD had intramuscular injections in the right rear leg. The rats were sacrificed 30 days after realization of the implants and tissue samples from the lung, liver, spleen, and kidney, and locations of implantation were removed for histopathological analysis. Results. Characteristic microspheres that were compatible with the presence of PMMA in any of the histological slides analyzed were not observed. One animal had an amorphous exogenous substance, with a histiocytic reaction. Twelve of the 16 lungs analyzed had locations of intraalveolar hemorrhaging. Two animals had nonspecific spleen alterations. Conclusion. The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

No MeSH data available.


Related in: MedlinePlus

Subdermal application of PMMA in the dorsal (a), intraperitoneal in the right hemi-abdominal (b), and intramuscularly in the right membrum pelvinum (c).
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fig3: Subdermal application of PMMA in the dorsal (a), intraperitoneal in the right hemi-abdominal (b), and intramuscularly in the right membrum pelvinum (c).

Mentions: The SD subgroup included four animals which, after receiving anesthesia, were injected with 0.2 mL of PMMA in the cervical dorsal region on the subdermal plane (Figure 3(a)). These animals were submited to a painless induced death 30 days after the PMMA injection. For the histopathological procedure of this group, the skin and subcutaneous cellular tissue were removed from the implanted area of each animal.


Possible migration and histopathological analysis of injections of polymethylmethacrylate in wistar rats.

Neves Rd, Herdt MA, Wohlgemuth FB, Ely JB, de Vasconcellos ZA, Bastos JC, d'Acampora AJ - ISRN Dermatol (2012)

Subdermal application of PMMA in the dorsal (a), intraperitoneal in the right hemi-abdominal (b), and intramuscularly in the right membrum pelvinum (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369493&req=5

fig3: Subdermal application of PMMA in the dorsal (a), intraperitoneal in the right hemi-abdominal (b), and intramuscularly in the right membrum pelvinum (c).
Mentions: The SD subgroup included four animals which, after receiving anesthesia, were injected with 0.2 mL of PMMA in the cervical dorsal region on the subdermal plane (Figure 3(a)). These animals were submited to a painless induced death 30 days after the PMMA injection. For the histopathological procedure of this group, the skin and subcutaneous cellular tissue were removed from the implanted area of each animal.

Bottom Line: Two animals had nonspecific spleen alterations.Conclusion.The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil.

ABSTRACT
Objective. To evaluate the possible migration of polymethylmethacrylate after injections in various corporal compartments of Wistar rats. Methods. The experimental work consisted in the injection of PMMA in corporal compartments for later histopathological analysis of the locations of implants and of distant filtering organs. The dose applied in each implant was of 0.2 mL. The animals were divided into groups according to the location of the implant realized: group GB had intradermic injections in the glabella. Group SD had subdermal injections in dorsal subcutaneous tissue cells. Group IP had intraperitoneal injections in the abdomen. Group PD had intramuscular injections in the right rear leg. The rats were sacrificed 30 days after realization of the implants and tissue samples from the lung, liver, spleen, and kidney, and locations of implantation were removed for histopathological analysis. Results. Characteristic microspheres that were compatible with the presence of PMMA in any of the histological slides analyzed were not observed. One animal had an amorphous exogenous substance, with a histiocytic reaction. Twelve of the 16 lungs analyzed had locations of intraalveolar hemorrhaging. Two animals had nonspecific spleen alterations. Conclusion. The histopathological analysis of this study found no PMMA microspheres in any of the tissues analyzed.

No MeSH data available.


Related in: MedlinePlus