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The Importance of Definitive Diagnosis in Chronic Schistosomiasis, with Reference to Schistosoma haematobium.

Shiff C - J Parasitol Res (2012)

Bottom Line: Schistosomes are long-lived parasites, hence schistosomiasis is a chronic disease with severe long-term implications.Detection of parasite-specific DNA in urine has been demonstrated and this has similar specificity but improved sensitivity.The implications of this new procedure and the impact on diagnosis are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.

ABSTRACT
Schistosomes are long-lived parasites, hence schistosomiasis is a chronic disease with severe long-term implications. However, definitive diagnosis of active infection has been difficult because demonstration of infection has depended on detecting parasite eggs in urine and/or stool. In the case of Schistosoma haematobium which parasitizes the urinogenital system, this method has low sensitivity in adults. Detection of parasite-specific DNA in urine has been demonstrated and this has similar specificity but improved sensitivity. The implications of this new procedure and the impact on diagnosis are discussed.

No MeSH data available.


Related in: MedlinePlus

The proportion of Schistosoma haematobium cases diagnosed by demonstration of eggs or schistosome-specific DNA in the urine. 398 specimens were collected from villagers in western Nigeria where the parasite is endemic. Numbers in table = number examined,  P  values given where significant.
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Related In: Results  -  Collection


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fig1: The proportion of Schistosoma haematobium cases diagnosed by demonstration of eggs or schistosome-specific DNA in the urine. 398 specimens were collected from villagers in western Nigeria where the parasite is endemic. Numbers in table = number examined,  P  values given where significant.

Mentions: Analysis of the dataset described above (Figure 1) shows that the proportion of positive cases detected for each age group in the study was higher when Dra1 was detected by DNA amplification than when parasite eggs were observed for all age groups (P = 0.0005), although when stratified across age groups, significant differences were only seen in the 20–29- (P = 0.004) and 40–49- (P = 0.02) year-old age groups. The message from these studies is that if adult populations are examined for schistosome infection, if the only test applied is examination of urine for eggs, a significant number of people will be declared uninfected, yet they may yet be infected.


The Importance of Definitive Diagnosis in Chronic Schistosomiasis, with Reference to Schistosoma haematobium.

Shiff C - J Parasitol Res (2012)

The proportion of Schistosoma haematobium cases diagnosed by demonstration of eggs or schistosome-specific DNA in the urine. 398 specimens were collected from villagers in western Nigeria where the parasite is endemic. Numbers in table = number examined,  P  values given where significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369489&req=5

fig1: The proportion of Schistosoma haematobium cases diagnosed by demonstration of eggs or schistosome-specific DNA in the urine. 398 specimens were collected from villagers in western Nigeria where the parasite is endemic. Numbers in table = number examined,  P  values given where significant.
Mentions: Analysis of the dataset described above (Figure 1) shows that the proportion of positive cases detected for each age group in the study was higher when Dra1 was detected by DNA amplification than when parasite eggs were observed for all age groups (P = 0.0005), although when stratified across age groups, significant differences were only seen in the 20–29- (P = 0.004) and 40–49- (P = 0.02) year-old age groups. The message from these studies is that if adult populations are examined for schistosome infection, if the only test applied is examination of urine for eggs, a significant number of people will be declared uninfected, yet they may yet be infected.

Bottom Line: Schistosomes are long-lived parasites, hence schistosomiasis is a chronic disease with severe long-term implications.Detection of parasite-specific DNA in urine has been demonstrated and this has similar specificity but improved sensitivity.The implications of this new procedure and the impact on diagnosis are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.

ABSTRACT
Schistosomes are long-lived parasites, hence schistosomiasis is a chronic disease with severe long-term implications. However, definitive diagnosis of active infection has been difficult because demonstration of infection has depended on detecting parasite eggs in urine and/or stool. In the case of Schistosoma haematobium which parasitizes the urinogenital system, this method has low sensitivity in adults. Detection of parasite-specific DNA in urine has been demonstrated and this has similar specificity but improved sensitivity. The implications of this new procedure and the impact on diagnosis are discussed.

No MeSH data available.


Related in: MedlinePlus