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Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J - ISRN Neurol (2012)

Bottom Line: In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases.In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%).In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Anatomy & Histology F13, The University of Sydney, Sydney, NSW 2006, Australia.

ABSTRACT
We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

No MeSH data available.


Related in: MedlinePlus

Schematic diagrams of maps ((a), (c), (e), (g)) and photomicrographs ((b), (d), (f), (h)) of TH+ amacrine cells in the retinae of Saline ((a), (b)), Saline-NIr ((c), (d)), MPTP ((e), (f)), and MPTP-NIr ((g), (h)) groups of the Ac-Sim case (this case shown because it had the most change after MPTP treatment). The photomicrographs are of a midregion of superior temporal retina in each case. In the saline control groups ((a)–(d)), TH+ cells were distributed relatively uniformly across the retina, but with a slight concentration in superior and temporal retina. In the MPTP and MPTP-NIr groups ((e)–(h)), there was no particular region of retina that was affected particularly after MPTP (or NIr) treatment. Scale bar = 100 μm.
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fig3: Schematic diagrams of maps ((a), (c), (e), (g)) and photomicrographs ((b), (d), (f), (h)) of TH+ amacrine cells in the retinae of Saline ((a), (b)), Saline-NIr ((c), (d)), MPTP ((e), (f)), and MPTP-NIr ((g), (h)) groups of the Ac-Sim case (this case shown because it had the most change after MPTP treatment). The photomicrographs are of a midregion of superior temporal retina in each case. In the saline control groups ((a)–(d)), TH+ cells were distributed relatively uniformly across the retina, but with a slight concentration in superior and temporal retina. In the MPTP and MPTP-NIr groups ((e)–(h)), there was no particular region of retina that was affected particularly after MPTP (or NIr) treatment. Scale bar = 100 μm.

Mentions: We examined the distribution of TH+ cells in the different cases as to determine whether the MPTP or NIr treatment affected one retinal region more than another. Figure 3 shows maps and photomicrographs of TH+ cells in the representative retinae of Saline (Figures 3(a) and 3(b)), Saline-NIr (Figures 3(c) and 3(d)), MPTP (Figures 3(e) and 3(f), and MPTP-NIr (Figures 3(g) and 3(h) groups of the Ac-Sim case (this case shown because it had the most change after MPTP treatment). In all groups, TH+ cells were found in all retinal regions, but with more tendency to be located in superior and temporal retina [19, 21]. There was no particular region of retina that was affected greatly by either MPTP or NIr treatment, although there were fewer cells in the MPTP groups (see above in this case). In general, these patterns of distribution in each group were similar in the different cases.


Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J - ISRN Neurol (2012)

Schematic diagrams of maps ((a), (c), (e), (g)) and photomicrographs ((b), (d), (f), (h)) of TH+ amacrine cells in the retinae of Saline ((a), (b)), Saline-NIr ((c), (d)), MPTP ((e), (f)), and MPTP-NIr ((g), (h)) groups of the Ac-Sim case (this case shown because it had the most change after MPTP treatment). The photomicrographs are of a midregion of superior temporal retina in each case. In the saline control groups ((a)–(d)), TH+ cells were distributed relatively uniformly across the retina, but with a slight concentration in superior and temporal retina. In the MPTP and MPTP-NIr groups ((e)–(h)), there was no particular region of retina that was affected particularly after MPTP (or NIr) treatment. Scale bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Schematic diagrams of maps ((a), (c), (e), (g)) and photomicrographs ((b), (d), (f), (h)) of TH+ amacrine cells in the retinae of Saline ((a), (b)), Saline-NIr ((c), (d)), MPTP ((e), (f)), and MPTP-NIr ((g), (h)) groups of the Ac-Sim case (this case shown because it had the most change after MPTP treatment). The photomicrographs are of a midregion of superior temporal retina in each case. In the saline control groups ((a)–(d)), TH+ cells were distributed relatively uniformly across the retina, but with a slight concentration in superior and temporal retina. In the MPTP and MPTP-NIr groups ((e)–(h)), there was no particular region of retina that was affected particularly after MPTP (or NIr) treatment. Scale bar = 100 μm.
Mentions: We examined the distribution of TH+ cells in the different cases as to determine whether the MPTP or NIr treatment affected one retinal region more than another. Figure 3 shows maps and photomicrographs of TH+ cells in the representative retinae of Saline (Figures 3(a) and 3(b)), Saline-NIr (Figures 3(c) and 3(d)), MPTP (Figures 3(e) and 3(f), and MPTP-NIr (Figures 3(g) and 3(h) groups of the Ac-Sim case (this case shown because it had the most change after MPTP treatment). In all groups, TH+ cells were found in all retinal regions, but with more tendency to be located in superior and temporal retina [19, 21]. There was no particular region of retina that was affected greatly by either MPTP or NIr treatment, although there were fewer cells in the MPTP groups (see above in this case). In general, these patterns of distribution in each group were similar in the different cases.

Bottom Line: In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases.In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%).In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Anatomy & Histology F13, The University of Sydney, Sydney, NSW 2006, Australia.

ABSTRACT
We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

No MeSH data available.


Related in: MedlinePlus