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Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J - ISRN Neurol (2012)

Bottom Line: In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases.In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%).In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Anatomy & Histology F13, The University of Sydney, Sydney, NSW 2006, Australia.

ABSTRACT
We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

No MeSH data available.


Related in: MedlinePlus

Graphs showing (a) the areas and (b) the number of TH+ amacrine cells in the retinae in the four experimental groups in the different cases (different shaded columns). Columns show the mean ± standard error of the total number of cells in each case. †Represents P < 0.001, ∧represents P < 0.01 and *represents P < 0.05 significant difference in cell number; these symbols on the columns of the MPTP group represent differences from the Saline group of each case, while symbols on the MPTP-NIr group represent differences from the MPTP group of each case. There were clear increases in TH+ cell number in the MPTP-NIr group compared to the MPTP group in all cases, particularly the Ac-PT, Ch-Sim, and Ch-PT.
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Related In: Results  -  Collection


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fig2: Graphs showing (a) the areas and (b) the number of TH+ amacrine cells in the retinae in the four experimental groups in the different cases (different shaded columns). Columns show the mean ± standard error of the total number of cells in each case. †Represents P < 0.001, ∧represents P < 0.01 and *represents P < 0.05 significant difference in cell number; these symbols on the columns of the MPTP group represent differences from the Saline group of each case, while symbols on the MPTP-NIr group represent differences from the MPTP group of each case. There were clear increases in TH+ cell number in the MPTP-NIr group compared to the MPTP group in all cases, particularly the Ac-PT, Ch-Sim, and Ch-PT.

Mentions: The graph in Figure 2(a) shows the retinal areas in the different experimental groups in each of the Acute-Simultaneous (Ac-Sim), Acute-Posttreatment (Ac-PT), Chronic-Simultaneous (Ch-Sim), and Chronic-Posttreatment (Ch-PT) cases. Overall, these values were similar to those reported previously for the retinal area of mice (~15 mm2) [19]. We found no significant difference between the retinal areas of the different cases (ANOVA test: F = 1.1; P = 0.4), indicating that our MPTP or NIr treatment had no impact on retinal area.


Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J - ISRN Neurol (2012)

Graphs showing (a) the areas and (b) the number of TH+ amacrine cells in the retinae in the four experimental groups in the different cases (different shaded columns). Columns show the mean ± standard error of the total number of cells in each case. †Represents P < 0.001, ∧represents P < 0.01 and *represents P < 0.05 significant difference in cell number; these symbols on the columns of the MPTP group represent differences from the Saline group of each case, while symbols on the MPTP-NIr group represent differences from the MPTP group of each case. There were clear increases in TH+ cell number in the MPTP-NIr group compared to the MPTP group in all cases, particularly the Ac-PT, Ch-Sim, and Ch-PT.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369478&req=5

fig2: Graphs showing (a) the areas and (b) the number of TH+ amacrine cells in the retinae in the four experimental groups in the different cases (different shaded columns). Columns show the mean ± standard error of the total number of cells in each case. †Represents P < 0.001, ∧represents P < 0.01 and *represents P < 0.05 significant difference in cell number; these symbols on the columns of the MPTP group represent differences from the Saline group of each case, while symbols on the MPTP-NIr group represent differences from the MPTP group of each case. There were clear increases in TH+ cell number in the MPTP-NIr group compared to the MPTP group in all cases, particularly the Ac-PT, Ch-Sim, and Ch-PT.
Mentions: The graph in Figure 2(a) shows the retinal areas in the different experimental groups in each of the Acute-Simultaneous (Ac-Sim), Acute-Posttreatment (Ac-PT), Chronic-Simultaneous (Ch-Sim), and Chronic-Posttreatment (Ch-PT) cases. Overall, these values were similar to those reported previously for the retinal area of mice (~15 mm2) [19]. We found no significant difference between the retinal areas of the different cases (ANOVA test: F = 1.1; P = 0.4), indicating that our MPTP or NIr treatment had no impact on retinal area.

Bottom Line: In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases.In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%).In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Anatomy & Histology F13, The University of Sydney, Sydney, NSW 2006, Australia.

ABSTRACT
We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

No MeSH data available.


Related in: MedlinePlus