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Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J - ISRN Neurol (2012)

Bottom Line: In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases.In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%).In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Anatomy & Histology F13, The University of Sydney, Sydney, NSW 2006, Australia.

ABSTRACT
We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

No MeSH data available.


Related in: MedlinePlus

Outline of the different experimental groups used in this study, namely: Saline, Saline-NIr, MPTP, and MPTP-NIr, in either the simultaneous (a) or posttreatment (b) series of the acute and chronic models. Photomicrographs of TH+ amacrine cells in retinal wholemounts of the superior temporal region (c) and of the retinal edges ((d), (e)). The latter reveals the location of the TH+ somata and dendrites within the different layers. Most TH+ somata were located in the inner layers of the inner nuclear layer (c); very few were located in the ganglion cell layer (e). All images from Saline group of Ac-Sim case. Scale bars = 100 μm.
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fig1: Outline of the different experimental groups used in this study, namely: Saline, Saline-NIr, MPTP, and MPTP-NIr, in either the simultaneous (a) or posttreatment (b) series of the acute and chronic models. Photomicrographs of TH+ amacrine cells in retinal wholemounts of the superior temporal region (c) and of the retinal edges ((d), (e)). The latter reveals the location of the TH+ somata and dendrites within the different layers. Most TH+ somata were located in the inner layers of the inner nuclear layer (c); very few were located in the ganglion cell layer (e). All images from Saline group of Ac-Sim case. Scale bars = 100 μm.

Mentions: An acute [4, 6, 7] and a chronic [5, 8] MPTP models were used in this study (Figures 1(a) and 1(b)). In each, NIr treatment was applied either at approximately the same time (simultaneous) or well after (posttreatment) the MPTP insult (Figures 1(a) and 1(b)). Hence, each model had two series, simultaneous (Acute-Simultaneous [Ac-Sim], Chronic-Simultaneous [Ch-Sim]) and posttreatment (Acute-Posttreatment [Ac-PT], Chronic-Posttreatment [Ch-PT]). Within each of these, there were four experimental groups, where mice received intraperitoneal injections of either MPTP or saline, combined with NIr treatments or not (Figures 1(a) and 1(b)). The different groups were: (1) Saline (n = 21): saline injections with no NIr (2) Saline-NIr (n = 19): saline injections with NIr (3) MPTP (n = 22): MPTP injections with no NIr (4) MPTP-NIr (n = 18): MPTP injections with NIr.


Survival of Dopaminergic Amacrine Cells after Near-Infrared Light Treatment in MPTP-Treated Mice.

Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J - ISRN Neurol (2012)

Outline of the different experimental groups used in this study, namely: Saline, Saline-NIr, MPTP, and MPTP-NIr, in either the simultaneous (a) or posttreatment (b) series of the acute and chronic models. Photomicrographs of TH+ amacrine cells in retinal wholemounts of the superior temporal region (c) and of the retinal edges ((d), (e)). The latter reveals the location of the TH+ somata and dendrites within the different layers. Most TH+ somata were located in the inner layers of the inner nuclear layer (c); very few were located in the ganglion cell layer (e). All images from Saline group of Ac-Sim case. Scale bars = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369478&req=5

fig1: Outline of the different experimental groups used in this study, namely: Saline, Saline-NIr, MPTP, and MPTP-NIr, in either the simultaneous (a) or posttreatment (b) series of the acute and chronic models. Photomicrographs of TH+ amacrine cells in retinal wholemounts of the superior temporal region (c) and of the retinal edges ((d), (e)). The latter reveals the location of the TH+ somata and dendrites within the different layers. Most TH+ somata were located in the inner layers of the inner nuclear layer (c); very few were located in the ganglion cell layer (e). All images from Saline group of Ac-Sim case. Scale bars = 100 μm.
Mentions: An acute [4, 6, 7] and a chronic [5, 8] MPTP models were used in this study (Figures 1(a) and 1(b)). In each, NIr treatment was applied either at approximately the same time (simultaneous) or well after (posttreatment) the MPTP insult (Figures 1(a) and 1(b)). Hence, each model had two series, simultaneous (Acute-Simultaneous [Ac-Sim], Chronic-Simultaneous [Ch-Sim]) and posttreatment (Acute-Posttreatment [Ac-PT], Chronic-Posttreatment [Ch-PT]). Within each of these, there were four experimental groups, where mice received intraperitoneal injections of either MPTP or saline, combined with NIr treatments or not (Figures 1(a) and 1(b)). The different groups were: (1) Saline (n = 21): saline injections with no NIr (2) Saline-NIr (n = 19): saline injections with NIr (3) MPTP (n = 22): MPTP injections with no NIr (4) MPTP-NIr (n = 18): MPTP injections with NIr.

Bottom Line: In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases.In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%).In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Anatomy & Histology F13, The University of Sydney, Sydney, NSW 2006, Australia.

ABSTRACT
We examined whether near-infrared light (NIr) treatment (photobiomodulation) saves dopaminergic amacrine cells of the retina in an acute and a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease. For the acute model, BALB/c mice had MPTP (100 mg/kg) or saline injections over 30 hours, followed by a six-day-survival period. For the chronic model, mice had MPTP (200 mg/kg) or saline injections over five weeks, followed by a three-week-survival period. NIr treatment was applied either at the same time (simultaneous series) or well after (posttreatment series) the MPTP insult. There were four groups within each series: Saline, Saline-NIr, MPTP, and MPTP-NIr. Retinae were processed for tyrosine hydroxylase (TH) immunochemistry, and cell number was analysed. In the MPTP groups, there was a significant reduction in TH(+) cell number compared to the saline controls; this reduction was greater in the acute (~50%) compared to the chronic (~30%) cases. In the MPTP-NIr groups, there were significantly more TH(+) cells than in the MPTP groups of both series (~30%). In summary, we showed that NIr treatment was able to both protect (simultaneous series) and rescue (posttreatment series) TH(+) cells of the retina from parkinsonian insult.

No MeSH data available.


Related in: MedlinePlus