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Changes of gene expression after bone marrow cell transfusion in rats with monocrotaline-induced pulmonary hypertension.

Kim KC, Lee HR, Kim SJ, Cho MS, Hong YM - J. Korean Med. Sci. (2012)

Bottom Line: Results showed that the average RV pressure significantly decreased in the B group compared with the M group.RV weight and the ratio of RH/LH+septum significantly decreased in the B group compared to the M group.Gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α significantly decreased in week 4 in the B group compared with the M group.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Ewha Womans University, Seoul, Korea.

ABSTRACT
Pulmonary artery hypertension (PAH) causes right ventricular failure and possibly even death by a progressive increase in pulmonary vascular resistance. Bone marrow-derived mesenchymal stem cell therapy has provided an alternative treatment for ailments of various organs by promoting cell regeneration at the site of pathology. The purpose of this study was to investigate changes of pulmonary haemodynamics, pathology and expressions of various genes, including ET (endothelin)-1, ET receptor A (ERA), endothelial nitric oxide synthase (NOS) 3, matrix metalloproteinase (MMP) 2, tissue inhibitor of matrix metalloproteinase (TIMP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α in monocrotaline (MCT)-induced PAH rat models after bone marrow cell (BMC) transfusion. The rats were grouped as the control (C) group, monocrotaline (M) group, and BMC transfusion (B) group. M and B groups received subcutaneous (sc) injection of MCT (60 mg/kg). BMCs were transfused by intravenous injection at the tail 1 week after MCT injection in B group. Results showed that the average RV pressure significantly decreased in the B group compared with the M group. RV weight and the ratio of RH/LH+septum significantly decreased in the B group compared to the M group. Gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α significantly decreased in week 4 in the B group compared with the M group. In conclusion, BMC transfusion appears to improve survival rate, RVH, and mean RV pressure, and decreases gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α.

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The RT-PCR products from the transcripts of IL-6, TNF-α, ET-1, ERA, NOS3, MMP2, TIMP and TIMP were 132 bp, 113 bp, 156 bp, 118 bp, 140 bp, 137 bp, and 133 bp respectively. TNF, tumor necrosis factor; ET-1, endothelin-1; ERA, endothelin receptor A; NOS-3, endothelial nitric oxide synthase; MMP 2, matrix metalloproteinase 2; TIMP, tissue inhibitor of matrix metalloproteinases; IL, interleukin.
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Figure 2: The RT-PCR products from the transcripts of IL-6, TNF-α, ET-1, ERA, NOS3, MMP2, TIMP and TIMP were 132 bp, 113 bp, 156 bp, 118 bp, 140 bp, 137 bp, and 133 bp respectively. TNF, tumor necrosis factor; ET-1, endothelin-1; ERA, endothelin receptor A; NOS-3, endothelial nitric oxide synthase; MMP 2, matrix metalloproteinase 2; TIMP, tissue inhibitor of matrix metalloproteinases; IL, interleukin.

Mentions: The specific primers for TNF-α were 5'-GAC AGT TGA TTT CTG GGC CCT TT-3' (forward) and 5'-CCA CTG TTC TGT GCT CC-3' (reverse) (Fig. 2).


Changes of gene expression after bone marrow cell transfusion in rats with monocrotaline-induced pulmonary hypertension.

Kim KC, Lee HR, Kim SJ, Cho MS, Hong YM - J. Korean Med. Sci. (2012)

The RT-PCR products from the transcripts of IL-6, TNF-α, ET-1, ERA, NOS3, MMP2, TIMP and TIMP were 132 bp, 113 bp, 156 bp, 118 bp, 140 bp, 137 bp, and 133 bp respectively. TNF, tumor necrosis factor; ET-1, endothelin-1; ERA, endothelin receptor A; NOS-3, endothelial nitric oxide synthase; MMP 2, matrix metalloproteinase 2; TIMP, tissue inhibitor of matrix metalloproteinases; IL, interleukin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369445&req=5

Figure 2: The RT-PCR products from the transcripts of IL-6, TNF-α, ET-1, ERA, NOS3, MMP2, TIMP and TIMP were 132 bp, 113 bp, 156 bp, 118 bp, 140 bp, 137 bp, and 133 bp respectively. TNF, tumor necrosis factor; ET-1, endothelin-1; ERA, endothelin receptor A; NOS-3, endothelial nitric oxide synthase; MMP 2, matrix metalloproteinase 2; TIMP, tissue inhibitor of matrix metalloproteinases; IL, interleukin.
Mentions: The specific primers for TNF-α were 5'-GAC AGT TGA TTT CTG GGC CCT TT-3' (forward) and 5'-CCA CTG TTC TGT GCT CC-3' (reverse) (Fig. 2).

Bottom Line: Results showed that the average RV pressure significantly decreased in the B group compared with the M group.RV weight and the ratio of RH/LH+septum significantly decreased in the B group compared to the M group.Gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α significantly decreased in week 4 in the B group compared with the M group.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Ewha Womans University, Seoul, Korea.

ABSTRACT
Pulmonary artery hypertension (PAH) causes right ventricular failure and possibly even death by a progressive increase in pulmonary vascular resistance. Bone marrow-derived mesenchymal stem cell therapy has provided an alternative treatment for ailments of various organs by promoting cell regeneration at the site of pathology. The purpose of this study was to investigate changes of pulmonary haemodynamics, pathology and expressions of various genes, including ET (endothelin)-1, ET receptor A (ERA), endothelial nitric oxide synthase (NOS) 3, matrix metalloproteinase (MMP) 2, tissue inhibitor of matrix metalloproteinase (TIMP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α in monocrotaline (MCT)-induced PAH rat models after bone marrow cell (BMC) transfusion. The rats were grouped as the control (C) group, monocrotaline (M) group, and BMC transfusion (B) group. M and B groups received subcutaneous (sc) injection of MCT (60 mg/kg). BMCs were transfused by intravenous injection at the tail 1 week after MCT injection in B group. Results showed that the average RV pressure significantly decreased in the B group compared with the M group. RV weight and the ratio of RH/LH+septum significantly decreased in the B group compared to the M group. Gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α significantly decreased in week 4 in the B group compared with the M group. In conclusion, BMC transfusion appears to improve survival rate, RVH, and mean RV pressure, and decreases gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α.

Show MeSH
Related in: MedlinePlus