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Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

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Open field locomotor assessment using Basso-Beattie-Bresnahan scale tested at every week after SCI. All subjects show a gradual improvement in hindlimb function during the 6 week observation period. Stastistical analysis indicated that BBB scales in the MSCs transplanted group were significantly higher than those in the control group (n = 8, P < 0.05). The rats in the IV group show the highest improvement at the last follow-up.
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Figure 6: Open field locomotor assessment using Basso-Beattie-Bresnahan scale tested at every week after SCI. All subjects show a gradual improvement in hindlimb function during the 6 week observation period. Stastistical analysis indicated that BBB scales in the MSCs transplanted group were significantly higher than those in the control group (n = 8, P < 0.05). The rats in the IV group show the highest improvement at the last follow-up.

Mentions: All the injured rats manifested complete hindlimb paraplegia immediately after the operation. In all the groups, the rats gradually recovered varying degrees of motor function over the time of observation (Fig. 6). At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. The functional recovery seen in the rats that underwent MSCs transplantation was significantly better than that in the control group (P < 0.05).


Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Open field locomotor assessment using Basso-Beattie-Bresnahan scale tested at every week after SCI. All subjects show a gradual improvement in hindlimb function during the 6 week observation period. Stastistical analysis indicated that BBB scales in the MSCs transplanted group were significantly higher than those in the control group (n = 8, P < 0.05). The rats in the IV group show the highest improvement at the last follow-up.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369443&req=5

Figure 6: Open field locomotor assessment using Basso-Beattie-Bresnahan scale tested at every week after SCI. All subjects show a gradual improvement in hindlimb function during the 6 week observation period. Stastistical analysis indicated that BBB scales in the MSCs transplanted group were significantly higher than those in the control group (n = 8, P < 0.05). The rats in the IV group show the highest improvement at the last follow-up.
Mentions: All the injured rats manifested complete hindlimb paraplegia immediately after the operation. In all the groups, the rats gradually recovered varying degrees of motor function over the time of observation (Fig. 6). At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. The functional recovery seen in the rats that underwent MSCs transplantation was significantly better than that in the control group (P < 0.05).

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

Show MeSH
Related in: MedlinePlus