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Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

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Expression of NGF. Post-injury 1 week NGF levels in the spinal cord tissues were measured. The relative optical densities in the control, IV and IL groups are 2.16 ± 0.04, 1.70 ± 0.23, 2.41 ± 0.15 respectively. The IL group shows significantly higher level compared to those in the control and IV groups. And there is also significant difference between the groups (n = 4, P < 0.05).
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Figure 5: Expression of NGF. Post-injury 1 week NGF levels in the spinal cord tissues were measured. The relative optical densities in the control, IV and IL groups are 2.16 ± 0.04, 1.70 ± 0.23, 2.41 ± 0.15 respectively. The IL group shows significantly higher level compared to those in the control and IV groups. And there is also significant difference between the groups (n = 4, P < 0.05).

Mentions: BDNF and NGF levels in the spinal cord tissue (n = 4, each group) were measured. BDNF level in the IL group (mean relative optical density, 1.70 ± 0.2) were slightly increased compared to those in the control group (1.58 ± 0.22) and the IV group (1.39 ± 0.35). However, there was no statistical significance (Fig. 4). NGF level in the IL group (mean relative optical density, 2.4 ± 0.15) was significantly increased compared to the control (2.16 ± 0.04) and the IV group (1.7 ± 0.23) (P < 0.05) (Fig. 5).


Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Expression of NGF. Post-injury 1 week NGF levels in the spinal cord tissues were measured. The relative optical densities in the control, IV and IL groups are 2.16 ± 0.04, 1.70 ± 0.23, 2.41 ± 0.15 respectively. The IL group shows significantly higher level compared to those in the control and IV groups. And there is also significant difference between the groups (n = 4, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369443&req=5

Figure 5: Expression of NGF. Post-injury 1 week NGF levels in the spinal cord tissues were measured. The relative optical densities in the control, IV and IL groups are 2.16 ± 0.04, 1.70 ± 0.23, 2.41 ± 0.15 respectively. The IL group shows significantly higher level compared to those in the control and IV groups. And there is also significant difference between the groups (n = 4, P < 0.05).
Mentions: BDNF and NGF levels in the spinal cord tissue (n = 4, each group) were measured. BDNF level in the IL group (mean relative optical density, 1.70 ± 0.2) were slightly increased compared to those in the control group (1.58 ± 0.22) and the IV group (1.39 ± 0.35). However, there was no statistical significance (Fig. 4). NGF level in the IL group (mean relative optical density, 2.4 ± 0.15) was significantly increased compared to the control (2.16 ± 0.04) and the IV group (1.7 ± 0.23) (P < 0.05) (Fig. 5).

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

Show MeSH
Related in: MedlinePlus