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Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

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Type II collagen expression of the transplanted cells were observed. In both transplanted groups, cells with a colocalization of PKH 26 and collagen II were not detected. (A) The IV transplanted MSCs do not express type II collagen. (B) Some type II collagen expression are noted in the IL group, however, no colocalization are found with PKH26 expression (collagen was tagged with green fluorescence, magnification × 400, scale bar 20 µm).
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Figure 2: Type II collagen expression of the transplanted cells were observed. In both transplanted groups, cells with a colocalization of PKH 26 and collagen II were not detected. (A) The IV transplanted MSCs do not express type II collagen. (B) Some type II collagen expression are noted in the IL group, however, no colocalization are found with PKH26 expression (collagen was tagged with green fluorescence, magnification × 400, scale bar 20 µm).

Mentions: To identify an aberrant differentiation of the transplanted cells to mesenchymal lineage, type II collagen staining was performed. Primary antibody to type II collagen (1:100, Lab Vision, Fremont, CA, USA) was used. Alexa Fluor 594 was used for immunofluorescence detection of collagen. Neither of the transplanted groups showed collagen expressing transplanted cells (Fig. 2).


Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Type II collagen expression of the transplanted cells were observed. In both transplanted groups, cells with a colocalization of PKH 26 and collagen II were not detected. (A) The IV transplanted MSCs do not express type II collagen. (B) Some type II collagen expression are noted in the IL group, however, no colocalization are found with PKH26 expression (collagen was tagged with green fluorescence, magnification × 400, scale bar 20 µm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369443&req=5

Figure 2: Type II collagen expression of the transplanted cells were observed. In both transplanted groups, cells with a colocalization of PKH 26 and collagen II were not detected. (A) The IV transplanted MSCs do not express type II collagen. (B) Some type II collagen expression are noted in the IL group, however, no colocalization are found with PKH26 expression (collagen was tagged with green fluorescence, magnification × 400, scale bar 20 µm).
Mentions: To identify an aberrant differentiation of the transplanted cells to mesenchymal lineage, type II collagen staining was performed. Primary antibody to type II collagen (1:100, Lab Vision, Fremont, CA, USA) was used. Alexa Fluor 594 was used for immunofluorescence detection of collagen. Neither of the transplanted groups showed collagen expressing transplanted cells (Fig. 2).

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

Show MeSH
Related in: MedlinePlus