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Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

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Flow cytomertric analysis of cultured cells with CD 45, 73, and 29. The positive expression of CD 73 and CD 29, and negative expression of CD 45 indicate its mesenchymal stem cell lineage.
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Figure 1: Flow cytomertric analysis of cultured cells with CD 45, 73, and 29. The positive expression of CD 73 and CD 29, and negative expression of CD 45 indicate its mesenchymal stem cell lineage.

Mentions: Before transplantation, the third passage cells were evaluated to confirm their phenotype as MSCs. Flow cytometry analysis of the MSCs showed positive cell surface markers in CD 29 and CD 73. The cultured MSCs lacked the expression of CD 45 (Fig. 1). This represents that the cultured cells have characteristics of MSCs.


Fate of transplanted bone marrow derived mesenchymal stem cells following spinal cord injury in rats by transplantation routes.

Kang ES, Ha KY, Kim YH - J. Korean Med. Sci. (2012)

Flow cytomertric analysis of cultured cells with CD 45, 73, and 29. The positive expression of CD 73 and CD 29, and negative expression of CD 45 indicate its mesenchymal stem cell lineage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369443&req=5

Figure 1: Flow cytomertric analysis of cultured cells with CD 45, 73, and 29. The positive expression of CD 73 and CD 29, and negative expression of CD 45 indicate its mesenchymal stem cell lineage.
Mentions: Before transplantation, the third passage cells were evaluated to confirm their phenotype as MSCs. Flow cytometry analysis of the MSCs showed positive cell surface markers in CD 29 and CD 73. The cultured MSCs lacked the expression of CD 45 (Fig. 1). This represents that the cultured cells have characteristics of MSCs.

Bottom Line: Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group.The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23).However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Seoul St. Mary's Hosptial, College of Medicine, The Catholic University of Korea, Seoul, Korea.

ABSTRACT
This research was performed to investigate the differences of the transplanted cells' survival and differentiation, and its efficacy according to the delivery routes following spinal cord injury. Allogenic mesenchymal stem cells (MSCs) were transplanted intravenously (IV group) or intralesionally (IL group) at post-injury 1 day in rats. Behavioral improvement, engraftment and differentiation of the transplanted cells and the expression of neurotrophic factors of the transplanted groups were analyzed and compared with those of the control group. At 6 weeks post-injury, the mean BBB motor scales in the control, IV and IL groups were 6.5 ± 1.8, 11.1 ± 2.1, and 8.5 ± 2.8, respectively. Regardless of the delivery route, the MSCs transplantation following spinal cord injuries presented better behavioral improvement. The differentiations of the engrafted cells were different according to the delivery routes. The engrafted cells predominantly differentiated into astrocytes in the IV group and on the other hand, engrafted cells of the IL group demonstrated relatively even neural and glial differentiation. The expressions of neuronal growth factor were significantly higher in the IL group (mean relative optical density, 2.4 ± 0.15) than those in the control (2.16 ± 0.04) or IV group (1.7 ± 0.23). Transplantation of MSCs in the early stage of spinal cord injury gives a significant clinical improvement. However, the fate of the transplanted MSCs and expression of neuronal growth factors are different along the transplantation route.

Show MeSH
Related in: MedlinePlus