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Classification of Traditional Chinese Medicine Syndromes in Patients with Chronic Hepatitis B by SELDI-Based ProteinChip Analysis.

Song YN, Zhang H, Guan Y, Peng JH, Lu YY, Hu YY, Su SB - Evid Based Complement Alternat Med (2012)

Bottom Line: Based on SELDI ProteinChip data, healthy controls and CHB patients or excess and deficiency syndromes in CHB patients were obviously differentiated by orthogonal partial least square (OPLS) analysis.Moreover, the area under the receiver operating characteristic (ROC) curve was 0.887 for classifying excess and nonexcess syndrome, and 0.700 for classifying deficiency and nondeficiency syndrome, respectively.Therefore, the present study provided the possibility of TCM syndrome classification in CHB patients using a universally acceptable scientific approach.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine,1200 Cailun Road, Pudong, Shanghai 201203, China.

ABSTRACT
Traditional Chinese medicine (TCM) syndrome, also called ZHENG, is the basis concept of TCM theory. It plays an important role in TCM practice. There are excess and deficiency syndromes in TCM syndrome. They are the common syndromes in chronic hepatitis B (CHB) patients. Here we aim to explore serum protein profiles and potential biomarkers for classification of TCM syndromes in CHB patients. 24 healthy controls and two cohorts of CHB patients of excess syndrome (n = 25) or deficiency syndrome (n = 19) were involved in this study. Protein profiles were obtained by surface-enhanced laser desorption ionization time-flight mass spectrometry (SELDI-TOF/MS) and multiple analyses were performed. Based on SELDI ProteinChip data, healthy controls and CHB patients or excess and deficiency syndromes in CHB patients were obviously differentiated by orthogonal partial least square (OPLS) analysis. Two significant serum proteins (m/z 4187 and m/z 5032) for classifying excess and deficiency syndromes were found. Moreover, the area under the receiver operating characteristic (ROC) curve was 0.887 for classifying excess and nonexcess syndrome, and 0.700 for classifying deficiency and nondeficiency syndrome, respectively. Therefore, the present study provided the possibility of TCM syndrome classification in CHB patients using a universally acceptable scientific approach.

No MeSH data available.


Related in: MedlinePlus

Box-plots for protein peak comparison between TCM syndrome groups. Proteins m/z 1216 (a), m/z 3168 (b), and m/z 4187 (c) were in lower abundance in excess syndrome group than those in deficiency syndrome one, while protein m/z 5032 (d) was in higher abundance.
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fig3: Box-plots for protein peak comparison between TCM syndrome groups. Proteins m/z 1216 (a), m/z 3168 (b), and m/z 4187 (c) were in lower abundance in excess syndrome group than those in deficiency syndrome one, while protein m/z 5032 (d) was in higher abundance.

Mentions: Among a total of 184 protein peaks detected, 4 significantly different peaks were observed between excess and deficiency syndromes according to Wilcoxon rank-sum test. Three of four protein peaks were in lower abundance in excess syndrome group (Figures 3(a), 3(b), and 3(c)), and the remaining one was in higher abundance (Figure 3(d)). These statistically significant differences can be displayed clearly in the box-plots. Also, an enlarged view of m/z 3168 and m/z 4187 is shown in Figure 1(b). So they may be potential biomarkers for classifying excess syndrome and deficiency syndrome with CHB.


Classification of Traditional Chinese Medicine Syndromes in Patients with Chronic Hepatitis B by SELDI-Based ProteinChip Analysis.

Song YN, Zhang H, Guan Y, Peng JH, Lu YY, Hu YY, Su SB - Evid Based Complement Alternat Med (2012)

Box-plots for protein peak comparison between TCM syndrome groups. Proteins m/z 1216 (a), m/z 3168 (b), and m/z 4187 (c) were in lower abundance in excess syndrome group than those in deficiency syndrome one, while protein m/z 5032 (d) was in higher abundance.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369440&req=5

fig3: Box-plots for protein peak comparison between TCM syndrome groups. Proteins m/z 1216 (a), m/z 3168 (b), and m/z 4187 (c) were in lower abundance in excess syndrome group than those in deficiency syndrome one, while protein m/z 5032 (d) was in higher abundance.
Mentions: Among a total of 184 protein peaks detected, 4 significantly different peaks were observed between excess and deficiency syndromes according to Wilcoxon rank-sum test. Three of four protein peaks were in lower abundance in excess syndrome group (Figures 3(a), 3(b), and 3(c)), and the remaining one was in higher abundance (Figure 3(d)). These statistically significant differences can be displayed clearly in the box-plots. Also, an enlarged view of m/z 3168 and m/z 4187 is shown in Figure 1(b). So they may be potential biomarkers for classifying excess syndrome and deficiency syndrome with CHB.

Bottom Line: Based on SELDI ProteinChip data, healthy controls and CHB patients or excess and deficiency syndromes in CHB patients were obviously differentiated by orthogonal partial least square (OPLS) analysis.Moreover, the area under the receiver operating characteristic (ROC) curve was 0.887 for classifying excess and nonexcess syndrome, and 0.700 for classifying deficiency and nondeficiency syndrome, respectively.Therefore, the present study provided the possibility of TCM syndrome classification in CHB patients using a universally acceptable scientific approach.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine,1200 Cailun Road, Pudong, Shanghai 201203, China.

ABSTRACT
Traditional Chinese medicine (TCM) syndrome, also called ZHENG, is the basis concept of TCM theory. It plays an important role in TCM practice. There are excess and deficiency syndromes in TCM syndrome. They are the common syndromes in chronic hepatitis B (CHB) patients. Here we aim to explore serum protein profiles and potential biomarkers for classification of TCM syndromes in CHB patients. 24 healthy controls and two cohorts of CHB patients of excess syndrome (n = 25) or deficiency syndrome (n = 19) were involved in this study. Protein profiles were obtained by surface-enhanced laser desorption ionization time-flight mass spectrometry (SELDI-TOF/MS) and multiple analyses were performed. Based on SELDI ProteinChip data, healthy controls and CHB patients or excess and deficiency syndromes in CHB patients were obviously differentiated by orthogonal partial least square (OPLS) analysis. Two significant serum proteins (m/z 4187 and m/z 5032) for classifying excess and deficiency syndromes were found. Moreover, the area under the receiver operating characteristic (ROC) curve was 0.887 for classifying excess and nonexcess syndrome, and 0.700 for classifying deficiency and nondeficiency syndrome, respectively. Therefore, the present study provided the possibility of TCM syndrome classification in CHB patients using a universally acceptable scientific approach.

No MeSH data available.


Related in: MedlinePlus