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Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats.

Liu JH, Wu ZF, Sun J, Jiang L, Jiang S, Fu WB - Evid Based Complement Alternat Med (2012)

Bottom Line: Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression.The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine.Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China.

ABSTRACT
Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-induced depression model rats, and AC-cAMP-PKA signal transduction pathway is crucial for it.

No MeSH data available.


Related in: MedlinePlus

Effect of EA or Fluoxetine on AC transformation ratio, cAMP level, and PKA activity. *P < 0.05 versus control group; ##P < 0.01 versus CMS group; SP < 0.05 versus Fluoxetine group.
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fig6: Effect of EA or Fluoxetine on AC transformation ratio, cAMP level, and PKA activity. *P < 0.05 versus control group; ##P < 0.01 versus CMS group; SP < 0.05 versus Fluoxetine group.

Mentions: CMS produced a significant decrease in the ratio of AC transformation compared with the control group (0.44 ± 0.07 versus 1.08 ± 0.15, P < 0.01), which was reversed by EA or Fluoxetine treatment (1.08 ± 0.07, 0.86 ± 0.17, P < 0.01, P < 0.01). Changes in cAMP level and PKA activity were similar to AC (Figure 6).


Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats.

Liu JH, Wu ZF, Sun J, Jiang L, Jiang S, Fu WB - Evid Based Complement Alternat Med (2012)

Effect of EA or Fluoxetine on AC transformation ratio, cAMP level, and PKA activity. *P < 0.05 versus control group; ##P < 0.01 versus CMS group; SP < 0.05 versus Fluoxetine group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369237&req=5

fig6: Effect of EA or Fluoxetine on AC transformation ratio, cAMP level, and PKA activity. *P < 0.05 versus control group; ##P < 0.01 versus CMS group; SP < 0.05 versus Fluoxetine group.
Mentions: CMS produced a significant decrease in the ratio of AC transformation compared with the control group (0.44 ± 0.07 versus 1.08 ± 0.15, P < 0.01), which was reversed by EA or Fluoxetine treatment (1.08 ± 0.07, 0.86 ± 0.17, P < 0.01, P < 0.01). Changes in cAMP level and PKA activity were similar to AC (Figure 6).

Bottom Line: Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression.The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine.Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China.

ABSTRACT
Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-induced depression model rats, and AC-cAMP-PKA signal transduction pathway is crucial for it.

No MeSH data available.


Related in: MedlinePlus