Limits...
Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats.

Liu JH, Wu ZF, Sun J, Jiang L, Jiang S, Fu WB - Evid Based Complement Alternat Med (2012)

Bottom Line: Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression.The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine.Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China.

ABSTRACT
Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-induced depression model rats, and AC-cAMP-PKA signal transduction pathway is crucial for it.

No MeSH data available.


Related in: MedlinePlus

Effect of EA or Fluoxetine on sucrose intake.  *P < 0.05, and **P < 0.01 versus control group, respectively;  −P < 0.05 versus EA + H89 group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3369237&req=5

fig4: Effect of EA or Fluoxetine on sucrose intake.  *P < 0.05, and **P < 0.01 versus control group, respectively;  −P < 0.05 versus EA + H89 group.

Mentions: Sucrose intake in all stress groups decreased significantly in the 3rd week and was much less than the control group (P < 0.05 or P < 0.01). However, there was no remarkable difference among all stress groups. EA (18.09 ± 1.90) or Fluoxetine (18.51 ± 1.30) treatment leaded to increase in sucrose intake in the sixth week, however, not indicating significant difference with CMS group (14.93 ± 1.83). H89 pretreatment inhibited obviously increase in sucrose intake induced by EA (12.99 ± 1.45 versus 18.09 ± 1.90, P < 0.05), but had no effect on the Fluoxetine (17.25 ± 1.61 versus 18.51 ± 1.30, P > 0.05) (Figure 4).


Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats.

Liu JH, Wu ZF, Sun J, Jiang L, Jiang S, Fu WB - Evid Based Complement Alternat Med (2012)

Effect of EA or Fluoxetine on sucrose intake.  *P < 0.05, and **P < 0.01 versus control group, respectively;  −P < 0.05 versus EA + H89 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369237&req=5

fig4: Effect of EA or Fluoxetine on sucrose intake.  *P < 0.05, and **P < 0.01 versus control group, respectively;  −P < 0.05 versus EA + H89 group.
Mentions: Sucrose intake in all stress groups decreased significantly in the 3rd week and was much less than the control group (P < 0.05 or P < 0.01). However, there was no remarkable difference among all stress groups. EA (18.09 ± 1.90) or Fluoxetine (18.51 ± 1.30) treatment leaded to increase in sucrose intake in the sixth week, however, not indicating significant difference with CMS group (14.93 ± 1.83). H89 pretreatment inhibited obviously increase in sucrose intake induced by EA (12.99 ± 1.45 versus 18.09 ± 1.90, P < 0.05), but had no effect on the Fluoxetine (17.25 ± 1.61 versus 18.51 ± 1.30, P > 0.05) (Figure 4).

Bottom Line: Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression.The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine.Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China.

ABSTRACT
Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-induced depression model rats, and AC-cAMP-PKA signal transduction pathway is crucial for it.

No MeSH data available.


Related in: MedlinePlus