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Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats.

Liu JH, Wu ZF, Sun J, Jiang L, Jiang S, Fu WB - Evid Based Complement Alternat Med (2012)

Bottom Line: Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression.The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine.Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China.

ABSTRACT
Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-induced depression model rats, and AC-cAMP-PKA signal transduction pathway is crucial for it.

No MeSH data available.


Related in: MedlinePlus

Experimental procedure.
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Related In: Results  -  Collection


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fig1: Experimental procedure.

Mentions: Before stress, all rats were screened out by the openfield test in which the total scores between 70 and 120 for all experiments and then were randomly divided into the following four groups (n = 8 per group): (1) control group (normal control with no stress); (2) CMS group (only received stress); (3) CMS plus EA group (received stress and EA); (4) fluoxetine group (received stress and Fluoxetine). To identify the role of cAMP cascade in the antidepression effect of EA treatment, a further 24 rats (8 in each group) were divided into three groups: (5) normal saline (NS) plus EA group; (6) H89 plus EA group; (7) H89 plus Fluoxetine group. Rats in the control group did not receive any stimulation. In the CMS group, rats were exposed to chronic mild unpredictable mild stress for 6 weeks. In the EA group, rats receive CMS treatment and EA stimulation at Siguan points (bilateral Hegu (LI4) and Taichong (LR3)) once every other day for 3 weeks after CMS exposure. In the Fluoxetine group, rats receive the same treatment as the CMS group and Fluoxetine treatment everyday for 3 weeks after CMS. The groups plus H89 were conditioned with the same as the EA or Fluoxetine group except for the intracerebroventricular injection of H89 between CMS and EA/Fluoxetine delivery. The design of the experiment was shown in Figure 1.


Role of AC-cAMP-PKA Cascade in Antidepressant Action of Electroacupuncture Treatment in Rats.

Liu JH, Wu ZF, Sun J, Jiang L, Jiang S, Fu WB - Evid Based Complement Alternat Med (2012)

Experimental procedure.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369237&req=5

fig1: Experimental procedure.
Mentions: Before stress, all rats were screened out by the openfield test in which the total scores between 70 and 120 for all experiments and then were randomly divided into the following four groups (n = 8 per group): (1) control group (normal control with no stress); (2) CMS group (only received stress); (3) CMS plus EA group (received stress and EA); (4) fluoxetine group (received stress and Fluoxetine). To identify the role of cAMP cascade in the antidepression effect of EA treatment, a further 24 rats (8 in each group) were divided into three groups: (5) normal saline (NS) plus EA group; (6) H89 plus EA group; (7) H89 plus Fluoxetine group. Rats in the control group did not receive any stimulation. In the CMS group, rats were exposed to chronic mild unpredictable mild stress for 6 weeks. In the EA group, rats receive CMS treatment and EA stimulation at Siguan points (bilateral Hegu (LI4) and Taichong (LR3)) once every other day for 3 weeks after CMS exposure. In the Fluoxetine group, rats receive the same treatment as the CMS group and Fluoxetine treatment everyday for 3 weeks after CMS. The groups plus H89 were conditioned with the same as the EA or Fluoxetine group except for the intracerebroventricular injection of H89 between CMS and EA/Fluoxetine delivery. The design of the experiment was shown in Figure 1.

Bottom Line: Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression.The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine.Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China.

ABSTRACT
Adenylyl cyclase (AC)-cyclic adenosine monophosphate (cAMP)-cAMP-dependent protein kinase A (PKA) cascade is considered to be associated with the pathogenesis and treatment of depression. The present study was conducted to explore the role of the cAMP cascade in antidepressant action of electroacupuncture (EA) treatment for chronic mild stress (CMS)-induced depression model rats. The results showed that EA improved significantly behavior symptoms in depression and dysfunction of AC-cAMP-PKA signal transduction pathway induced by CMS, which was as effective as fluoxetine. Moreover, the antidepressant effects of EA rather than Fluoxetine were completely abolished by H89, a specific PKA inhibitor. Consequently, EA has a significant antidepressant treatment in CMS-induced depression model rats, and AC-cAMP-PKA signal transduction pathway is crucial for it.

No MeSH data available.


Related in: MedlinePlus