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Controlled human wood smoke exposure: oxidative stress, inflammation and microvascular function.

Forchhammer L, Møller P, Riddervold IS, Bønløkke J, Massling A, Sigsgaard T, Loft S - Part Fibre Toxicol (2012)

Bottom Line: The MVF score was unaltered after inhalation of clean air (1.58 ± 0.07; mean ± SEM), low (1.51 ± 0.07) or high (1.61 ± 0.09) concentrations of wood smoke particles in atopic subjects, whereas unexposed non-atopic subjects had higher score (1.91 ± 0.09).The level of oxidatively damaged DNA, mRNA of ITGAL, CCL2, TNF, IL6, IL8, HMOX1, and OGG1 and surface marker molecules ICAM1, ITGAL and L-selectin in peripheral blood mononuclear cells were not affected by inhalation of wood smoke particles.Exposure to wood smoke had no effect on markers of oxidative stress, DNA damage, cell adhesion, cytokines or MVF in atopic subjects.

View Article: PubMed Central - HTML - PubMed

Affiliation: Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT

Background: Exposure to wood smoke is associated with respiratory symptoms, whereas knowledge on systemic effects is limited. We investigated effects on systemic inflammation, oxidative stress and microvascular function (MVF) after controlled wood smoke exposure.

Methods: In a randomised, double-blinded, cross-over study 20 non-smoking atopic subjects were exposed at rest to 14, 220, or 354 μg/m3 of particles from a well-burning modern wood stove for 3 h in a climate controlled chamber with 2 week intervals. We investigated the level of oxidatively damaged DNA, inflammatory markers and adhesion molecules before and 0, 6 and 20 h after exposure. Six h after exposure we measured MVF non-invasively by digital peripheral artery tonometry following arm ischemia.

Results: The MVF score was unaltered after inhalation of clean air (1.58 ± 0.07; mean ± SEM), low (1.51 ± 0.07) or high (1.61 ± 0.09) concentrations of wood smoke particles in atopic subjects, whereas unexposed non-atopic subjects had higher score (1.91 ± 0.09). The level of oxidatively damaged DNA, mRNA of ITGAL, CCL2, TNF, IL6, IL8, HMOX1, and OGG1 and surface marker molecules ICAM1, ITGAL and L-selectin in peripheral blood mononuclear cells were not affected by inhalation of wood smoke particles.

Conclusions: Exposure to wood smoke had no effect on markers of oxidative stress, DNA damage, cell adhesion, cytokines or MVF in atopic subjects.

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Related in: MedlinePlus

Microvascular function (MVF) score 6 h after termination of each of the different exposures (n = 20). Each symbol corresponds to a person, whereas the bars represent the mean of each exposure. The mean ± SEM were 1.61 ± 0.09, 1.51 ± 0.09, 1.55 ± 0.08 in the subjects exposed to clean air, low and high content of wood smoke particles, respectively.
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Figure 2: Microvascular function (MVF) score 6 h after termination of each of the different exposures (n = 20). Each symbol corresponds to a person, whereas the bars represent the mean of each exposure. The mean ± SEM were 1.61 ± 0.09, 1.51 ± 0.09, 1.55 ± 0.08 in the subjects exposed to clean air, low and high content of wood smoke particles, respectively.

Mentions: The results from the assessment of MVF are outlined in Figure 2. Two pulse wave tracings of MVF were not recorded due to instrument failure. We found no significant associations between the exposure and MVF score (p = 0.78). The average relative change (95% confidence interval) in MVF from the value measured after clean air exposure was 2% (-9% to 13%), 5% (-5% to 16%) and 3% (-5% to 10%) after low and high level of wood smoke exposure and for the mean value after the two exposure levels, respectively. The overall average MVF score, mean ± SEM, was relatively low (1.56 ± 0.04) in this group of atopic subjects compared to our earlier observations in healthy subjects, elderly, and the threshold for abnormal MVF score. In our previous investigations we have assessed the MVF in the morning, whereas the same endpoint was obtained in the late afternoon in this wood smoke exposure study.


Controlled human wood smoke exposure: oxidative stress, inflammation and microvascular function.

Forchhammer L, Møller P, Riddervold IS, Bønløkke J, Massling A, Sigsgaard T, Loft S - Part Fibre Toxicol (2012)

Microvascular function (MVF) score 6 h after termination of each of the different exposures (n = 20). Each symbol corresponds to a person, whereas the bars represent the mean of each exposure. The mean ± SEM were 1.61 ± 0.09, 1.51 ± 0.09, 1.55 ± 0.08 in the subjects exposed to clean air, low and high content of wood smoke particles, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369202&req=5

Figure 2: Microvascular function (MVF) score 6 h after termination of each of the different exposures (n = 20). Each symbol corresponds to a person, whereas the bars represent the mean of each exposure. The mean ± SEM were 1.61 ± 0.09, 1.51 ± 0.09, 1.55 ± 0.08 in the subjects exposed to clean air, low and high content of wood smoke particles, respectively.
Mentions: The results from the assessment of MVF are outlined in Figure 2. Two pulse wave tracings of MVF were not recorded due to instrument failure. We found no significant associations between the exposure and MVF score (p = 0.78). The average relative change (95% confidence interval) in MVF from the value measured after clean air exposure was 2% (-9% to 13%), 5% (-5% to 16%) and 3% (-5% to 10%) after low and high level of wood smoke exposure and for the mean value after the two exposure levels, respectively. The overall average MVF score, mean ± SEM, was relatively low (1.56 ± 0.04) in this group of atopic subjects compared to our earlier observations in healthy subjects, elderly, and the threshold for abnormal MVF score. In our previous investigations we have assessed the MVF in the morning, whereas the same endpoint was obtained in the late afternoon in this wood smoke exposure study.

Bottom Line: The MVF score was unaltered after inhalation of clean air (1.58 ± 0.07; mean ± SEM), low (1.51 ± 0.07) or high (1.61 ± 0.09) concentrations of wood smoke particles in atopic subjects, whereas unexposed non-atopic subjects had higher score (1.91 ± 0.09).The level of oxidatively damaged DNA, mRNA of ITGAL, CCL2, TNF, IL6, IL8, HMOX1, and OGG1 and surface marker molecules ICAM1, ITGAL and L-selectin in peripheral blood mononuclear cells were not affected by inhalation of wood smoke particles.Exposure to wood smoke had no effect on markers of oxidative stress, DNA damage, cell adhesion, cytokines or MVF in atopic subjects.

View Article: PubMed Central - HTML - PubMed

Affiliation: Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark.

ABSTRACT

Background: Exposure to wood smoke is associated with respiratory symptoms, whereas knowledge on systemic effects is limited. We investigated effects on systemic inflammation, oxidative stress and microvascular function (MVF) after controlled wood smoke exposure.

Methods: In a randomised, double-blinded, cross-over study 20 non-smoking atopic subjects were exposed at rest to 14, 220, or 354 μg/m3 of particles from a well-burning modern wood stove for 3 h in a climate controlled chamber with 2 week intervals. We investigated the level of oxidatively damaged DNA, inflammatory markers and adhesion molecules before and 0, 6 and 20 h after exposure. Six h after exposure we measured MVF non-invasively by digital peripheral artery tonometry following arm ischemia.

Results: The MVF score was unaltered after inhalation of clean air (1.58 ± 0.07; mean ± SEM), low (1.51 ± 0.07) or high (1.61 ± 0.09) concentrations of wood smoke particles in atopic subjects, whereas unexposed non-atopic subjects had higher score (1.91 ± 0.09). The level of oxidatively damaged DNA, mRNA of ITGAL, CCL2, TNF, IL6, IL8, HMOX1, and OGG1 and surface marker molecules ICAM1, ITGAL and L-selectin in peripheral blood mononuclear cells were not affected by inhalation of wood smoke particles.

Conclusions: Exposure to wood smoke had no effect on markers of oxidative stress, DNA damage, cell adhesion, cytokines or MVF in atopic subjects.

Show MeSH
Related in: MedlinePlus