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Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node.

Heuveling DA, Visser GW, de Groot M, de Boer JF, Baclayon M, Roos WH, Wuite GJ, Leemans CR, de Bree R, van Dongen GA - Eur. J. Nucl. Med. Mol. Imaging (2012)

Bottom Line: Conjugation of IRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted in a stable and pure product without affecting the particle size of the nanocolloidal albumin.No decrease in the fluorescence signal from SN was observed 24 h after injection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease or complete disappearance of the fluorescence signal was seen 24 h after injection of ICG/HSA.Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal kinetic features for SN detection.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center, Amsterdam, The Netherlands.

ABSTRACT

Purpose: At present, the only approved fluorescent tracer for clinical near-infrared fluorescence-guided sentinel node (SN) detection is indocyanine green (ICG), but the use of this tracer is limited due to its poor retention in the SN resulting in the detection of higher tier nodes. We describe the development and characterization of a next-generation fluorescent tracer, nanocolloidal albumin-IRDye 800CW that has optimal properties for clinical SN detection.

Methods: The fluorescent dye IRDye 800CW was covalently coupled to colloidal human serum albumin (HSA) particles present in the labelling kit Nanocoll in a manner compliant with current Good Manufacturing Practice. Characterization of nanocolloidal albumin-IRDye 800CW included determination of conjugation efficiency, purity, stability and particle size. Quantum yield was determined in serum and compared to that of ICG. For in vivo evaluation a lymphogenic metastatic tumour model in rabbits was used. Fluorescence imaging was performed directly after peritumoral injection of nanocolloidal albumin-IRDye 800CW or the reference ICG/HSA (i.e. ICG mixed with HSA), and was repeated after 24 h, after which fluorescent lymph nodes were excised.

Results: Conjugation of IRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted in a stable and pure product without affecting the particle size of the nanocolloidal albumin. The quantum yield of nanocolloidal albumin-IRDye 800CW was similar to that of ICG. In vivo evaluation revealed noninvasive detection of the SN within 5 min of injection of either nanocolloidal albumin-IRDye 800CW or ICG/HSA. No decrease in the fluorescence signal from SN was observed 24 h after injection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease or complete disappearance of the fluorescence signal was seen 24 h after injection of ICG/HSA. Fluorescence-guided SN biopsy was very easy.

Conclusion: Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal kinetic features for SN detection.

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Related in: MedlinePlus

In vivo evaluation of nanocolloidal albumin-IRDye 800CW. a Rabbit VX2 auricular carcinoma model. The red box represents the field of view of images b–e. b, c NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (b) and 24 h (c) after peritumoral injection of nanocolloidal albumin-IRDye 800CW (exposure time 500 ms). d, e NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (d) and 24 h (e) after peritumoral injection of ICG/HSA (exposure time 1000 ms) (1 parotid lymph node, 2 caudal mandibular lymph nodes, a angle of mandible, T tumour)
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Fig2: In vivo evaluation of nanocolloidal albumin-IRDye 800CW. a Rabbit VX2 auricular carcinoma model. The red box represents the field of view of images b–e. b, c NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (b) and 24 h (c) after peritumoral injection of nanocolloidal albumin-IRDye 800CW (exposure time 500 ms). d, e NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (d) and 24 h (e) after peritumoral injection of ICG/HSA (exposure time 1000 ms) (1 parotid lymph node, 2 caudal mandibular lymph nodes, a angle of mandible, T tumour)

Mentions: For in vivo evaluation, New Zealand white rabbits bearing auricular VX2 carcinomas were used. This animal model (Fig. 2a) is attractive for evaluating SN detection procedures in HNSCC, since after inoculation of tumour cells into the external ear the developing tumours tend to metastasize lymphatically. The first lymph node that is metastatically involved in this model is the parotid lymph node, the “SN”; caudal mandibular lymph nodes are considered to be higher tier lymph nodes in this model. The consistent lymphogenic metastatic spread of the tumour and the accessibility of the lymph nodes for surgery make this a very suitable model for evaluation of SN detection [18, 19]Fig. 2


Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node.

Heuveling DA, Visser GW, de Groot M, de Boer JF, Baclayon M, Roos WH, Wuite GJ, Leemans CR, de Bree R, van Dongen GA - Eur. J. Nucl. Med. Mol. Imaging (2012)

In vivo evaluation of nanocolloidal albumin-IRDye 800CW. a Rabbit VX2 auricular carcinoma model. The red box represents the field of view of images b–e. b, c NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (b) and 24 h (c) after peritumoral injection of nanocolloidal albumin-IRDye 800CW (exposure time 500 ms). d, e NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (d) and 24 h (e) after peritumoral injection of ICG/HSA (exposure time 1000 ms) (1 parotid lymph node, 2 caudal mandibular lymph nodes, a angle of mandible, T tumour)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369133&req=5

Fig2: In vivo evaluation of nanocolloidal albumin-IRDye 800CW. a Rabbit VX2 auricular carcinoma model. The red box represents the field of view of images b–e. b, c NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (b) and 24 h (c) after peritumoral injection of nanocolloidal albumin-IRDye 800CW (exposure time 500 ms). d, e NIR fluorescence image of fluorescence-labelled lymph nodes obtained 5 min (d) and 24 h (e) after peritumoral injection of ICG/HSA (exposure time 1000 ms) (1 parotid lymph node, 2 caudal mandibular lymph nodes, a angle of mandible, T tumour)
Mentions: For in vivo evaluation, New Zealand white rabbits bearing auricular VX2 carcinomas were used. This animal model (Fig. 2a) is attractive for evaluating SN detection procedures in HNSCC, since after inoculation of tumour cells into the external ear the developing tumours tend to metastasize lymphatically. The first lymph node that is metastatically involved in this model is the parotid lymph node, the “SN”; caudal mandibular lymph nodes are considered to be higher tier lymph nodes in this model. The consistent lymphogenic metastatic spread of the tumour and the accessibility of the lymph nodes for surgery make this a very suitable model for evaluation of SN detection [18, 19]Fig. 2

Bottom Line: Conjugation of IRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted in a stable and pure product without affecting the particle size of the nanocolloidal albumin.No decrease in the fluorescence signal from SN was observed 24 h after injection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease or complete disappearance of the fluorescence signal was seen 24 h after injection of ICG/HSA.Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal kinetic features for SN detection.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center, Amsterdam, The Netherlands.

ABSTRACT

Purpose: At present, the only approved fluorescent tracer for clinical near-infrared fluorescence-guided sentinel node (SN) detection is indocyanine green (ICG), but the use of this tracer is limited due to its poor retention in the SN resulting in the detection of higher tier nodes. We describe the development and characterization of a next-generation fluorescent tracer, nanocolloidal albumin-IRDye 800CW that has optimal properties for clinical SN detection.

Methods: The fluorescent dye IRDye 800CW was covalently coupled to colloidal human serum albumin (HSA) particles present in the labelling kit Nanocoll in a manner compliant with current Good Manufacturing Practice. Characterization of nanocolloidal albumin-IRDye 800CW included determination of conjugation efficiency, purity, stability and particle size. Quantum yield was determined in serum and compared to that of ICG. For in vivo evaluation a lymphogenic metastatic tumour model in rabbits was used. Fluorescence imaging was performed directly after peritumoral injection of nanocolloidal albumin-IRDye 800CW or the reference ICG/HSA (i.e. ICG mixed with HSA), and was repeated after 24 h, after which fluorescent lymph nodes were excised.

Results: Conjugation of IRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted in a stable and pure product without affecting the particle size of the nanocolloidal albumin. The quantum yield of nanocolloidal albumin-IRDye 800CW was similar to that of ICG. In vivo evaluation revealed noninvasive detection of the SN within 5 min of injection of either nanocolloidal albumin-IRDye 800CW or ICG/HSA. No decrease in the fluorescence signal from SN was observed 24 h after injection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease or complete disappearance of the fluorescence signal was seen 24 h after injection of ICG/HSA. Fluorescence-guided SN biopsy was very easy.

Conclusion: Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal kinetic features for SN detection.

Show MeSH
Related in: MedlinePlus