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Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development.

Xu P, Xu J, Li Z, Yang Z - PLoS ONE (2012)

Bottom Line: In both the brain and kidney, TRPC6 serves a variety of functions.Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex.Meanwhile, an increased expression was found in the hippocampus with the development.

View Article: PubMed Central - PubMed

Affiliation: College of Medicine, Nankai University, Tianjin, China.

ABSTRACT
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.

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Localization of TRPC6 in mouse hippocampus during the postnatal development.The immunohistochemical staining showed that TRPC6 was expressed in all regions of the hippocampus at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F) and P21 (G). The negative control image was shown in (A). Scale bar, 200 µm. In suit hybridization of P1 (H) and P5 (I) brain sections showed the expression of TRPC6 in hippocampal neurons. Scale bar, 100 µm. The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.
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pone-0038503-g003: Localization of TRPC6 in mouse hippocampus during the postnatal development.The immunohistochemical staining showed that TRPC6 was expressed in all regions of the hippocampus at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F) and P21 (G). The negative control image was shown in (A). Scale bar, 200 µm. In suit hybridization of P1 (H) and P5 (I) brain sections showed the expression of TRPC6 in hippocampal neurons. Scale bar, 100 µm. The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.

Mentions: It was found that the hippocampus developed and maturated gradually after birth. The volume of hippocampus increased slowly before P7 (Figure 3. B-E), and turned to be fast from P7, with a peak at P14 (Figure 3. F). After this fluctuation, the development rate became stabilize (Figure 3. G).


Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development.

Xu P, Xu J, Li Z, Yang Z - PLoS ONE (2012)

Localization of TRPC6 in mouse hippocampus during the postnatal development.The immunohistochemical staining showed that TRPC6 was expressed in all regions of the hippocampus at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F) and P21 (G). The negative control image was shown in (A). Scale bar, 200 µm. In suit hybridization of P1 (H) and P5 (I) brain sections showed the expression of TRPC6 in hippocampal neurons. Scale bar, 100 µm. The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368853&req=5

pone-0038503-g003: Localization of TRPC6 in mouse hippocampus during the postnatal development.The immunohistochemical staining showed that TRPC6 was expressed in all regions of the hippocampus at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F) and P21 (G). The negative control image was shown in (A). Scale bar, 200 µm. In suit hybridization of P1 (H) and P5 (I) brain sections showed the expression of TRPC6 in hippocampal neurons. Scale bar, 100 µm. The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.
Mentions: It was found that the hippocampus developed and maturated gradually after birth. The volume of hippocampus increased slowly before P7 (Figure 3. B-E), and turned to be fast from P7, with a peak at P14 (Figure 3. F). After this fluctuation, the development rate became stabilize (Figure 3. G).

Bottom Line: In both the brain and kidney, TRPC6 serves a variety of functions.Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex.Meanwhile, an increased expression was found in the hippocampus with the development.

View Article: PubMed Central - PubMed

Affiliation: College of Medicine, Nankai University, Tianjin, China.

ABSTRACT
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.

Show MeSH