Limits...
Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development.

Xu P, Xu J, Li Z, Yang Z - PLoS ONE (2012)

Bottom Line: In both the brain and kidney, TRPC6 serves a variety of functions.Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex.Meanwhile, an increased expression was found in the hippocampus with the development.

View Article: PubMed Central - PubMed

Affiliation: College of Medicine, Nankai University, Tianjin, China.

ABSTRACT
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.

Show MeSH
Immunoblot analysis of TRPC6 in mouse renal cortex during the development.The position of 106 kDa molecular size was the expression pattern of TRPC6 in renal cortex at the indicated ages (A). Quantitative determination of immunoblot showed significant changes in TRPC6 expression in mouse renal cortex during the development (B). The expression of TRPC6 was normalized to β-actin expression. Data were presented as means±S.D. *P<0.05, **P<0.01 vs. adjacent age group.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368853&req=5

pone-0038503-g002: Immunoblot analysis of TRPC6 in mouse renal cortex during the development.The position of 106 kDa molecular size was the expression pattern of TRPC6 in renal cortex at the indicated ages (A). Quantitative determination of immunoblot showed significant changes in TRPC6 expression in mouse renal cortex during the development (B). The expression of TRPC6 was normalized to β-actin expression. Data were presented as means±S.D. *P<0.05, **P<0.01 vs. adjacent age group.

Mentions: To identify developmental changes in the protein expression, the expression of TRPC6 was examined by Western blotting as β-actin was a consult. For these experiments, the cortex was separated from the kidney. As seen in Figure 2. A, single immunoreactive bands of 106 kDa were observed from cortex lysates. In addition, the quantitative immunoblot analysis showed that the expression of TRPC6 was decreased. During this period, the biggest change of TRPC6 level was between P7 and P14, and it was stable after P28 (Figure 2. B).


Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development.

Xu P, Xu J, Li Z, Yang Z - PLoS ONE (2012)

Immunoblot analysis of TRPC6 in mouse renal cortex during the development.The position of 106 kDa molecular size was the expression pattern of TRPC6 in renal cortex at the indicated ages (A). Quantitative determination of immunoblot showed significant changes in TRPC6 expression in mouse renal cortex during the development (B). The expression of TRPC6 was normalized to β-actin expression. Data were presented as means±S.D. *P<0.05, **P<0.01 vs. adjacent age group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368853&req=5

pone-0038503-g002: Immunoblot analysis of TRPC6 in mouse renal cortex during the development.The position of 106 kDa molecular size was the expression pattern of TRPC6 in renal cortex at the indicated ages (A). Quantitative determination of immunoblot showed significant changes in TRPC6 expression in mouse renal cortex during the development (B). The expression of TRPC6 was normalized to β-actin expression. Data were presented as means±S.D. *P<0.05, **P<0.01 vs. adjacent age group.
Mentions: To identify developmental changes in the protein expression, the expression of TRPC6 was examined by Western blotting as β-actin was a consult. For these experiments, the cortex was separated from the kidney. As seen in Figure 2. A, single immunoreactive bands of 106 kDa were observed from cortex lysates. In addition, the quantitative immunoblot analysis showed that the expression of TRPC6 was decreased. During this period, the biggest change of TRPC6 level was between P7 and P14, and it was stable after P28 (Figure 2. B).

Bottom Line: In both the brain and kidney, TRPC6 serves a variety of functions.Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex.Meanwhile, an increased expression was found in the hippocampus with the development.

View Article: PubMed Central - PubMed

Affiliation: College of Medicine, Nankai University, Tianjin, China.

ABSTRACT
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.

Show MeSH