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Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development.

Xu P, Xu J, Li Z, Yang Z - PLoS ONE (2012)

Bottom Line: In both the brain and kidney, TRPC6 serves a variety of functions.Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex.Meanwhile, an increased expression was found in the hippocampus with the development.

View Article: PubMed Central - PubMed

Affiliation: College of Medicine, Nankai University, Tianjin, China.

ABSTRACT
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.

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Localization of TRPC6 in mouse renal cortex during postnatal development.Immunohistochemical staining of TRPC6 showed the expression of TRPC6 in the mouse renal cortex at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F), P21 (G), P28 (H), and P49 (I). The negative control image was from the renal cortex, in which the primary antibody was a species-appropriate IgG (A). The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). The sections from P1 to P5 showed that TRPC6 mostly expressed in comma-shaped body, S-shaped body and renal corpuscles of stage III (B-D). After P7 the expression was found in renal corpuscles and it was weakly positive (E-F). During the development, tubules were all observed. Scale bar, 100 µm. Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.
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pone-0038503-g001: Localization of TRPC6 in mouse renal cortex during postnatal development.Immunohistochemical staining of TRPC6 showed the expression of TRPC6 in the mouse renal cortex at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F), P21 (G), P28 (H), and P49 (I). The negative control image was from the renal cortex, in which the primary antibody was a species-appropriate IgG (A). The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). The sections from P1 to P5 showed that TRPC6 mostly expressed in comma-shaped body, S-shaped body and renal corpuscles of stage III (B-D). After P7 the expression was found in renal corpuscles and it was weakly positive (E-F). During the development, tubules were all observed. Scale bar, 100 µm. Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.

Mentions: To identify the morphological and developmental changes in the protein expression, the specific antibody was used to mark the TRPC6 detection during the postnatal development in mice. It was known that there were four developmental stages of renal corpuscles: comma-shaped body, S-shaped body, renal corpuscles of stage III and renal corpuscles of stage IV [25]. In the renal cortex of immature mouse postnatal day 1–5 (P1–P5), it was observed that S-shaped body appeared mostly in every stage of renal corpuscles and comma-shaped body (Figure 1. B-D). At P7, there were renal corpuscles of stage IV only (Figure 1. E). Moreover,the number of corpuscles was proliferated prominently from P1 to P7. And the volume of cortex was increased after P7.


Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development.

Xu P, Xu J, Li Z, Yang Z - PLoS ONE (2012)

Localization of TRPC6 in mouse renal cortex during postnatal development.Immunohistochemical staining of TRPC6 showed the expression of TRPC6 in the mouse renal cortex at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F), P21 (G), P28 (H), and P49 (I). The negative control image was from the renal cortex, in which the primary antibody was a species-appropriate IgG (A). The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). The sections from P1 to P5 showed that TRPC6 mostly expressed in comma-shaped body, S-shaped body and renal corpuscles of stage III (B-D). After P7 the expression was found in renal corpuscles and it was weakly positive (E-F). During the development, tubules were all observed. Scale bar, 100 µm. Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368853&req=5

pone-0038503-g001: Localization of TRPC6 in mouse renal cortex during postnatal development.Immunohistochemical staining of TRPC6 showed the expression of TRPC6 in the mouse renal cortex at P1 (B), P3 (C), P5 (D), P7 (E), P14 (F), P21 (G), P28 (H), and P49 (I). The negative control image was from the renal cortex, in which the primary antibody was a species-appropriate IgG (A). The corresponding linear diagram of relative fluorescent intensity in glomeruli was shown in (J). The sections from P1 to P5 showed that TRPC6 mostly expressed in comma-shaped body, S-shaped body and renal corpuscles of stage III (B-D). After P7 the expression was found in renal corpuscles and it was weakly positive (E-F). During the development, tubules were all observed. Scale bar, 100 µm. Data were presented as means±S.D. n = 6/group. *P<0.05, **P<0.01 vs. adjacent age group.
Mentions: To identify the morphological and developmental changes in the protein expression, the specific antibody was used to mark the TRPC6 detection during the postnatal development in mice. It was known that there were four developmental stages of renal corpuscles: comma-shaped body, S-shaped body, renal corpuscles of stage III and renal corpuscles of stage IV [25]. In the renal cortex of immature mouse postnatal day 1–5 (P1–P5), it was observed that S-shaped body appeared mostly in every stage of renal corpuscles and comma-shaped body (Figure 1. B-D). At P7, there were renal corpuscles of stage IV only (Figure 1. E). Moreover,the number of corpuscles was proliferated prominently from P1 to P7. And the volume of cortex was increased after P7.

Bottom Line: In both the brain and kidney, TRPC6 serves a variety of functions.Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex.Meanwhile, an increased expression was found in the hippocampus with the development.

View Article: PubMed Central - PubMed

Affiliation: College of Medicine, Nankai University, Tianjin, China.

ABSTRACT
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.

Show MeSH