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Statistical optimization of process variables for antibiotic activity of Xenorhabdus bovienii.

Fang XL, Han LR, Cao XQ, Zhu MX, Zhang X, Wang YH - PLoS ONE (2012)

Bottom Line: A 2(5-1) factorial central composite design was chosen to determine the combined effects of the five variables, and to design a minimum number of experiments.The experimental and predicted antibiotic activity of X. bovienii YL002 was in close agreement.After optimization, the antibiotic activity was improved by 23.02% as compared with that of unoptimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling, Shaanxi, People's Republic of China.

ABSTRACT
The production of secondary metabolites with antibiotic properties is a common characteristic to entomopathogenic bacteria Xenorhabdus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities of medicinal and agricultural interests. Culture variables are critical to the production of secondary metabolites of microorganisms. Manipulating culture process variables can promote secondary metabolite biosynthesis and thus facilitate the discovery of novel natural products. This work was conducted to evaluate the effects of five process variables (initial pH, medium volume, rotary speed, temperature, and inoculation volume) on the antibiotic production of Xenorhabdus bovienii YL002 using response surface methodology. A 2(5-1) factorial central composite design was chosen to determine the combined effects of the five variables, and to design a minimum number of experiments. The experimental and predicted antibiotic activity of X. bovienii YL002 was in close agreement. Statistical analysis of the results showed that initial pH, medium volume, rotary speed and temperature had a significant effect (P<0.05) on the antibiotic production of X. bovienii YL002 at their individual level; medium volume and rotary speed showed a significant effect at a combined level and was most significant at an individual level. The maximum antibiotic activity (287.5 U/mL) was achieved at the initial pH of 8.24, medium volume of 54 mL in 250 mL flask, rotary speed of 208 rpm, temperature of 32.0°C and inoculation volume of 13.8%. After optimization, the antibiotic activity was improved by 23.02% as compared with that of unoptimized conditions.

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Differential metabolite profiles.Xenorhabdus bovienii YL002 was cultured under optimized condition and unoptimized condition, and organic extracts were assessed by high-pressure liquid chromatography (280 nm).
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pone-0038421-g005: Differential metabolite profiles.Xenorhabdus bovienii YL002 was cultured under optimized condition and unoptimized condition, and organic extracts were assessed by high-pressure liquid chromatography (280 nm).

Mentions: Metabolite profile of organic extracts from X. bovienii YL002 under both optimized condition and unoptimized condition by HPLC revealed that the production of metabolites was regulated by culture conditions. The metabolite profile at 32 min under optimized condition was significantly up regulated compared with that under unoptimized condition while it was slightly down regulated at 40 min (Fig. 5).


Statistical optimization of process variables for antibiotic activity of Xenorhabdus bovienii.

Fang XL, Han LR, Cao XQ, Zhu MX, Zhang X, Wang YH - PLoS ONE (2012)

Differential metabolite profiles.Xenorhabdus bovienii YL002 was cultured under optimized condition and unoptimized condition, and organic extracts were assessed by high-pressure liquid chromatography (280 nm).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368850&req=5

pone-0038421-g005: Differential metabolite profiles.Xenorhabdus bovienii YL002 was cultured under optimized condition and unoptimized condition, and organic extracts were assessed by high-pressure liquid chromatography (280 nm).
Mentions: Metabolite profile of organic extracts from X. bovienii YL002 under both optimized condition and unoptimized condition by HPLC revealed that the production of metabolites was regulated by culture conditions. The metabolite profile at 32 min under optimized condition was significantly up regulated compared with that under unoptimized condition while it was slightly down regulated at 40 min (Fig. 5).

Bottom Line: A 2(5-1) factorial central composite design was chosen to determine the combined effects of the five variables, and to design a minimum number of experiments.The experimental and predicted antibiotic activity of X. bovienii YL002 was in close agreement.After optimization, the antibiotic activity was improved by 23.02% as compared with that of unoptimized conditions.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling, Shaanxi, People's Republic of China.

ABSTRACT
The production of secondary metabolites with antibiotic properties is a common characteristic to entomopathogenic bacteria Xenorhabdus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities of medicinal and agricultural interests. Culture variables are critical to the production of secondary metabolites of microorganisms. Manipulating culture process variables can promote secondary metabolite biosynthesis and thus facilitate the discovery of novel natural products. This work was conducted to evaluate the effects of five process variables (initial pH, medium volume, rotary speed, temperature, and inoculation volume) on the antibiotic production of Xenorhabdus bovienii YL002 using response surface methodology. A 2(5-1) factorial central composite design was chosen to determine the combined effects of the five variables, and to design a minimum number of experiments. The experimental and predicted antibiotic activity of X. bovienii YL002 was in close agreement. Statistical analysis of the results showed that initial pH, medium volume, rotary speed and temperature had a significant effect (P<0.05) on the antibiotic production of X. bovienii YL002 at their individual level; medium volume and rotary speed showed a significant effect at a combined level and was most significant at an individual level. The maximum antibiotic activity (287.5 U/mL) was achieved at the initial pH of 8.24, medium volume of 54 mL in 250 mL flask, rotary speed of 208 rpm, temperature of 32.0°C and inoculation volume of 13.8%. After optimization, the antibiotic activity was improved by 23.02% as compared with that of unoptimized conditions.

Show MeSH
Related in: MedlinePlus