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Cellular basis of tissue regeneration by omentum.

Shah S, Lowery E, Braun RK, Martin A, Huang N, Medina M, Sethupathi P, Seki Y, Takami M, Byrne K, Wigfield C, Love RB, Iwashima M - PLoS ONE (2012)

Bottom Line: To understand the mechanism of tissue repair support by the omentum in more detail, we analyzed the cell subsets derived from the omentum on immune and inflammatory responses.Our data demonstrate that the omentum contains at least two groups of cells that support tissue repair, immunomodulatory myeloid derived suppressor cells and omnipotent stem cells that are indistinguishable from mesenchymal stem cells.Based on these data, we propose that the omentum is a designated organ for tissue repair and healing in response to foreign invasion and tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Chicago, Illinois, United States of America.

ABSTRACT
The omentum is a sheet-like tissue attached to the greater curvature of the stomach and contains secondary lymphoid organs called milky spots. The omentum has been used for its healing potential for over 100 years by transposing the omental pedicle to injured organs (omental transposition), but the mechanism by which omentum helps the healing process of damaged tissues is not well understood. Omental transposition promotes expansion of pancreatic islets, hepatocytes, embryonic kidney, and neurons. Omental cells (OCs) can be activated by foreign bodies in vivo. Once activated, they become a rich source for growth factors and express pluripotent stem cell markers. Moreover, OCs become engrafted in injured tissues suggesting that they might function as stem cells.Omentum consists of a variety of phenotypically and functionally distinctive cells. To understand the mechanism of tissue repair support by the omentum in more detail, we analyzed the cell subsets derived from the omentum on immune and inflammatory responses. Our data demonstrate that the omentum contains at least two groups of cells that support tissue repair, immunomodulatory myeloid derived suppressor cells and omnipotent stem cells that are indistinguishable from mesenchymal stem cells. Based on these data, we propose that the omentum is a designated organ for tissue repair and healing in response to foreign invasion and tissue damage.

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Presence of omentum-derived cells in the bleomycin-injured mouse lung.Omentum cells from transgenic mice that express GFP in non-hematopoietic cells or PBS (Bleo only) were injected into mice that underwent bleomycin-induced lung injury. 1 week after cell injection, mice were analyzed for the presence of GFP+ cells (green cells). (×10).
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pone-0038368-g007: Presence of omentum-derived cells in the bleomycin-injured mouse lung.Omentum cells from transgenic mice that express GFP in non-hematopoietic cells or PBS (Bleo only) were injected into mice that underwent bleomycin-induced lung injury. 1 week after cell injection, mice were analyzed for the presence of GFP+ cells (green cells). (×10).

Mentions: Lastly, we tested if omentum cells were integrated into lung tissues when injected into bleomycin-injected animals. To test this, we used omentum cells from transgenic mice that express GFP in a tissue non-specific manner [45]. When we adoptively transferred GFP+ omentum cells into bleomycin-injected animals, we detect the presence of GFP+ cells in the lung of bleomycin-injected animals (Fig. 7). Thus, a subset of the omentum cells, either as hematopoietic cells or lung epithelium cells, can migrate into the lung tissue.


Cellular basis of tissue regeneration by omentum.

Shah S, Lowery E, Braun RK, Martin A, Huang N, Medina M, Sethupathi P, Seki Y, Takami M, Byrne K, Wigfield C, Love RB, Iwashima M - PLoS ONE (2012)

Presence of omentum-derived cells in the bleomycin-injured mouse lung.Omentum cells from transgenic mice that express GFP in non-hematopoietic cells or PBS (Bleo only) were injected into mice that underwent bleomycin-induced lung injury. 1 week after cell injection, mice were analyzed for the presence of GFP+ cells (green cells). (×10).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368844&req=5

pone-0038368-g007: Presence of omentum-derived cells in the bleomycin-injured mouse lung.Omentum cells from transgenic mice that express GFP in non-hematopoietic cells or PBS (Bleo only) were injected into mice that underwent bleomycin-induced lung injury. 1 week after cell injection, mice were analyzed for the presence of GFP+ cells (green cells). (×10).
Mentions: Lastly, we tested if omentum cells were integrated into lung tissues when injected into bleomycin-injected animals. To test this, we used omentum cells from transgenic mice that express GFP in a tissue non-specific manner [45]. When we adoptively transferred GFP+ omentum cells into bleomycin-injected animals, we detect the presence of GFP+ cells in the lung of bleomycin-injected animals (Fig. 7). Thus, a subset of the omentum cells, either as hematopoietic cells or lung epithelium cells, can migrate into the lung tissue.

Bottom Line: To understand the mechanism of tissue repair support by the omentum in more detail, we analyzed the cell subsets derived from the omentum on immune and inflammatory responses.Our data demonstrate that the omentum contains at least two groups of cells that support tissue repair, immunomodulatory myeloid derived suppressor cells and omnipotent stem cells that are indistinguishable from mesenchymal stem cells.Based on these data, we propose that the omentum is a designated organ for tissue repair and healing in response to foreign invasion and tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Chicago, Illinois, United States of America.

ABSTRACT
The omentum is a sheet-like tissue attached to the greater curvature of the stomach and contains secondary lymphoid organs called milky spots. The omentum has been used for its healing potential for over 100 years by transposing the omental pedicle to injured organs (omental transposition), but the mechanism by which omentum helps the healing process of damaged tissues is not well understood. Omental transposition promotes expansion of pancreatic islets, hepatocytes, embryonic kidney, and neurons. Omental cells (OCs) can be activated by foreign bodies in vivo. Once activated, they become a rich source for growth factors and express pluripotent stem cell markers. Moreover, OCs become engrafted in injured tissues suggesting that they might function as stem cells.Omentum consists of a variety of phenotypically and functionally distinctive cells. To understand the mechanism of tissue repair support by the omentum in more detail, we analyzed the cell subsets derived from the omentum on immune and inflammatory responses. Our data demonstrate that the omentum contains at least two groups of cells that support tissue repair, immunomodulatory myeloid derived suppressor cells and omnipotent stem cells that are indistinguishable from mesenchymal stem cells. Based on these data, we propose that the omentum is a designated organ for tissue repair and healing in response to foreign invasion and tissue damage.

Show MeSH
Related in: MedlinePlus