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Distribution patterns of influenza virus receptors and viral attachment patterns in the respiratory and intestinal tracts of seven avian species.

Costa T, Chaves AJ, Valle R, Darji A, van Riel D, Kuiken T, Majó N, Ramis A - Vet. Res. (2012)

Bottom Line: Both α-2,3 and α-2,6 receptors were present in the respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, and golden pheasant.In ostriches, the expression of the receptor was basically restricted to α-2,3 in both the respiratory and intestinal tracts and in mallards the α-2,6 receptors were absent from the intestinal tract.The differential expression and distribution of α-2,3 and α-2,6 receptors among various avian species might reflect a potentially decisive factor in the emergence of new viral strains.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. antonio.ramis@uab.cat.

ABSTRACT
This study assessed the presence of sialic acid α-2,3 and α-2,6 linked glycan receptors in seven avian species. The respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, golden pheasant, ostrich, and mallard were tested by means of lectin histochemistry, using the lectins Maackia amurensis agglutinin II and Sambucus nigra agglutinin, which show affinity for α-2,3 and α-2,6 receptors, respectively. Additionally, the pattern of virus attachment (PVA) was evaluated with virus histochemistry, using an avian-origin H4N5 virus and a human-origin seasonal H1N1 virus. There was a great variation of receptor distribution among the tissues and avian species studied. Both α-2,3 and α-2,6 receptors were present in the respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, and golden pheasant. In ostriches, the expression of the receptor was basically restricted to α-2,3 in both the respiratory and intestinal tracts and in mallards the α-2,6 receptors were absent from the intestinal tract. The results obtained with the lectin histochemistry were, in general, in agreement with the PVA. The differential expression and distribution of α-2,3 and α-2,6 receptors among various avian species might reflect a potentially decisive factor in the emergence of new viral strains.

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Related in: MedlinePlus

Influenza receptor distribution and pattern of viral attachment in the trachea. Composite bright field microscope images comparing the distribution of α-2,3 and α-2,6 receptors, demonstrated by means of MAAII and SNA lectin histochemistry, with the pattern of viral attachment of the avian influenza A/Mallard/Netherlands/13/08 (H4N5) virus and the human influenza A/Netherlands/35/05 (H1N1) virus, demonstrated by means of virus histochemistry, in the trachea of chicken (A1-A4), common quail (B1-B4), red-legged partridge (C1-C4), turkey (D1-D4), golden pheasant (E1-E4), ostrich (F1-F4), and mallard (G1-G4).
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Figure 2: Influenza receptor distribution and pattern of viral attachment in the trachea. Composite bright field microscope images comparing the distribution of α-2,3 and α-2,6 receptors, demonstrated by means of MAAII and SNA lectin histochemistry, with the pattern of viral attachment of the avian influenza A/Mallard/Netherlands/13/08 (H4N5) virus and the human influenza A/Netherlands/35/05 (H1N1) virus, demonstrated by means of virus histochemistry, in the trachea of chicken (A1-A4), common quail (B1-B4), red-legged partridge (C1-C4), turkey (D1-D4), golden pheasant (E1-E4), ostrich (F1-F4), and mallard (G1-G4).

Mentions: There was a marked variation on the distribution and expression of influenza receptors among the tissues and avian species studied. The distribution of influenza receptors in the respiratory tract is given in Table 1 and the results from nasal cavity and trachea are illustrated in Figures 1 and 2, respectively.


Distribution patterns of influenza virus receptors and viral attachment patterns in the respiratory and intestinal tracts of seven avian species.

Costa T, Chaves AJ, Valle R, Darji A, van Riel D, Kuiken T, Majó N, Ramis A - Vet. Res. (2012)

Influenza receptor distribution and pattern of viral attachment in the trachea. Composite bright field microscope images comparing the distribution of α-2,3 and α-2,6 receptors, demonstrated by means of MAAII and SNA lectin histochemistry, with the pattern of viral attachment of the avian influenza A/Mallard/Netherlands/13/08 (H4N5) virus and the human influenza A/Netherlands/35/05 (H1N1) virus, demonstrated by means of virus histochemistry, in the trachea of chicken (A1-A4), common quail (B1-B4), red-legged partridge (C1-C4), turkey (D1-D4), golden pheasant (E1-E4), ostrich (F1-F4), and mallard (G1-G4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368784&req=5

Figure 2: Influenza receptor distribution and pattern of viral attachment in the trachea. Composite bright field microscope images comparing the distribution of α-2,3 and α-2,6 receptors, demonstrated by means of MAAII and SNA lectin histochemistry, with the pattern of viral attachment of the avian influenza A/Mallard/Netherlands/13/08 (H4N5) virus and the human influenza A/Netherlands/35/05 (H1N1) virus, demonstrated by means of virus histochemistry, in the trachea of chicken (A1-A4), common quail (B1-B4), red-legged partridge (C1-C4), turkey (D1-D4), golden pheasant (E1-E4), ostrich (F1-F4), and mallard (G1-G4).
Mentions: There was a marked variation on the distribution and expression of influenza receptors among the tissues and avian species studied. The distribution of influenza receptors in the respiratory tract is given in Table 1 and the results from nasal cavity and trachea are illustrated in Figures 1 and 2, respectively.

Bottom Line: Both α-2,3 and α-2,6 receptors were present in the respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, and golden pheasant.In ostriches, the expression of the receptor was basically restricted to α-2,3 in both the respiratory and intestinal tracts and in mallards the α-2,6 receptors were absent from the intestinal tract.The differential expression and distribution of α-2,3 and α-2,6 receptors among various avian species might reflect a potentially decisive factor in the emergence of new viral strains.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. antonio.ramis@uab.cat.

ABSTRACT
This study assessed the presence of sialic acid α-2,3 and α-2,6 linked glycan receptors in seven avian species. The respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, golden pheasant, ostrich, and mallard were tested by means of lectin histochemistry, using the lectins Maackia amurensis agglutinin II and Sambucus nigra agglutinin, which show affinity for α-2,3 and α-2,6 receptors, respectively. Additionally, the pattern of virus attachment (PVA) was evaluated with virus histochemistry, using an avian-origin H4N5 virus and a human-origin seasonal H1N1 virus. There was a great variation of receptor distribution among the tissues and avian species studied. Both α-2,3 and α-2,6 receptors were present in the respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, turkey, and golden pheasant. In ostriches, the expression of the receptor was basically restricted to α-2,3 in both the respiratory and intestinal tracts and in mallards the α-2,6 receptors were absent from the intestinal tract. The results obtained with the lectin histochemistry were, in general, in agreement with the PVA. The differential expression and distribution of α-2,3 and α-2,6 receptors among various avian species might reflect a potentially decisive factor in the emergence of new viral strains.

Show MeSH
Related in: MedlinePlus