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Proteomics approaches to fibrotic disorders.

Gucek M - Fibrogenesis Tissue Repair (2012)

Bottom Line: This review provides an introduction to mass spectrometry based proteomics and discusses several proteomics approaches that are relevant in understanding the pathophysiology of fibrotic disorders and the approaches that are frequently used in biomarker discovery.

View Article: PubMed Central - HTML - PubMed

Affiliation: NHLBI Proteomics Core , National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA.

ABSTRACT
This review provides an introduction to mass spectrometry based proteomics and discusses several proteomics approaches that are relevant in understanding the pathophysiology of fibrotic disorders and the approaches that are frequently used in biomarker discovery.

No MeSH data available.


Related in: MedlinePlus

Mass spectrometry based proteomics relies on the separation of proteins using gels or chromatography, digestion with an enzyme and further analysis by mass spectrometry. Bioinformatics tools help in identification and quantification of proteins.
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Figure 1: Mass spectrometry based proteomics relies on the separation of proteins using gels or chromatography, digestion with an enzyme and further analysis by mass spectrometry. Bioinformatics tools help in identification and quantification of proteins.

Mentions: Mass spectrometry is an indispensible tool for relative protein quantitation which is usually applied to discovery of clinical biomarkers and mostly uses large-scale proteomics experiments. The first step in a typical large scale proteomics experiment is a separation technique on either the protein (e.g. 1D gel) or peptide level (e.g. SCX - strong cation exchange chromatography), followed by analysis of each of the individual fractions with liquid chromatography coupled to tandem mass spectrometry system (LC-MS/MS). The liquid chromatography is mostly reverse phase based with the flows in nanoliter range. Usually, 1D gel separation on protein level followed by an LC-MS/MS analysis gives a higher number of identified proteins because the first dimension separation is on the protein level as compared to SCX which separates peptides on the basis of charge. A summary of proteomics approaches using mass spectrometry is depicted in Figure 1.


Proteomics approaches to fibrotic disorders.

Gucek M - Fibrogenesis Tissue Repair (2012)

Mass spectrometry based proteomics relies on the separation of proteins using gels or chromatography, digestion with an enzyme and further analysis by mass spectrometry. Bioinformatics tools help in identification and quantification of proteins.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368766&req=5

Figure 1: Mass spectrometry based proteomics relies on the separation of proteins using gels or chromatography, digestion with an enzyme and further analysis by mass spectrometry. Bioinformatics tools help in identification and quantification of proteins.
Mentions: Mass spectrometry is an indispensible tool for relative protein quantitation which is usually applied to discovery of clinical biomarkers and mostly uses large-scale proteomics experiments. The first step in a typical large scale proteomics experiment is a separation technique on either the protein (e.g. 1D gel) or peptide level (e.g. SCX - strong cation exchange chromatography), followed by analysis of each of the individual fractions with liquid chromatography coupled to tandem mass spectrometry system (LC-MS/MS). The liquid chromatography is mostly reverse phase based with the flows in nanoliter range. Usually, 1D gel separation on protein level followed by an LC-MS/MS analysis gives a higher number of identified proteins because the first dimension separation is on the protein level as compared to SCX which separates peptides on the basis of charge. A summary of proteomics approaches using mass spectrometry is depicted in Figure 1.

Bottom Line: This review provides an introduction to mass spectrometry based proteomics and discusses several proteomics approaches that are relevant in understanding the pathophysiology of fibrotic disorders and the approaches that are frequently used in biomarker discovery.

View Article: PubMed Central - HTML - PubMed

Affiliation: NHLBI Proteomics Core , National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA.

ABSTRACT
This review provides an introduction to mass spectrometry based proteomics and discusses several proteomics approaches that are relevant in understanding the pathophysiology of fibrotic disorders and the approaches that are frequently used in biomarker discovery.

No MeSH data available.


Related in: MedlinePlus