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Vapor phase mediated cellular uptake of sub 5 nm nanoparticles.

Serdiuk T, Lysenko V, Skryshevsky VA, Géloën A - Nanoscale Res Lett (2012)

Bottom Line: Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open.Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase.However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of Lyon, Nanotechnology Institute of Lyon (INL), UMR-5270, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Lyon, Villeurbanne, F-69621, France. tetiana.serdiuk@gmail.com.

ABSTRACT
Nanoparticles became an important and wide-used tool for cell imaging because of their unique optical properties. Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open. Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase. Natural gradual size-tuning of NPs in dependence to the distance from the NP liquid source allows progressive shift of the fluorescence color of labeled cells in the blue region according to the increase of the distance from the NP suspension. This effect may be used for the soft vapor labeling of biological cells with the possibility of controlling the color of fluorescence. However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

No MeSH data available.


Related in: MedlinePlus

Dependence of the integrated luminosity per one cell. The cell cultures were exposed to NPs via air phase on the different horizontal distance between them and the cell culture containing the colloidal fluorescent NPs.
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Figure 5: Dependence of the integrated luminosity per one cell. The cell cultures were exposed to NPs via air phase on the different horizontal distance between them and the cell culture containing the colloidal fluorescent NPs.

Mentions: Another essential application of the present observation concerns the contamination of control samples. Figure 4 shows that a control cell sample (3 T3-L1 mouse fibroblasts) situated close to a well containing colloidal NP suspension can be easily contaminated through the vapor phase. Indeed, as one can see, the corresponding fluorescent pictures of both cell cultures, control without NPs and experimental with NPs, are characterized by almost the same fluorescent intensities, while a reference completely isolated from all the colloidal NP suspensions remains fully unlabeled. Dependence of the fluorescent luminosity per one cell in function of the horizontal distance between the cell culture containing the colloidal fluorescent SiC NPs and the cell cultures labeled via air is shown in Figure 5. Intensity of the fluorescent labeling coming from the surrounding air containing NPs is inversely dependent to the distance from the intentionally labeled cell culture wells. A natural gradual size-tuning of SiC NPs was observed in dependence of the distance from the NP source. This results in a progressive shift of fluorescence color of labeled cells to the red region with the distance decrease. That effect may be used for the soft vapor labeling of biological cells, allowing for the control of their color. However, to avoid any parasitical contamination of experimental samples via vapor phase, one has to spatially isolate the cell cultures containing colloidal NP suspension.


Vapor phase mediated cellular uptake of sub 5 nm nanoparticles.

Serdiuk T, Lysenko V, Skryshevsky VA, Géloën A - Nanoscale Res Lett (2012)

Dependence of the integrated luminosity per one cell. The cell cultures were exposed to NPs via air phase on the different horizontal distance between them and the cell culture containing the colloidal fluorescent NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368743&req=5

Figure 5: Dependence of the integrated luminosity per one cell. The cell cultures were exposed to NPs via air phase on the different horizontal distance between them and the cell culture containing the colloidal fluorescent NPs.
Mentions: Another essential application of the present observation concerns the contamination of control samples. Figure 4 shows that a control cell sample (3 T3-L1 mouse fibroblasts) situated close to a well containing colloidal NP suspension can be easily contaminated through the vapor phase. Indeed, as one can see, the corresponding fluorescent pictures of both cell cultures, control without NPs and experimental with NPs, are characterized by almost the same fluorescent intensities, while a reference completely isolated from all the colloidal NP suspensions remains fully unlabeled. Dependence of the fluorescent luminosity per one cell in function of the horizontal distance between the cell culture containing the colloidal fluorescent SiC NPs and the cell cultures labeled via air is shown in Figure 5. Intensity of the fluorescent labeling coming from the surrounding air containing NPs is inversely dependent to the distance from the intentionally labeled cell culture wells. A natural gradual size-tuning of SiC NPs was observed in dependence of the distance from the NP source. This results in a progressive shift of fluorescence color of labeled cells to the red region with the distance decrease. That effect may be used for the soft vapor labeling of biological cells, allowing for the control of their color. However, to avoid any parasitical contamination of experimental samples via vapor phase, one has to spatially isolate the cell cultures containing colloidal NP suspension.

Bottom Line: Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open.Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase.However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of Lyon, Nanotechnology Institute of Lyon (INL), UMR-5270, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Lyon, Villeurbanne, F-69621, France. tetiana.serdiuk@gmail.com.

ABSTRACT
Nanoparticles became an important and wide-used tool for cell imaging because of their unique optical properties. Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open. Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase. Natural gradual size-tuning of NPs in dependence to the distance from the NP liquid source allows progressive shift of the fluorescence color of labeled cells in the blue region according to the increase of the distance from the NP suspension. This effect may be used for the soft vapor labeling of biological cells with the possibility of controlling the color of fluorescence. However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

No MeSH data available.


Related in: MedlinePlus