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Vapor phase mediated cellular uptake of sub 5 nm nanoparticles.

Serdiuk T, Lysenko V, Skryshevsky VA, Géloën A - Nanoscale Res Lett (2012)

Bottom Line: Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open.Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase.However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of Lyon, Nanotechnology Institute of Lyon (INL), UMR-5270, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Lyon, Villeurbanne, F-69621, France. tetiana.serdiuk@gmail.com.

ABSTRACT
Nanoparticles became an important and wide-used tool for cell imaging because of their unique optical properties. Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open. Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase. Natural gradual size-tuning of NPs in dependence to the distance from the NP liquid source allows progressive shift of the fluorescence color of labeled cells in the blue region according to the increase of the distance from the NP suspension. This effect may be used for the soft vapor labeling of biological cells with the possibility of controlling the color of fluorescence. However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

No MeSH data available.


Related in: MedlinePlus

Variation of fluorescence color of the onion cells labeled by the NPs via vapor phase. Fluorescence images of the onion cells situated at different vertical distances from the colloidal suspension of NPs.
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Figure 3: Variation of fluorescence color of the onion cells labeled by the NPs via vapor phase. Fluorescence images of the onion cells situated at different vertical distances from the colloidal suspension of NPs.

Mentions: Figure 3 shows the variation of fluorescence color of the onion cells labeled by the NPs via vapor phase as function of the distance to the colloidal NP suspension. The cell fluorescence is continuously red-shifted when the distance between the onion cells and the NP suspension surface decreases. Taking into account that due to the quantum confinement effect, the smaller the NPs are, the shorter their fluorescence wavelength is, it can be concluded that, obviously, smaller nanoparticles are much easier to be transported in vapor phase. In particular, according to the low mean of Bohr's diameter (5.4 nm), even very small changes of NP diameter provoke strong shift of photoluminescence maximum position.


Vapor phase mediated cellular uptake of sub 5 nm nanoparticles.

Serdiuk T, Lysenko V, Skryshevsky VA, Géloën A - Nanoscale Res Lett (2012)

Variation of fluorescence color of the onion cells labeled by the NPs via vapor phase. Fluorescence images of the onion cells situated at different vertical distances from the colloidal suspension of NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368743&req=5

Figure 3: Variation of fluorescence color of the onion cells labeled by the NPs via vapor phase. Fluorescence images of the onion cells situated at different vertical distances from the colloidal suspension of NPs.
Mentions: Figure 3 shows the variation of fluorescence color of the onion cells labeled by the NPs via vapor phase as function of the distance to the colloidal NP suspension. The cell fluorescence is continuously red-shifted when the distance between the onion cells and the NP suspension surface decreases. Taking into account that due to the quantum confinement effect, the smaller the NPs are, the shorter their fluorescence wavelength is, it can be concluded that, obviously, smaller nanoparticles are much easier to be transported in vapor phase. In particular, according to the low mean of Bohr's diameter (5.4 nm), even very small changes of NP diameter provoke strong shift of photoluminescence maximum position.

Bottom Line: Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open.Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase.However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of Lyon, Nanotechnology Institute of Lyon (INL), UMR-5270, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Lyon, Villeurbanne, F-69621, France. tetiana.serdiuk@gmail.com.

ABSTRACT
Nanoparticles became an important and wide-used tool for cell imaging because of their unique optical properties. Although the potential of nanoparticles (NPs) in biology is promising, a number of questions concerning the safety of nanomaterials and the risk/benefit ratio of their usage are open. Here, we have shown that nanoparticles produced from silicon carbide (NPs) dispersed in colloidal suspensions are able to penetrate into surrounding air environment during the natural evaporation of the colloids and label biological cells via vapor phase. Natural gradual size-tuning of NPs in dependence to the distance from the NP liquid source allows progressive shift of the fluorescence color of labeled cells in the blue region according to the increase of the distance from the NP suspension. This effect may be used for the soft vapor labeling of biological cells with the possibility of controlling the color of fluorescence. However, scientists dealing with the colloidal NPs have to seriously consider such a NP's natural transfer in order to protect their own health as well as to avoid any contamination of the control samples.

No MeSH data available.


Related in: MedlinePlus