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Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection.

Karniychuk UU, Van Breedam W, Van Roy N, Rogel-Gaillard C, Nauwynck HJ - Vet. Res. (2012)

Bottom Line: Male DNA was detected in female fetal sera of all dams via PCR.Male DNA was also detected in the maternal circulation.Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium. uladzimir.karniychuk@UGent.be.

ABSTRACT
The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.

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Microchimeric cells found in female and male samples. Red-dUTP and Green-dUTP (Abbott Molecular, USA) were used as fluorophores for the Y and X probes, respectively. FISH analysis on female lungs and male liver using probes for the X (green signal) and Y (red signal) chromosomes. Cell nuclei were counterstained with DAPI (blue). Arrows indicate microchimeric cells. FISH images of male and female cells were recorded using a Zeiss Axioplan 2 fluorescence microscope, a high-sensitivity integrated CCD camera and dedicated software (ISIS, MetaSystems, Germany).
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Figure 2: Microchimeric cells found in female and male samples. Red-dUTP and Green-dUTP (Abbott Molecular, USA) were used as fluorophores for the Y and X probes, respectively. FISH analysis on female lungs and male liver using probes for the X (green signal) and Y (red signal) chromosomes. Cell nuclei were counterstained with DAPI (blue). Arrows indicate microchimeric cells. FISH images of male and female cells were recorded using a Zeiss Axioplan 2 fluorescence microscope, a high-sensitivity integrated CCD camera and dedicated software (ISIS, MetaSystems, Germany).

Mentions: Male (XY) cells were detected in 12 out of 30 tested female fetuses; 25 out of 30 male fetuses harbored female cells (p < 0.05, tested with the Chi-square test, Additional file 4). Female cells were found at higher numbers within organs of male fetuses versus the number of male cells within organs of female fetuses (p < 0.05, tested with the Mann-Whitney rank sum test). Representative images of microchimeric cells found in female and male samples are shown in Figure 2.


Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection.

Karniychuk UU, Van Breedam W, Van Roy N, Rogel-Gaillard C, Nauwynck HJ - Vet. Res. (2012)

Microchimeric cells found in female and male samples. Red-dUTP and Green-dUTP (Abbott Molecular, USA) were used as fluorophores for the Y and X probes, respectively. FISH analysis on female lungs and male liver using probes for the X (green signal) and Y (red signal) chromosomes. Cell nuclei were counterstained with DAPI (blue). Arrows indicate microchimeric cells. FISH images of male and female cells were recorded using a Zeiss Axioplan 2 fluorescence microscope, a high-sensitivity integrated CCD camera and dedicated software (ISIS, MetaSystems, Germany).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368719&req=5

Figure 2: Microchimeric cells found in female and male samples. Red-dUTP and Green-dUTP (Abbott Molecular, USA) were used as fluorophores for the Y and X probes, respectively. FISH analysis on female lungs and male liver using probes for the X (green signal) and Y (red signal) chromosomes. Cell nuclei were counterstained with DAPI (blue). Arrows indicate microchimeric cells. FISH images of male and female cells were recorded using a Zeiss Axioplan 2 fluorescence microscope, a high-sensitivity integrated CCD camera and dedicated software (ISIS, MetaSystems, Germany).
Mentions: Male (XY) cells were detected in 12 out of 30 tested female fetuses; 25 out of 30 male fetuses harbored female cells (p < 0.05, tested with the Chi-square test, Additional file 4). Female cells were found at higher numbers within organs of male fetuses versus the number of male cells within organs of female fetuses (p < 0.05, tested with the Mann-Whitney rank sum test). Representative images of microchimeric cells found in female and male samples are shown in Figure 2.

Bottom Line: Male DNA was detected in female fetal sera of all dams via PCR.Male DNA was also detected in the maternal circulation.Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium. uladzimir.karniychuk@UGent.be.

ABSTRACT
The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.

Show MeSH
Related in: MedlinePlus