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Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection.

Karniychuk UU, Van Breedam W, Van Roy N, Rogel-Gaillard C, Nauwynck HJ - Vet. Res. (2012)

Bottom Line: Male DNA was detected in female fetal sera of all dams via PCR.Male DNA was also detected in the maternal circulation.Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium. uladzimir.karniychuk@UGent.be.

ABSTRACT
The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.

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Diagrams schematically represent the porcine uteri of the nine dams included in the study. Fetuses were numbered starting with the fetus located next to the ovarian tip of the left uterine horn. L and R: left and right uterine horn, respectively. Each circle represents an individual fetus. F: female and M: male fetuses. "+" and "-" within the circles represent PRRSV-positive and -negative fetuses. Filled circles are female fetuses that were tested for the presence of male sex-determining region Y (SRY) in serum via PCR. Red and green circles are SRY-positive or -negative fetuses, respectively. Female fetuses with non-filled circles were not tested due to the lack of serum ▲: in these fetuses microchimeric cells were found. na: not available; due to mummification the determination of fetal gender and PRRSV infection status was not possible.
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Figure 1: Diagrams schematically represent the porcine uteri of the nine dams included in the study. Fetuses were numbered starting with the fetus located next to the ovarian tip of the left uterine horn. L and R: left and right uterine horn, respectively. Each circle represents an individual fetus. F: female and M: male fetuses. "+" and "-" within the circles represent PRRSV-positive and -negative fetuses. Filled circles are female fetuses that were tested for the presence of male sex-determining region Y (SRY) in serum via PCR. Red and green circles are SRY-positive or -negative fetuses, respectively. Female fetuses with non-filled circles were not tested due to the lack of serum ▲: in these fetuses microchimeric cells were found. na: not available; due to mummification the determination of fetal gender and PRRSV infection status was not possible.

Mentions: In total, 66 sera from female fetuses were tested in the SRY and ZFX PCRs. The results are summarized in Figure 1 and Additional file 2. SRY was detected in female fetal sera from both non-inoculated and PRRSV-inoculated dams. Non-inoculated and PRRSV-inoculated dams, had 20-43% and 20-100% of SRY-positive female fetuses, respectively. Male DNA was also detected in the maternal circulation of pregnant dams before and after infection (Table 2). All female fetal and maternal samples were amplifiable by the ZFX assay.


Demonstration of microchimerism in pregnant sows and effects of congenital PRRSV infection.

Karniychuk UU, Van Breedam W, Van Roy N, Rogel-Gaillard C, Nauwynck HJ - Vet. Res. (2012)

Diagrams schematically represent the porcine uteri of the nine dams included in the study. Fetuses were numbered starting with the fetus located next to the ovarian tip of the left uterine horn. L and R: left and right uterine horn, respectively. Each circle represents an individual fetus. F: female and M: male fetuses. "+" and "-" within the circles represent PRRSV-positive and -negative fetuses. Filled circles are female fetuses that were tested for the presence of male sex-determining region Y (SRY) in serum via PCR. Red and green circles are SRY-positive or -negative fetuses, respectively. Female fetuses with non-filled circles were not tested due to the lack of serum ▲: in these fetuses microchimeric cells were found. na: not available; due to mummification the determination of fetal gender and PRRSV infection status was not possible.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368719&req=5

Figure 1: Diagrams schematically represent the porcine uteri of the nine dams included in the study. Fetuses were numbered starting with the fetus located next to the ovarian tip of the left uterine horn. L and R: left and right uterine horn, respectively. Each circle represents an individual fetus. F: female and M: male fetuses. "+" and "-" within the circles represent PRRSV-positive and -negative fetuses. Filled circles are female fetuses that were tested for the presence of male sex-determining region Y (SRY) in serum via PCR. Red and green circles are SRY-positive or -negative fetuses, respectively. Female fetuses with non-filled circles were not tested due to the lack of serum ▲: in these fetuses microchimeric cells were found. na: not available; due to mummification the determination of fetal gender and PRRSV infection status was not possible.
Mentions: In total, 66 sera from female fetuses were tested in the SRY and ZFX PCRs. The results are summarized in Figure 1 and Additional file 2. SRY was detected in female fetal sera from both non-inoculated and PRRSV-inoculated dams. Non-inoculated and PRRSV-inoculated dams, had 20-43% and 20-100% of SRY-positive female fetuses, respectively. Male DNA was also detected in the maternal circulation of pregnant dams before and after infection (Table 2). All female fetal and maternal samples were amplifiable by the ZFX assay.

Bottom Line: Male DNA was detected in female fetal sera of all dams via PCR.Male DNA was also detected in the maternal circulation.Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium. uladzimir.karniychuk@UGent.be.

ABSTRACT
The presence of foreign cells within the tissue/circulation of an individual is described as microchimerism. The main purpose of the present investigation was to study if microchimerism occurs in healthy sows/fetuses and if porcine reproductive and respiratory syndrome virus (PRRSV) infection influences this phenomenon. Six dams were inoculated intranasally with PRRSV and three non-inoculated dams served as controls. Male DNA was detected in female fetal sera of all dams via PCR. Male DNA was also detected in the maternal circulation. Sex-typing FISH showed the presence of male cells in the female fetal organs and vice versa. PRRSV infection did not influence microchimerism, but might misuse maternal and sibling microchimeric cells to enter fetuses.

Show MeSH
Related in: MedlinePlus