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High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

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In-vivo imaging of human thigh skin. (a) The photograph shows the imaging location (white rectangle area) on the thigh of the volunteer. (b)-(e) MIP view IF-IBDV images of microcirculation network at different depths of human skin with depth of: (b) 120 μm-360 μm; (c) 360 μm-600 μm; (d) 600 μm-840 μm; (e) 840 μm-1.3 mm. The arrows indicate new blood vessels detected in each image and the circles indicate new branches detected in the image. (f) The CED MIP view IBDV image for the depth of 120 μm-360 μm. (g) The CED MIP view IBDV image for the depth of 360 μm-1300 μm . The scale bars in (a) represent 1 mm.
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g009: In-vivo imaging of human thigh skin. (a) The photograph shows the imaging location (white rectangle area) on the thigh of the volunteer. (b)-(e) MIP view IF-IBDV images of microcirculation network at different depths of human skin with depth of: (b) 120 μm-360 μm; (c) 360 μm-600 μm; (d) 600 μm-840 μm; (e) 840 μm-1.3 mm. The arrows indicate new blood vessels detected in each image and the circles indicate new branches detected in the image. (f) The CED MIP view IBDV image for the depth of 120 μm-360 μm. (g) The CED MIP view IBDV image for the depth of 360 μm-1300 μm . The scale bars in (a) represent 1 mm.

Mentions: To demonstrate the capability of the IF-IDBV method, measurements were taken from human skin. Imaging was performed on a healthy adult male volunteer. The volunteer sat on a chair and held the probe himself. The imaging region was on the thigh of the volunteer [Fig. 9(a)Fig. 9


High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

In-vivo imaging of human thigh skin. (a) The photograph shows the imaging location (white rectangle area) on the thigh of the volunteer. (b)-(e) MIP view IF-IBDV images of microcirculation network at different depths of human skin with depth of: (b) 120 μm-360 μm; (c) 360 μm-600 μm; (d) 600 μm-840 μm; (e) 840 μm-1.3 mm. The arrows indicate new blood vessels detected in each image and the circles indicate new branches detected in the image. (f) The CED MIP view IBDV image for the depth of 120 μm-360 μm. (g) The CED MIP view IBDV image for the depth of 360 μm-1300 μm . The scale bars in (a) represent 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368711&req=5

g009: In-vivo imaging of human thigh skin. (a) The photograph shows the imaging location (white rectangle area) on the thigh of the volunteer. (b)-(e) MIP view IF-IBDV images of microcirculation network at different depths of human skin with depth of: (b) 120 μm-360 μm; (c) 360 μm-600 μm; (d) 600 μm-840 μm; (e) 840 μm-1.3 mm. The arrows indicate new blood vessels detected in each image and the circles indicate new branches detected in the image. (f) The CED MIP view IBDV image for the depth of 120 μm-360 μm. (g) The CED MIP view IBDV image for the depth of 360 μm-1300 μm . The scale bars in (a) represent 1 mm.
Mentions: To demonstrate the capability of the IF-IDBV method, measurements were taken from human skin. Imaging was performed on a healthy adult male volunteer. The volunteer sat on a chair and held the probe himself. The imaging region was on the thigh of the volunteer [Fig. 9(a)Fig. 9

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

Show MeSH
Related in: MedlinePlus