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High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

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Comparison of LSI and IF-IBDV images of microvascular network in DSWC model, after laser irradiation of select vessels. (a) With green filtering of broadband light used to transilluminate the DSWC, the blood-vessel architecture is clearly visible. (b) With LSI, vessels containing flowing blood are displayed, with an absence of flow in the irradiated vessels. (c) With IF-IBDV method, a similar functional map of the vasculature is obtained, with enhanced visualization of smaller arteriolar, venules, and capillaries. The scale bars in (a) represent 1 mm.
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g008: Comparison of LSI and IF-IBDV images of microvascular network in DSWC model, after laser irradiation of select vessels. (a) With green filtering of broadband light used to transilluminate the DSWC, the blood-vessel architecture is clearly visible. (b) With LSI, vessels containing flowing blood are displayed, with an absence of flow in the irradiated vessels. (c) With IF-IBDV method, a similar functional map of the vasculature is obtained, with enhanced visualization of smaller arteriolar, venules, and capillaries. The scale bars in (a) represent 1 mm.

Mentions: Laser irradiation was then performed on the subdermal side of the DSWC. Blood vessels were irradiated with a sequence of three pulses from a frequency-doubled Nd:YAG laser (Dualis VP+, Fotona Laser, Ljublijana, Slovenia) with pulse width of 1 millisecond (ms), energy density of 5 J/cm2, repetition rate of 20 Hz, and beam sport size of 2 mm. To verify the OCT IF-IBDV results, we also took transillumination images with a green color filter [Fig. 8(a)Fig. 8


High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

Comparison of LSI and IF-IBDV images of microvascular network in DSWC model, after laser irradiation of select vessels. (a) With green filtering of broadband light used to transilluminate the DSWC, the blood-vessel architecture is clearly visible. (b) With LSI, vessels containing flowing blood are displayed, with an absence of flow in the irradiated vessels. (c) With IF-IBDV method, a similar functional map of the vasculature is obtained, with enhanced visualization of smaller arteriolar, venules, and capillaries. The scale bars in (a) represent 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368711&req=5

g008: Comparison of LSI and IF-IBDV images of microvascular network in DSWC model, after laser irradiation of select vessels. (a) With green filtering of broadband light used to transilluminate the DSWC, the blood-vessel architecture is clearly visible. (b) With LSI, vessels containing flowing blood are displayed, with an absence of flow in the irradiated vessels. (c) With IF-IBDV method, a similar functional map of the vasculature is obtained, with enhanced visualization of smaller arteriolar, venules, and capillaries. The scale bars in (a) represent 1 mm.
Mentions: Laser irradiation was then performed on the subdermal side of the DSWC. Blood vessels were irradiated with a sequence of three pulses from a frequency-doubled Nd:YAG laser (Dualis VP+, Fotona Laser, Ljublijana, Slovenia) with pulse width of 1 millisecond (ms), energy density of 5 J/cm2, repetition rate of 20 Hz, and beam sport size of 2 mm. To verify the OCT IF-IBDV results, we also took transillumination images with a green color filter [Fig. 8(a)Fig. 8

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

Show MeSH
Related in: MedlinePlus