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High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

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Related in: MedlinePlus

The IF-IBDV images of a flow phantom. (a) OCT image of a flow phantom. (b) IF-IBDV images of the flow phantom with the syringe pump stopped. (c) The histogram of the IBDV value distribution inside the tube in (b). (d) IF-IBDV images of the flow phantom with flow rate of 92.4 mm/s. (e) The histogram of the IBDV value distribution inside the tube in (d) . The scale bars in (a) represent 500 µm.
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g006: The IF-IBDV images of a flow phantom. (a) OCT image of a flow phantom. (b) IF-IBDV images of the flow phantom with the syringe pump stopped. (c) The histogram of the IBDV value distribution inside the tube in (b). (d) IF-IBDV images of the flow phantom with flow rate of 92.4 mm/s. (e) The histogram of the IBDV value distribution inside the tube in (d) . The scale bars in (a) represent 500 µm.

Mentions: These findings suggested that the ways to improve the blood vessel detection sensitivity for the phase resolved methods could also be used to improve the sensitivity of the IBDV method. This means the use of the algorithm along the slowing scanning direction (inter-frame algorithm) and the using of dual beam setup can also been used to improve the sensitivity of the IBDV method. Because the dual beam setup requires hardware modification of the system, we only tested the inter-frame algorithm and Fig. 6Fig. 6


High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

The IF-IBDV images of a flow phantom. (a) OCT image of a flow phantom. (b) IF-IBDV images of the flow phantom with the syringe pump stopped. (c) The histogram of the IBDV value distribution inside the tube in (b). (d) IF-IBDV images of the flow phantom with flow rate of 92.4 mm/s. (e) The histogram of the IBDV value distribution inside the tube in (d) . The scale bars in (a) represent 500 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368711&req=5

g006: The IF-IBDV images of a flow phantom. (a) OCT image of a flow phantom. (b) IF-IBDV images of the flow phantom with the syringe pump stopped. (c) The histogram of the IBDV value distribution inside the tube in (b). (d) IF-IBDV images of the flow phantom with flow rate of 92.4 mm/s. (e) The histogram of the IBDV value distribution inside the tube in (d) . The scale bars in (a) represent 500 µm.
Mentions: These findings suggested that the ways to improve the blood vessel detection sensitivity for the phase resolved methods could also be used to improve the sensitivity of the IBDV method. This means the use of the algorithm along the slowing scanning direction (inter-frame algorithm) and the using of dual beam setup can also been used to improve the sensitivity of the IBDV method. Because the dual beam setup requires hardware modification of the system, we only tested the inter-frame algorithm and Fig. 6Fig. 6

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

Show MeSH
Related in: MedlinePlus