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High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

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The dependence of IBDV value on neighboring A-line time interval. (a) OCT image of a flow phantom. (b)-(g) IBDV images of the flow phantom at different neighboring A-line time interval. The neighboring A-line time intervals are shown in the figures. (h) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. (i) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. The scale bars in (a) represent 500 µm.
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g005: The dependence of IBDV value on neighboring A-line time interval. (a) OCT image of a flow phantom. (b)-(g) IBDV images of the flow phantom at different neighboring A-line time interval. The neighboring A-line time intervals are shown in the figures. (h) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. (i) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. The scale bars in (a) represent 500 µm.

Mentions: Recently, high sensitivity imaging of microvasculature has been demonstrated with OCT by several groups [16–19]. For phase resolved DOCT, the blood flow sensitivity could be improved by increasing the time intervals between neighboring A-lines used in the algorithm. This can be realized by reducing the A-line rate of the system, by using the algorithm along the slowing scanning direction or by using dual beam setup [16–19]. Here, the effect of time interval between neighboring A-lines used in the IBDV algorithm on the sensitivity of IBDV was investigated and the results are shown in Fig. 5Fig. 5


High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.

Liu G, Jia W, Sun V, Choi B, Chen Z - Opt Express (2012)

The dependence of IBDV value on neighboring A-line time interval. (a) OCT image of a flow phantom. (b)-(g) IBDV images of the flow phantom at different neighboring A-line time interval. The neighboring A-line time intervals are shown in the figures. (h) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. (i) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. The scale bars in (a) represent 500 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368711&req=5

g005: The dependence of IBDV value on neighboring A-line time interval. (a) OCT image of a flow phantom. (b)-(g) IBDV images of the flow phantom at different neighboring A-line time interval. The neighboring A-line time intervals are shown in the figures. (h) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. (i) The relationship between average IBDV value in the tube and neighboring A-line time interval. The red line shows the exponential decay fit of the data. The scale bars in (a) represent 500 µm.
Mentions: Recently, high sensitivity imaging of microvasculature has been demonstrated with OCT by several groups [16–19]. For phase resolved DOCT, the blood flow sensitivity could be improved by increasing the time intervals between neighboring A-lines used in the algorithm. This can be realized by reducing the A-line rate of the system, by using the algorithm along the slowing scanning direction or by using dual beam setup [16–19]. Here, the effect of time interval between neighboring A-lines used in the IBDV algorithm on the sensitivity of IBDV was investigated and the results are shown in Fig. 5Fig. 5

Bottom Line: The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated.In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow.These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com

ABSTRACT
In this paper, the features of the intensity-based Doppler variance (IBDV) method were analyzed systemically with a flow phantom. The effects of beam scanning density, flow rate and the time interval between neighboring A-lines on the performance of this method were investigated. The IBDV method can be used to quantify the flow rate and its sensitivity can be improved by increasing the time interval between the neighboring A-lines. A higher sensitivity IBDV method that applies the algorithm along the slower scan direction was proposed. In comparison to laser speckle imaging maps of blood flow, we demonstrated the ability of the method to identify vessels with altered blood flow. In clinical measurements, we demonstrated the ability of the method to image vascular networks with exquisite spatial resolution and at depths up to 1.2 mm in human skin. These results collectively demonstrated the potential of the method to monitor the microvasculature during disease progression and in response to therapeutic intervention.

Show MeSH
Related in: MedlinePlus