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Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats.

Sintara K, Thong-Ngam D, Patumraj S, Klaikeaw N - J. Biomed. Biotechnol. (2012)

Bottom Line: The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO.Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl.Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
To determine effects of curcumin on N-methyl-N-nitrosourea (MNU) and saturated sodium chloride (s-NaCl)-induced gastric cancer in rats. Male Wistar rats were divided into 5 groups: control (CO), control supplemented with 200 mg/kg curcumin (CC), MNU + s-NaCl, MNU + s-NaCl supplemented with 200 mg/kg curcumin daily for the first 3 weeks (MNU + s-NaCl + C3W), and MNU + s-NaCl supplemented with curcumin for 20 weeks (MNU + s-NaCl + C20W). To induce stomach cancer, rats except for CO and CC were orally treated with 100 mg/kg MNU on day 0 and 14, and s-NaCl twice-a-week for the first 3 weeks. The experiment was finished and rats were sacrificed at the end of 20 weeks. Cancers were found in forestomachs of all rats in MNU + s-NaCl. The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO. Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin treatment for 20 weeks also decreased 8-OHdG expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

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Immunohistochemical staining of 8-OHdG antibodies in the representative tissue specimens (400x): CO group (a), MNU + s-NaCl group (b), C20W cancer group (c), and C20W benign group (d). DAB stained immunoreactive cells (dark brown, arrows); nuclear counterstaining was performed with Hematoxylin II and Bluing reagent. LP: laminar propria and MM: muscularis mucosae.
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fig3: Immunohistochemical staining of 8-OHdG antibodies in the representative tissue specimens (400x): CO group (a), MNU + s-NaCl group (b), C20W cancer group (c), and C20W benign group (d). DAB stained immunoreactive cells (dark brown, arrows); nuclear counterstaining was performed with Hematoxylin II and Bluing reagent. LP: laminar propria and MM: muscularis mucosae.

Mentions: 8-OHdG and cyclin D1 expressions were studied by immunohistochemistry and shown as nuclei-stained cells. The average percentages of immunoreactive cells of all groups were shown in Table 2. From the results, the mean percentages of 8-OHdG and cyclin D1-immunoreactive cells were not significantly different between CO and CC. They were significantly increased in MNU + s-NaCl compared with CO (P = 0.002 and P = 0.000, resp.). Curcumin supplementation for 20 weeks reduced gastric cancer incidence and significantly decreased 8-OHdG expression in C20W benign group compared with MNU + s-NaCl (P = 0.015) (Table 2). The average percentage of cyclin D1 stained cells in the C20W benign group tended to reduce, but not reach a statistically significant level when compared with MNU + s-NaCl (P = 0.062; Table 2). 8-OHdG was expressed primarily in gastric epithelial cells (Figure 2(a)). This expression was increased in the mucosa of SCC in both MNU + s-NaCl and C20W cancer groups (Figures 3(b) and 3(c)). This enhanced expression was limited to a small number of epithelial cells in C20W benign group (Figure 3(d)).


Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats.

Sintara K, Thong-Ngam D, Patumraj S, Klaikeaw N - J. Biomed. Biotechnol. (2012)

Immunohistochemical staining of 8-OHdG antibodies in the representative tissue specimens (400x): CO group (a), MNU + s-NaCl group (b), C20W cancer group (c), and C20W benign group (d). DAB stained immunoreactive cells (dark brown, arrows); nuclear counterstaining was performed with Hematoxylin II and Bluing reagent. LP: laminar propria and MM: muscularis mucosae.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368696&req=5

fig3: Immunohistochemical staining of 8-OHdG antibodies in the representative tissue specimens (400x): CO group (a), MNU + s-NaCl group (b), C20W cancer group (c), and C20W benign group (d). DAB stained immunoreactive cells (dark brown, arrows); nuclear counterstaining was performed with Hematoxylin II and Bluing reagent. LP: laminar propria and MM: muscularis mucosae.
Mentions: 8-OHdG and cyclin D1 expressions were studied by immunohistochemistry and shown as nuclei-stained cells. The average percentages of immunoreactive cells of all groups were shown in Table 2. From the results, the mean percentages of 8-OHdG and cyclin D1-immunoreactive cells were not significantly different between CO and CC. They were significantly increased in MNU + s-NaCl compared with CO (P = 0.002 and P = 0.000, resp.). Curcumin supplementation for 20 weeks reduced gastric cancer incidence and significantly decreased 8-OHdG expression in C20W benign group compared with MNU + s-NaCl (P = 0.015) (Table 2). The average percentage of cyclin D1 stained cells in the C20W benign group tended to reduce, but not reach a statistically significant level when compared with MNU + s-NaCl (P = 0.062; Table 2). 8-OHdG was expressed primarily in gastric epithelial cells (Figure 2(a)). This expression was increased in the mucosa of SCC in both MNU + s-NaCl and C20W cancer groups (Figures 3(b) and 3(c)). This enhanced expression was limited to a small number of epithelial cells in C20W benign group (Figure 3(d)).

Bottom Line: The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO.Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl.Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
To determine effects of curcumin on N-methyl-N-nitrosourea (MNU) and saturated sodium chloride (s-NaCl)-induced gastric cancer in rats. Male Wistar rats were divided into 5 groups: control (CO), control supplemented with 200 mg/kg curcumin (CC), MNU + s-NaCl, MNU + s-NaCl supplemented with 200 mg/kg curcumin daily for the first 3 weeks (MNU + s-NaCl + C3W), and MNU + s-NaCl supplemented with curcumin for 20 weeks (MNU + s-NaCl + C20W). To induce stomach cancer, rats except for CO and CC were orally treated with 100 mg/kg MNU on day 0 and 14, and s-NaCl twice-a-week for the first 3 weeks. The experiment was finished and rats were sacrificed at the end of 20 weeks. Cancers were found in forestomachs of all rats in MNU + s-NaCl. The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO. Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin treatment for 20 weeks also decreased 8-OHdG expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

Show MeSH
Related in: MedlinePlus