Limits...
Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats.

Sintara K, Thong-Ngam D, Patumraj S, Klaikeaw N - J. Biomed. Biotechnol. (2012)

Bottom Line: The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO.Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl.Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
To determine effects of curcumin on N-methyl-N-nitrosourea (MNU) and saturated sodium chloride (s-NaCl)-induced gastric cancer in rats. Male Wistar rats were divided into 5 groups: control (CO), control supplemented with 200 mg/kg curcumin (CC), MNU + s-NaCl, MNU + s-NaCl supplemented with 200 mg/kg curcumin daily for the first 3 weeks (MNU + s-NaCl + C3W), and MNU + s-NaCl supplemented with curcumin for 20 weeks (MNU + s-NaCl + C20W). To induce stomach cancer, rats except for CO and CC were orally treated with 100 mg/kg MNU on day 0 and 14, and s-NaCl twice-a-week for the first 3 weeks. The experiment was finished and rats were sacrificed at the end of 20 weeks. Cancers were found in forestomachs of all rats in MNU + s-NaCl. The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO. Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin treatment for 20 weeks also decreased 8-OHdG expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

Show MeSH

Related in: MedlinePlus

Western blot analysis of phospho-IκBα expression. Curcumin supplementation in C3W benign group declined the expression of phosphorylated IκBα compared with MNU + s-NaCl (a), whereas the expression of phosphorylated IκBα in C3W cancer group did not decrease (b). Curcumin supplementation for 20 weeks in C20W benign decreased the expression of phosphorylated IκBα compared with MNU + s-NaCl (c), whereas the expression of phosphorylated IκBα in C20W cancer group did not decline (d). An antibody for β-actin was used as an internal control.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368696&req=5

fig2: Western blot analysis of phospho-IκBα expression. Curcumin supplementation in C3W benign group declined the expression of phosphorylated IκBα compared with MNU + s-NaCl (a), whereas the expression of phosphorylated IκBα in C3W cancer group did not decrease (b). Curcumin supplementation for 20 weeks in C20W benign decreased the expression of phosphorylated IκBα compared with MNU + s-NaCl (c), whereas the expression of phosphorylated IκBα in C20W cancer group did not decline (d). An antibody for β-actin was used as an internal control.

Mentions: From histopathological results (Table 1), rats in MNU + s-NaCl + C3W and MNU + s-NaCl + C20W groups were divided into subgroup C3W benign group (N = 2), C3W cancer group (N = 3), C20W benign group (N = 3), and C20W cancer group (N = 3). Results showed that the expression of phosphorylated IκBα was not significantly different between CO and CC. Compared with CO, phosphorylated IκBα expression in MNU + s-NaCl increased significantly (P = 0.010). Curcumin supplementations for 3 and 20 weeks in C3W benign and C20W benign groups significantly declined the expression of phosphorylated IκBα compared with MNU + s-NaCl (P = 0.028 and P = 0.031, resp.) (Table 2). The represented bands of phospho-IκBα and β-actin were shown in Figure 2.


Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats.

Sintara K, Thong-Ngam D, Patumraj S, Klaikeaw N - J. Biomed. Biotechnol. (2012)

Western blot analysis of phospho-IκBα expression. Curcumin supplementation in C3W benign group declined the expression of phosphorylated IκBα compared with MNU + s-NaCl (a), whereas the expression of phosphorylated IκBα in C3W cancer group did not decrease (b). Curcumin supplementation for 20 weeks in C20W benign decreased the expression of phosphorylated IκBα compared with MNU + s-NaCl (c), whereas the expression of phosphorylated IκBα in C20W cancer group did not decline (d). An antibody for β-actin was used as an internal control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368696&req=5

fig2: Western blot analysis of phospho-IκBα expression. Curcumin supplementation in C3W benign group declined the expression of phosphorylated IκBα compared with MNU + s-NaCl (a), whereas the expression of phosphorylated IκBα in C3W cancer group did not decrease (b). Curcumin supplementation for 20 weeks in C20W benign decreased the expression of phosphorylated IκBα compared with MNU + s-NaCl (c), whereas the expression of phosphorylated IκBα in C20W cancer group did not decline (d). An antibody for β-actin was used as an internal control.
Mentions: From histopathological results (Table 1), rats in MNU + s-NaCl + C3W and MNU + s-NaCl + C20W groups were divided into subgroup C3W benign group (N = 2), C3W cancer group (N = 3), C20W benign group (N = 3), and C20W cancer group (N = 3). Results showed that the expression of phosphorylated IκBα was not significantly different between CO and CC. Compared with CO, phosphorylated IκBα expression in MNU + s-NaCl increased significantly (P = 0.010). Curcumin supplementations for 3 and 20 weeks in C3W benign and C20W benign groups significantly declined the expression of phosphorylated IκBα compared with MNU + s-NaCl (P = 0.028 and P = 0.031, resp.) (Table 2). The represented bands of phospho-IκBα and β-actin were shown in Figure 2.

Bottom Line: The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO.Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl.Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
To determine effects of curcumin on N-methyl-N-nitrosourea (MNU) and saturated sodium chloride (s-NaCl)-induced gastric cancer in rats. Male Wistar rats were divided into 5 groups: control (CO), control supplemented with 200 mg/kg curcumin (CC), MNU + s-NaCl, MNU + s-NaCl supplemented with 200 mg/kg curcumin daily for the first 3 weeks (MNU + s-NaCl + C3W), and MNU + s-NaCl supplemented with curcumin for 20 weeks (MNU + s-NaCl + C20W). To induce stomach cancer, rats except for CO and CC were orally treated with 100 mg/kg MNU on day 0 and 14, and s-NaCl twice-a-week for the first 3 weeks. The experiment was finished and rats were sacrificed at the end of 20 weeks. Cancers were found in forestomachs of all rats in MNU + s-NaCl. The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO. Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin treatment for 20 weeks also decreased 8-OHdG expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

Show MeSH
Related in: MedlinePlus