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Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats.

Sintara K, Thong-Ngam D, Patumraj S, Klaikeaw N - J. Biomed. Biotechnol. (2012)

Bottom Line: The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO.Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl.Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
To determine effects of curcumin on N-methyl-N-nitrosourea (MNU) and saturated sodium chloride (s-NaCl)-induced gastric cancer in rats. Male Wistar rats were divided into 5 groups: control (CO), control supplemented with 200 mg/kg curcumin (CC), MNU + s-NaCl, MNU + s-NaCl supplemented with 200 mg/kg curcumin daily for the first 3 weeks (MNU + s-NaCl + C3W), and MNU + s-NaCl supplemented with curcumin for 20 weeks (MNU + s-NaCl + C20W). To induce stomach cancer, rats except for CO and CC were orally treated with 100 mg/kg MNU on day 0 and 14, and s-NaCl twice-a-week for the first 3 weeks. The experiment was finished and rats were sacrificed at the end of 20 weeks. Cancers were found in forestomachs of all rats in MNU + s-NaCl. The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO. Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin treatment for 20 weeks also decreased 8-OHdG expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

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Macroscopic (a) and microscopic (b–d, H&E staining, 40x) appearance of squamous cell carcinoma (SCC) in the forestomachs. The multiple polypoid tumors developed in the stomach after MNU and saturated NaCl administration (a). SCCs in the gastric epithelium show the invasion of cancerous tissue with dyskeratosis through submucosal layer (b), muscle layer (c), or serosa (d). Note: the arrows indicate SCC with submucosal invasion (b), muscle-layer invasion (c), and stomach perforation (d). MM: muscularis mucosae, SM: submucosa, and M: muscle layer.
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fig1: Macroscopic (a) and microscopic (b–d, H&E staining, 40x) appearance of squamous cell carcinoma (SCC) in the forestomachs. The multiple polypoid tumors developed in the stomach after MNU and saturated NaCl administration (a). SCCs in the gastric epithelium show the invasion of cancerous tissue with dyskeratosis through submucosal layer (b), muscle layer (c), or serosa (d). Note: the arrows indicate SCC with submucosal invasion (b), muscle-layer invasion (c), and stomach perforation (d). MM: muscularis mucosae, SM: submucosa, and M: muscle layer.

Mentions: Gastric cancer incidence was shown in Table 1. There was no squamous cell carcinoma (SCC) in CO and CC groups. In MNU + s-NaCl group, SCC was found in forestomach of all rats (100%). SCC showed tumors scattered in the forestomach. In gross view, the tumor masses were coliform-like (Figure 1(a)). Histopathology of SCC showed dyskeratosis and invasion of cancerous tissue through all layers of stomach wall. The cancerous tissue invaded the submucosal layer (Figure 1(b)), the muscle layer (Figure 1(c)), or the serosa (Figure 1(d)). 3/5 (60%) rats in MNU + s-NaCl + C3W developed SCC. 2 rats in this group showed tumor-like lesions. Papillary growth or tumor-like lesion displayed hyperproliferation of epithelial cells, hyperkeratosis, but no invasion. These lesions were not diagnosed as cancers. In MNU + s-NaCl + C20W group, three rats developed SCC with submucosal invasion. The cancer incidence was 50%. One rat did not exhibit a cancerous lesion. Another 2 rats developed papillary growths.


Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats.

Sintara K, Thong-Ngam D, Patumraj S, Klaikeaw N - J. Biomed. Biotechnol. (2012)

Macroscopic (a) and microscopic (b–d, H&E staining, 40x) appearance of squamous cell carcinoma (SCC) in the forestomachs. The multiple polypoid tumors developed in the stomach after MNU and saturated NaCl administration (a). SCCs in the gastric epithelium show the invasion of cancerous tissue with dyskeratosis through submucosal layer (b), muscle layer (c), or serosa (d). Note: the arrows indicate SCC with submucosal invasion (b), muscle-layer invasion (c), and stomach perforation (d). MM: muscularis mucosae, SM: submucosa, and M: muscle layer.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368696&req=5

fig1: Macroscopic (a) and microscopic (b–d, H&E staining, 40x) appearance of squamous cell carcinoma (SCC) in the forestomachs. The multiple polypoid tumors developed in the stomach after MNU and saturated NaCl administration (a). SCCs in the gastric epithelium show the invasion of cancerous tissue with dyskeratosis through submucosal layer (b), muscle layer (c), or serosa (d). Note: the arrows indicate SCC with submucosal invasion (b), muscle-layer invasion (c), and stomach perforation (d). MM: muscularis mucosae, SM: submucosa, and M: muscle layer.
Mentions: Gastric cancer incidence was shown in Table 1. There was no squamous cell carcinoma (SCC) in CO and CC groups. In MNU + s-NaCl group, SCC was found in forestomach of all rats (100%). SCC showed tumors scattered in the forestomach. In gross view, the tumor masses were coliform-like (Figure 1(a)). Histopathology of SCC showed dyskeratosis and invasion of cancerous tissue through all layers of stomach wall. The cancerous tissue invaded the submucosal layer (Figure 1(b)), the muscle layer (Figure 1(c)), or the serosa (Figure 1(d)). 3/5 (60%) rats in MNU + s-NaCl + C3W developed SCC. 2 rats in this group showed tumor-like lesions. Papillary growth or tumor-like lesion displayed hyperproliferation of epithelial cells, hyperkeratosis, but no invasion. These lesions were not diagnosed as cancers. In MNU + s-NaCl + C20W group, three rats developed SCC with submucosal invasion. The cancer incidence was 50%. One rat did not exhibit a cancerous lesion. Another 2 rats developed papillary growths.

Bottom Line: The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO.Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl.Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
To determine effects of curcumin on N-methyl-N-nitrosourea (MNU) and saturated sodium chloride (s-NaCl)-induced gastric cancer in rats. Male Wistar rats were divided into 5 groups: control (CO), control supplemented with 200 mg/kg curcumin (CC), MNU + s-NaCl, MNU + s-NaCl supplemented with 200 mg/kg curcumin daily for the first 3 weeks (MNU + s-NaCl + C3W), and MNU + s-NaCl supplemented with curcumin for 20 weeks (MNU + s-NaCl + C20W). To induce stomach cancer, rats except for CO and CC were orally treated with 100 mg/kg MNU on day 0 and 14, and s-NaCl twice-a-week for the first 3 weeks. The experiment was finished and rats were sacrificed at the end of 20 weeks. Cancers were found in forestomachs of all rats in MNU + s-NaCl. The expressions of phosphorylated inhibitor kappaB alpha (phospho-IκBα), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and cyclin D1 significantly increased in MNU + s-NaCl compared with CO. Curcumin treatments for 3 and 20 weeks reduced the cancer incidence resulting in a decrease of phospho-IκBα expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin treatment for 20 weeks also decreased 8-OHdG expression in benign tumor-bearing rats compared with MNU + s-NaCl. Curcumin can attenuate cancer via a reduction of phospho-IκBα and 8-OHdG expressions, which may play a promising role in gastric carcinogenesis.

Show MeSH
Related in: MedlinePlus