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Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells.

Wang Y, Zi XY, Su J, Zhang HX, Zhang XR, Zhu HY, Li JX, Yin M, Yang F, Hu YP - Int J Nanomedicine (2012)

Bottom Line: In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects.Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis.CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects. Cuprous oxide nanoparticles (CONPs) can selectively induce apoptosis and suppress the proliferation of tumor cells, showing great potential as a clinical cancer therapy. Treatment with CONPs caused a G1/G0 cell cycle arrest in tumor cells. Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis. CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells. Our results demonstrate that CONPs have selective cytotoxicity towards tumor cells, and indicate that CONPs might be a potential nanomedicine for cancer therapy.

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DTT protection experiment on HeLa cells for 48 hours. The MTT assay was then performed to evaluate protective effects of DTT.Notes: The results show that DTT could increase cell viability at low concentrations but reduced viability at higher concentrations (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; MTT, 3-(4,5-di-methylthiazol-2yl)-2,5-diphenyl tetrazolium bromide.
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f9-ijn-7-2641: DTT protection experiment on HeLa cells for 48 hours. The MTT assay was then performed to evaluate protective effects of DTT.Notes: The results show that DTT could increase cell viability at low concentrations but reduced viability at higher concentrations (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; MTT, 3-(4,5-di-methylthiazol-2yl)-2,5-diphenyl tetrazolium bromide.

Mentions: ROS have a very close relationship with various vital movements and participate in immunological and pathological reactions, cell injury and death, and tumor generation. Recent researches have shown that metal oxide nanoparticles can exhibit spontaneous or light-activated ROS production, depending on the material composition and surface characteristics.15,36 To determine the role of ROS in cell death, DTT protection experiments and lipid peroxidation measurements were performed. DTT is commonly used in biochemical studies to prevent the oxidation of thiol groups and to reduce disulfides to dithiols, which can inactivate ROS.37–39 Our study results show that DTT could significantly prevent the cytotoxicity observed in cells treated with low concentrations of CONPs. However, DTT was unable to prevent significantly the cytotoxicity observed at higher concentrations (P < 0.01) (Figure 9). These studies indicate that the pharmacological actions of CONPs might result from the generation of ROS, which in turn are responsible for inducing apoptosis.


Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells.

Wang Y, Zi XY, Su J, Zhang HX, Zhang XR, Zhu HY, Li JX, Yin M, Yang F, Hu YP - Int J Nanomedicine (2012)

DTT protection experiment on HeLa cells for 48 hours. The MTT assay was then performed to evaluate protective effects of DTT.Notes: The results show that DTT could increase cell viability at low concentrations but reduced viability at higher concentrations (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; MTT, 3-(4,5-di-methylthiazol-2yl)-2,5-diphenyl tetrazolium bromide.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368515&req=5

f9-ijn-7-2641: DTT protection experiment on HeLa cells for 48 hours. The MTT assay was then performed to evaluate protective effects of DTT.Notes: The results show that DTT could increase cell viability at low concentrations but reduced viability at higher concentrations (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; MTT, 3-(4,5-di-methylthiazol-2yl)-2,5-diphenyl tetrazolium bromide.
Mentions: ROS have a very close relationship with various vital movements and participate in immunological and pathological reactions, cell injury and death, and tumor generation. Recent researches have shown that metal oxide nanoparticles can exhibit spontaneous or light-activated ROS production, depending on the material composition and surface characteristics.15,36 To determine the role of ROS in cell death, DTT protection experiments and lipid peroxidation measurements were performed. DTT is commonly used in biochemical studies to prevent the oxidation of thiol groups and to reduce disulfides to dithiols, which can inactivate ROS.37–39 Our study results show that DTT could significantly prevent the cytotoxicity observed in cells treated with low concentrations of CONPs. However, DTT was unable to prevent significantly the cytotoxicity observed at higher concentrations (P < 0.01) (Figure 9). These studies indicate that the pharmacological actions of CONPs might result from the generation of ROS, which in turn are responsible for inducing apoptosis.

Bottom Line: In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects.Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis.CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects. Cuprous oxide nanoparticles (CONPs) can selectively induce apoptosis and suppress the proliferation of tumor cells, showing great potential as a clinical cancer therapy. Treatment with CONPs caused a G1/G0 cell cycle arrest in tumor cells. Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis. CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells. Our results demonstrate that CONPs have selective cytotoxicity towards tumor cells, and indicate that CONPs might be a potential nanomedicine for cancer therapy.

Show MeSH
Related in: MedlinePlus