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Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells.

Wang Y, Zi XY, Su J, Zhang HX, Zhang XR, Zhu HY, Li JX, Yin M, Yang F, Hu YP - Int J Nanomedicine (2012)

Bottom Line: In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects.Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis.CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects. Cuprous oxide nanoparticles (CONPs) can selectively induce apoptosis and suppress the proliferation of tumor cells, showing great potential as a clinical cancer therapy. Treatment with CONPs caused a G1/G0 cell cycle arrest in tumor cells. Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis. CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells. Our results demonstrate that CONPs have selective cytotoxicity towards tumor cells, and indicate that CONPs might be a potential nanomedicine for cancer therapy.

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Flow cytometry analysis of HeLa and MEF cells treated with different concentrations of CONPs for 48 hours and then stained with Annexin V and PI.Notes: Significant increases in apoptosis were observed in HeLa cells exposed to the 10 μg/mL and 20 μg/mL CONPs for 48 hours. However, decreased apoptosis was observed in the MEF cells. The upper-left quadrant in each panel shows the necrotic population, the upper-right quadrant shows the late apoptotic population, and the lower-right quadrant shows the early apoptotic population.Abbreviations: CONPs, cuprous oxide nanoparticles; MEF, mouse embryonic fibroblast; PI, propidium iodine.
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f6-ijn-7-2641: Flow cytometry analysis of HeLa and MEF cells treated with different concentrations of CONPs for 48 hours and then stained with Annexin V and PI.Notes: Significant increases in apoptosis were observed in HeLa cells exposed to the 10 μg/mL and 20 μg/mL CONPs for 48 hours. However, decreased apoptosis was observed in the MEF cells. The upper-left quadrant in each panel shows the necrotic population, the upper-right quadrant shows the late apoptotic population, and the lower-right quadrant shows the early apoptotic population.Abbreviations: CONPs, cuprous oxide nanoparticles; MEF, mouse embryonic fibroblast; PI, propidium iodine.

Mentions: Several studies have indicated that different nanoparticles can suppress cell viability by different mechanisms. Specific kinds of nanoparticles can induce apoptosis in cells, but other kinds of nanoparticles can make cells undergo necrosis.15,16,31 To investigate the mechanism of CONPs cytotoxicity, we performed flow cytometry to analyze the effects of CONPs on cell death and cell cycle. First, Annexin V- and PI-based apoptosis and necrosis discrimination assays were performed on HeLa and MEF cells exposed to 10 μg/mL and 20 μg/mL of CONPs for 48 hours (Figure 6). The results showed an increase in apoptosis in HeLa cells, which was dependent on the dose of CONPs, and a similar increase in necrosis was observed. However, decreased apoptosis and necrosis were observed in the MEF cells in accordance with the cytotoxicity assays. These results showed that CONPs have selective antitumor properties.


Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells.

Wang Y, Zi XY, Su J, Zhang HX, Zhang XR, Zhu HY, Li JX, Yin M, Yang F, Hu YP - Int J Nanomedicine (2012)

Flow cytometry analysis of HeLa and MEF cells treated with different concentrations of CONPs for 48 hours and then stained with Annexin V and PI.Notes: Significant increases in apoptosis were observed in HeLa cells exposed to the 10 μg/mL and 20 μg/mL CONPs for 48 hours. However, decreased apoptosis was observed in the MEF cells. The upper-left quadrant in each panel shows the necrotic population, the upper-right quadrant shows the late apoptotic population, and the lower-right quadrant shows the early apoptotic population.Abbreviations: CONPs, cuprous oxide nanoparticles; MEF, mouse embryonic fibroblast; PI, propidium iodine.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3368515&req=5

f6-ijn-7-2641: Flow cytometry analysis of HeLa and MEF cells treated with different concentrations of CONPs for 48 hours and then stained with Annexin V and PI.Notes: Significant increases in apoptosis were observed in HeLa cells exposed to the 10 μg/mL and 20 μg/mL CONPs for 48 hours. However, decreased apoptosis was observed in the MEF cells. The upper-left quadrant in each panel shows the necrotic population, the upper-right quadrant shows the late apoptotic population, and the lower-right quadrant shows the early apoptotic population.Abbreviations: CONPs, cuprous oxide nanoparticles; MEF, mouse embryonic fibroblast; PI, propidium iodine.
Mentions: Several studies have indicated that different nanoparticles can suppress cell viability by different mechanisms. Specific kinds of nanoparticles can induce apoptosis in cells, but other kinds of nanoparticles can make cells undergo necrosis.15,16,31 To investigate the mechanism of CONPs cytotoxicity, we performed flow cytometry to analyze the effects of CONPs on cell death and cell cycle. First, Annexin V- and PI-based apoptosis and necrosis discrimination assays were performed on HeLa and MEF cells exposed to 10 μg/mL and 20 μg/mL of CONPs for 48 hours (Figure 6). The results showed an increase in apoptosis in HeLa cells, which was dependent on the dose of CONPs, and a similar increase in necrosis was observed. However, decreased apoptosis and necrosis were observed in the MEF cells in accordance with the cytotoxicity assays. These results showed that CONPs have selective antitumor properties.

Bottom Line: In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects.Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis.CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects. Cuprous oxide nanoparticles (CONPs) can selectively induce apoptosis and suppress the proliferation of tumor cells, showing great potential as a clinical cancer therapy. Treatment with CONPs caused a G1/G0 cell cycle arrest in tumor cells. Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis. CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells. Our results demonstrate that CONPs have selective cytotoxicity towards tumor cells, and indicate that CONPs might be a potential nanomedicine for cancer therapy.

Show MeSH
Related in: MedlinePlus