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Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells.

Wang Y, Zi XY, Su J, Zhang HX, Zhang XR, Zhu HY, Li JX, Yin M, Yang F, Hu YP - Int J Nanomedicine (2012)

Bottom Line: In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects.Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis.CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects. Cuprous oxide nanoparticles (CONPs) can selectively induce apoptosis and suppress the proliferation of tumor cells, showing great potential as a clinical cancer therapy. Treatment with CONPs caused a G1/G0 cell cycle arrest in tumor cells. Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis. CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells. Our results demonstrate that CONPs have selective cytotoxicity towards tumor cells, and indicate that CONPs might be a potential nanomedicine for cancer therapy.

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Related in: MedlinePlus

Lipid peroxidation measurement of CONPs in the liposomes prepared from soybean lecithin and cholesterol.Notes: CONPs increased the lipid peroxidation activity and produced ROS, but DTT could significantly block the generation of ROS (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; ROS, reactive oxygen species.
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f10-ijn-7-2641: Lipid peroxidation measurement of CONPs in the liposomes prepared from soybean lecithin and cholesterol.Notes: CONPs increased the lipid peroxidation activity and produced ROS, but DTT could significantly block the generation of ROS (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; ROS, reactive oxygen species.

Mentions: To highlight ROS production, we performed lipid peroxidation experiments. The oxidation of fatty acids leads to the generation of lipid peroxides, thereby initiating a chain reaction that results in the disruption of the plasma and organelle membranes and subsequent cell death. Lipid peroxidation experiments show that DTT effectively inhibited the generation of the lipid peroxidation product malondialdehyde (MDA). MDA could be observed at low levels only when a concentration of greater than 20 μg/mL CONPs was used (Figure 10). However, higher levels of MDA were observed when DTT was not in the reaction mixture.


Cuprous oxide nanoparticles selectively induce apoptosis of tumor cells.

Wang Y, Zi XY, Su J, Zhang HX, Zhang XR, Zhu HY, Li JX, Yin M, Yang F, Hu YP - Int J Nanomedicine (2012)

Lipid peroxidation measurement of CONPs in the liposomes prepared from soybean lecithin and cholesterol.Notes: CONPs increased the lipid peroxidation activity and produced ROS, but DTT could significantly block the generation of ROS (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; ROS, reactive oxygen species.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368515&req=5

f10-ijn-7-2641: Lipid peroxidation measurement of CONPs in the liposomes prepared from soybean lecithin and cholesterol.Notes: CONPs increased the lipid peroxidation activity and produced ROS, but DTT could significantly block the generation of ROS (P < 0.01, n = 3).Abbreviations: CONPs, cuprous oxide nanoparticles; DTT, dithiothreitol; ROS, reactive oxygen species.
Mentions: To highlight ROS production, we performed lipid peroxidation experiments. The oxidation of fatty acids leads to the generation of lipid peroxides, thereby initiating a chain reaction that results in the disruption of the plasma and organelle membranes and subsequent cell death. Lipid peroxidation experiments show that DTT effectively inhibited the generation of the lipid peroxidation product malondialdehyde (MDA). MDA could be observed at low levels only when a concentration of greater than 20 μg/mL CONPs was used (Figure 10). However, higher levels of MDA were observed when DTT was not in the reaction mixture.

Bottom Line: In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects.Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis.CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Second Military Medical University, Shanghai, People's Republic of China.

ABSTRACT
In the rapid development of nanoscience and nanotechnology, many researchers have discovered that metal oxide nanoparticles have very useful pharmacological effects. Cuprous oxide nanoparticles (CONPs) can selectively induce apoptosis and suppress the proliferation of tumor cells, showing great potential as a clinical cancer therapy. Treatment with CONPs caused a G1/G0 cell cycle arrest in tumor cells. Furthermore, CONPs enclosed in vesicles entered, or were taken up by mitochondria, which damaged their membranes, thereby inducing apoptosis. CONPs can also produce reactive oxygen species (ROS) and initiate lipid peroxidation of the liposomal membrane, thereby regulating many signaling pathways and influencing the vital movements of cells. Our results demonstrate that CONPs have selective cytotoxicity towards tumor cells, and indicate that CONPs might be a potential nanomedicine for cancer therapy.

Show MeSH
Related in: MedlinePlus