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In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse.

Terakawa M, Tsunoi Y, Mitsuhashi T - Int J Nanomedicine (2012)

Bottom Line: A polylactic acid (PLA) sphere, a biodegradable polymer, was used.Fluorescein isothiocyanate (FITC)-dextran and short interfering RNA were delivered into many human epithelial carcinoma cells (A431 cells) by applying a single 80 fs laser pulse in the presence of antibody-conjugated PLA microspheres.Perforation by biodegradable spheres compared with other particles has the potential to be a much safer phototherapy and drug delivery method for patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronics and Electrical Engineering, Keio University, Yokohama, Kanagawa, Japan. terakawa@elec.keio.ac.jp

ABSTRACT
Pulsed laser interaction with small metallic and dielectric particles has been receiving attention as a method of drug delivery to many cells. However, most of the particles are attended by many risks, which are mainly dependent upon particle size. Unlike other widely used particles, biodegradable particles have advantages of being broken down and eliminated by innate metabolic processes. In this paper, the perforation of cell membrane by a focused spot with transparent biodegradable microspheres excited by a single 800 nm, 80 fs laser pulse is demonstrated. A polylactic acid (PLA) sphere, a biodegradable polymer, was used. Fluorescein isothiocyanate (FITC)-dextran and short interfering RNA were delivered into many human epithelial carcinoma cells (A431 cells) by applying a single 80 fs laser pulse in the presence of antibody-conjugated PLA microspheres. The focused intensity was also simulated by the three-dimensional finite-difference time-domain method. Perforation by biodegradable spheres compared with other particles has the potential to be a much safer phototherapy and drug delivery method for patients. The present method can open a new avenue, which is considered an efficient adherent for the selective perforation of cells which express the specific antigen on the cell membrane.

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Related in: MedlinePlus

Phase contrast image of the A431 cells before laser illumination.Note: The polylactic acid spheres with a diameter of 2000 nm conjugated to the surface of the cells.
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f3-ijn-7-2653: Phase contrast image of the A431 cells before laser illumination.Note: The polylactic acid spheres with a diameter of 2000 nm conjugated to the surface of the cells.

Mentions: Figure 3 shows a phase contrast image of the A431 cells and the PLA microspheres taken before the laser illumination. As can be seen in the figure, the spheres of 2000 nm diameter conjugated to the A431 cells, which are 40–50 μm in size. The average number of spheres conjugated to the cell membrane is 51.


In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse.

Terakawa M, Tsunoi Y, Mitsuhashi T - Int J Nanomedicine (2012)

Phase contrast image of the A431 cells before laser illumination.Note: The polylactic acid spheres with a diameter of 2000 nm conjugated to the surface of the cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368514&req=5

f3-ijn-7-2653: Phase contrast image of the A431 cells before laser illumination.Note: The polylactic acid spheres with a diameter of 2000 nm conjugated to the surface of the cells.
Mentions: Figure 3 shows a phase contrast image of the A431 cells and the PLA microspheres taken before the laser illumination. As can be seen in the figure, the spheres of 2000 nm diameter conjugated to the A431 cells, which are 40–50 μm in size. The average number of spheres conjugated to the cell membrane is 51.

Bottom Line: A polylactic acid (PLA) sphere, a biodegradable polymer, was used.Fluorescein isothiocyanate (FITC)-dextran and short interfering RNA were delivered into many human epithelial carcinoma cells (A431 cells) by applying a single 80 fs laser pulse in the presence of antibody-conjugated PLA microspheres.Perforation by biodegradable spheres compared with other particles has the potential to be a much safer phototherapy and drug delivery method for patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronics and Electrical Engineering, Keio University, Yokohama, Kanagawa, Japan. terakawa@elec.keio.ac.jp

ABSTRACT
Pulsed laser interaction with small metallic and dielectric particles has been receiving attention as a method of drug delivery to many cells. However, most of the particles are attended by many risks, which are mainly dependent upon particle size. Unlike other widely used particles, biodegradable particles have advantages of being broken down and eliminated by innate metabolic processes. In this paper, the perforation of cell membrane by a focused spot with transparent biodegradable microspheres excited by a single 800 nm, 80 fs laser pulse is demonstrated. A polylactic acid (PLA) sphere, a biodegradable polymer, was used. Fluorescein isothiocyanate (FITC)-dextran and short interfering RNA were delivered into many human epithelial carcinoma cells (A431 cells) by applying a single 80 fs laser pulse in the presence of antibody-conjugated PLA microspheres. The focused intensity was also simulated by the three-dimensional finite-difference time-domain method. Perforation by biodegradable spheres compared with other particles has the potential to be a much safer phototherapy and drug delivery method for patients. The present method can open a new avenue, which is considered an efficient adherent for the selective perforation of cells which express the specific antigen on the cell membrane.

Show MeSH
Related in: MedlinePlus