Limits...
Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering.

Ibarretxe G, Alvarez A, CaƱavate ML, Hilario E, Aurrekoetxea M, Unda F - Stem Cells Int (2012)

Bottom Line: The potential of IPS cell technology is tremendous, but it will be essential to improve the methodologies for IPS cell generation and to precisely evaluate each clone and subclone of IPS cells for their safety and efficacy.Additionally, the current state of knowledge on IPS cells advises that research on their regenerative properties is carried out in appropriate tissue and organ systems that permit a safe assessment of the long-term behavior of these reprogrammed cells.In the present paper, we discuss the mechanisms of cell reprogramming, current technical limitations of IPS cells for their use in human tissue engineering, and possibilities to overcome them in the particular case of dental regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain.

ABSTRACT
The procurement of induced pluripotent stem cells, or IPS cells, from adult differentiated animal cells has the potential to revolutionize future medicine, where reprogrammed IPS cells may be used to repair disease-affected tissues on demand. The potential of IPS cell technology is tremendous, but it will be essential to improve the methodologies for IPS cell generation and to precisely evaluate each clone and subclone of IPS cells for their safety and efficacy. Additionally, the current state of knowledge on IPS cells advises that research on their regenerative properties is carried out in appropriate tissue and organ systems that permit a safe assessment of the long-term behavior of these reprogrammed cells. In the present paper, we discuss the mechanisms of cell reprogramming, current technical limitations of IPS cells for their use in human tissue engineering, and possibilities to overcome them in the particular case of dental regeneration.

No MeSH data available.


Basic scheme of tissue engineering. A biopsy is carried out to extract cells from the patient. These can be endogenous organ-specific multipotent SCs, or alternatively they can be adult differentiated somatic cells, reprogrammed to IPS cells. SCs are isolated, expanded and differentiated to the cell type of interest in an appropriate culture medium, seeded in a scaffold, and cultured in vitro. At this point, the new tissue is implanted in the patient.
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Related In: Results  -  Collection


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fig1: Basic scheme of tissue engineering. A biopsy is carried out to extract cells from the patient. These can be endogenous organ-specific multipotent SCs, or alternatively they can be adult differentiated somatic cells, reprogrammed to IPS cells. SCs are isolated, expanded and differentiated to the cell type of interest in an appropriate culture medium, seeded in a scaffold, and cultured in vitro. At this point, the new tissue is implanted in the patient.

Mentions: The availability of sources of pluripotent SC has increased immensely the potential of cell therapy in medicine and opens up new perspectives in the treatment of diseases [47, 48]. IPS cells can proliferate and be induced to differentiate to a particular cell type, and the selected cells can be seeded in a specific mould or scaffold and cultured in vitro. Scaffolds (natural or synthetic) may be composed of polymers, metals, ceramics, or composites [49, 50]. Bioreactors are used to grow the cells on the scaffolds until the tissue or the organ is fully developed [41]. The cells can be expanded in culture and then reimplanted in the patient [51, 52] (Figure 1). The cells can come from the same individual (autologous) or the same species but from a different individual (allogeneic) or even can originate from different species (heterologous).


Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering.

Ibarretxe G, Alvarez A, CaƱavate ML, Hilario E, Aurrekoetxea M, Unda F - Stem Cells Int (2012)

Basic scheme of tissue engineering. A biopsy is carried out to extract cells from the patient. These can be endogenous organ-specific multipotent SCs, or alternatively they can be adult differentiated somatic cells, reprogrammed to IPS cells. SCs are isolated, expanded and differentiated to the cell type of interest in an appropriate culture medium, seeded in a scaffold, and cultured in vitro. At this point, the new tissue is implanted in the patient.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368489&req=5

fig1: Basic scheme of tissue engineering. A biopsy is carried out to extract cells from the patient. These can be endogenous organ-specific multipotent SCs, or alternatively they can be adult differentiated somatic cells, reprogrammed to IPS cells. SCs are isolated, expanded and differentiated to the cell type of interest in an appropriate culture medium, seeded in a scaffold, and cultured in vitro. At this point, the new tissue is implanted in the patient.
Mentions: The availability of sources of pluripotent SC has increased immensely the potential of cell therapy in medicine and opens up new perspectives in the treatment of diseases [47, 48]. IPS cells can proliferate and be induced to differentiate to a particular cell type, and the selected cells can be seeded in a specific mould or scaffold and cultured in vitro. Scaffolds (natural or synthetic) may be composed of polymers, metals, ceramics, or composites [49, 50]. Bioreactors are used to grow the cells on the scaffolds until the tissue or the organ is fully developed [41]. The cells can be expanded in culture and then reimplanted in the patient [51, 52] (Figure 1). The cells can come from the same individual (autologous) or the same species but from a different individual (allogeneic) or even can originate from different species (heterologous).

Bottom Line: The potential of IPS cell technology is tremendous, but it will be essential to improve the methodologies for IPS cell generation and to precisely evaluate each clone and subclone of IPS cells for their safety and efficacy.Additionally, the current state of knowledge on IPS cells advises that research on their regenerative properties is carried out in appropriate tissue and organ systems that permit a safe assessment of the long-term behavior of these reprogrammed cells.In the present paper, we discuss the mechanisms of cell reprogramming, current technical limitations of IPS cells for their use in human tissue engineering, and possibilities to overcome them in the particular case of dental regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and Histology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain.

ABSTRACT
The procurement of induced pluripotent stem cells, or IPS cells, from adult differentiated animal cells has the potential to revolutionize future medicine, where reprogrammed IPS cells may be used to repair disease-affected tissues on demand. The potential of IPS cell technology is tremendous, but it will be essential to improve the methodologies for IPS cell generation and to precisely evaluate each clone and subclone of IPS cells for their safety and efficacy. Additionally, the current state of knowledge on IPS cells advises that research on their regenerative properties is carried out in appropriate tissue and organ systems that permit a safe assessment of the long-term behavior of these reprogrammed cells. In the present paper, we discuss the mechanisms of cell reprogramming, current technical limitations of IPS cells for their use in human tissue engineering, and possibilities to overcome them in the particular case of dental regeneration.

No MeSH data available.